Ciona intestinalis as a Marine Model System to Study Some Key Developmental Genes Targeted by the Diatom-Derived Aldehyde Decadienal
The anti-proliferative effects of diatoms, described for the first time in copepods, have also been demonstrated in benthic invertebrates such as polychaetes, sea urchins and tunicates. In these organisms PUAs (polyunsaturated aldehydes) induce the disruption of gametogenesis, gamete functionality,...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-03-01
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| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1660-3397/13/3/1451 |
| _version_ | 1798005421786529792 |
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| author | Anna Lettieri Rosaria Esposito Adrianna Ianora Antonietta Spagnuolo |
| author_facet | Anna Lettieri Rosaria Esposito Adrianna Ianora Antonietta Spagnuolo |
| author_sort | Anna Lettieri |
| collection | DOAJ |
| description | The anti-proliferative effects of diatoms, described for the first time in copepods, have also been demonstrated in benthic invertebrates such as polychaetes, sea urchins and tunicates. In these organisms PUAs (polyunsaturated aldehydes) induce the disruption of gametogenesis, gamete functionality, fertilization, embryonic mitosis, and larval fitness and competence. These inhibitory effects are due to the PUAs, produced by diatoms in response to physical damage as occurs during copepod grazing. The cell targets of these compounds remain largely unknown. Here we identify some of the genes targeted by the diatom PUA 2-trans-4-trans-decadienal (DD) using the tunicate Ciona intestinalis. The tools, techniques and genomic resources available for Ciona, as well as the suitability of Ciona embryos for medium-to high-throughput strategies, are key to their employment as model organisms in different fields, including the investigation of toxic agents that could interfere with developmental processes. We demonstrate that DD can induce developmental aberrations in Ciona larvae in a dose-dependent manner. Moreover, through a preliminary analysis, DD is shown to affect the expression level of genes involved in stress response and developmental processes. |
| first_indexed | 2024-04-11T12:39:58Z |
| format | Article |
| id | doaj.art-9e487c7b3ddb4202a1e092bb0d576358 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T12:39:58Z |
| publishDate | 2015-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-9e487c7b3ddb4202a1e092bb0d5763582022-12-22T04:23:32ZengMDPI AGMarine Drugs1660-33972015-03-011331451146510.3390/md13031451md13031451Ciona intestinalis as a Marine Model System to Study Some Key Developmental Genes Targeted by the Diatom-Derived Aldehyde DecadienalAnna Lettieri0Rosaria Esposito1Adrianna Ianora2Antonietta Spagnuolo3Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 NAPOLI, ItalyStazione Zoologica Anton Dohrn, Villa Comunale, 80121 NAPOLI, ItalyStazione Zoologica Anton Dohrn, Villa Comunale, 80121 NAPOLI, ItalyStazione Zoologica Anton Dohrn, Villa Comunale, 80121 NAPOLI, ItalyThe anti-proliferative effects of diatoms, described for the first time in copepods, have also been demonstrated in benthic invertebrates such as polychaetes, sea urchins and tunicates. In these organisms PUAs (polyunsaturated aldehydes) induce the disruption of gametogenesis, gamete functionality, fertilization, embryonic mitosis, and larval fitness and competence. These inhibitory effects are due to the PUAs, produced by diatoms in response to physical damage as occurs during copepod grazing. The cell targets of these compounds remain largely unknown. Here we identify some of the genes targeted by the diatom PUA 2-trans-4-trans-decadienal (DD) using the tunicate Ciona intestinalis. The tools, techniques and genomic resources available for Ciona, as well as the suitability of Ciona embryos for medium-to high-throughput strategies, are key to their employment as model organisms in different fields, including the investigation of toxic agents that could interfere with developmental processes. We demonstrate that DD can induce developmental aberrations in Ciona larvae in a dose-dependent manner. Moreover, through a preliminary analysis, DD is shown to affect the expression level of genes involved in stress response and developmental processes.http://www.mdpi.com/1660-3397/13/3/1451ascidianHoxParaHoxstressglutathione (GSH)development |
| spellingShingle | Anna Lettieri Rosaria Esposito Adrianna Ianora Antonietta Spagnuolo Ciona intestinalis as a Marine Model System to Study Some Key Developmental Genes Targeted by the Diatom-Derived Aldehyde Decadienal Marine Drugs ascidian Hox ParaHox stress glutathione (GSH) development |
| title | Ciona intestinalis as a Marine Model System to Study Some Key Developmental Genes Targeted by the Diatom-Derived Aldehyde Decadienal |
| title_full | Ciona intestinalis as a Marine Model System to Study Some Key Developmental Genes Targeted by the Diatom-Derived Aldehyde Decadienal |
| title_fullStr | Ciona intestinalis as a Marine Model System to Study Some Key Developmental Genes Targeted by the Diatom-Derived Aldehyde Decadienal |
| title_full_unstemmed | Ciona intestinalis as a Marine Model System to Study Some Key Developmental Genes Targeted by the Diatom-Derived Aldehyde Decadienal |
| title_short | Ciona intestinalis as a Marine Model System to Study Some Key Developmental Genes Targeted by the Diatom-Derived Aldehyde Decadienal |
| title_sort | ciona intestinalis as a marine model system to study some key developmental genes targeted by the diatom derived aldehyde decadienal |
| topic | ascidian Hox ParaHox stress glutathione (GSH) development |
| url | http://www.mdpi.com/1660-3397/13/3/1451 |
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