Scanning Probe Microscopy Techniques for Studying the Cell Glycocalyx
The glycocalyx is a brush-like layer that covers the surfaces of the membranes of most cell types. It consists of a mixture of carbohydrates, mainly glycoproteins and proteoglycans. Due to its structure and sensitivity to environmental conditions, it represents a complicated object to investigate. H...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/12/24/2778 |
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| author | Dmitry Kolesov Anna Astakhova Maria Galdobina Alexey Moskovtsev Aslan Kubatiev Alisa Sokolovskaya Leonid Ukrainskiy Sergey Morozov |
| author_facet | Dmitry Kolesov Anna Astakhova Maria Galdobina Alexey Moskovtsev Aslan Kubatiev Alisa Sokolovskaya Leonid Ukrainskiy Sergey Morozov |
| author_sort | Dmitry Kolesov |
| collection | DOAJ |
| description | The glycocalyx is a brush-like layer that covers the surfaces of the membranes of most cell types. It consists of a mixture of carbohydrates, mainly glycoproteins and proteoglycans. Due to its structure and sensitivity to environmental conditions, it represents a complicated object to investigate. Here, we review studies of the glycocalyx conducted using scanning probe microscopy approaches. This includes imaging techniques as well as the measurement of nanomechanical properties. The nanomechanics of the glycocalyx is particularly important since it is widely present on the surfaces of mechanosensitive cells such as endothelial cells. An overview of problems with the interpretation of indirect data via the use of analytical models is presented. Special insight is given into changes in glycocalyx properties during pathological processes. The biological background and alternative research methods are briefly covered. |
| first_indexed | 2024-03-08T20:54:56Z |
| format | Article |
| id | doaj.art-9e492cc770e04c9795286f3c180e9847 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-08T20:54:56Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-9e492cc770e04c9795286f3c180e98472023-12-22T13:59:33ZengMDPI AGCells2073-44092023-12-011224277810.3390/cells12242778Scanning Probe Microscopy Techniques for Studying the Cell GlycocalyxDmitry Kolesov0Anna Astakhova1Maria Galdobina2Alexey Moskovtsev3Aslan Kubatiev4Alisa Sokolovskaya5Leonid Ukrainskiy6Sergey Morozov7Moscow Polytechnic University, 107023 Moscow, RussiaInstitute of General Pathology and Pathophysiology, 125315 Moscow, RussiaInstitute of General Pathology and Pathophysiology, 125315 Moscow, RussiaInstitute of General Pathology and Pathophysiology, 125315 Moscow, RussiaInstitute of General Pathology and Pathophysiology, 125315 Moscow, RussiaInstitute of General Pathology and Pathophysiology, 125315 Moscow, RussiaMechanical Engineering Research Institute of the Russian Academy of Sciences, 119334 Moscow, RussiaInstitute of General Pathology and Pathophysiology, 125315 Moscow, RussiaThe glycocalyx is a brush-like layer that covers the surfaces of the membranes of most cell types. It consists of a mixture of carbohydrates, mainly glycoproteins and proteoglycans. Due to its structure and sensitivity to environmental conditions, it represents a complicated object to investigate. Here, we review studies of the glycocalyx conducted using scanning probe microscopy approaches. This includes imaging techniques as well as the measurement of nanomechanical properties. The nanomechanics of the glycocalyx is particularly important since it is widely present on the surfaces of mechanosensitive cells such as endothelial cells. An overview of problems with the interpretation of indirect data via the use of analytical models is presented. Special insight is given into changes in glycocalyx properties during pathological processes. The biological background and alternative research methods are briefly covered.https://www.mdpi.com/2073-4409/12/24/2778glycocalyxscanning probe microscopyatomic force nanoindentationcell nanomechanics |
| spellingShingle | Dmitry Kolesov Anna Astakhova Maria Galdobina Alexey Moskovtsev Aslan Kubatiev Alisa Sokolovskaya Leonid Ukrainskiy Sergey Morozov Scanning Probe Microscopy Techniques for Studying the Cell Glycocalyx Cells glycocalyx scanning probe microscopy atomic force nanoindentation cell nanomechanics |
| title | Scanning Probe Microscopy Techniques for Studying the Cell Glycocalyx |
| title_full | Scanning Probe Microscopy Techniques for Studying the Cell Glycocalyx |
| title_fullStr | Scanning Probe Microscopy Techniques for Studying the Cell Glycocalyx |
| title_full_unstemmed | Scanning Probe Microscopy Techniques for Studying the Cell Glycocalyx |
| title_short | Scanning Probe Microscopy Techniques for Studying the Cell Glycocalyx |
| title_sort | scanning probe microscopy techniques for studying the cell glycocalyx |
| topic | glycocalyx scanning probe microscopy atomic force nanoindentation cell nanomechanics |
| url | https://www.mdpi.com/2073-4409/12/24/2778 |
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