Circular RNA Circ_0067934 Attenuates Ferroptosis of Thyroid Cancer Cells by miR-545-3p/SLC7A11 Signaling
Ferroptosis is an emerging programmed cell death distinguished from apoptosis and autophagy and plays essential roles in tumorigenesis. Thyroid cancer is a prevalent endocrine tumor, but the molecular mechanism of ferroptosis during thyroid cancer development remains unclear. Here, we identified the...
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2021.670031/full |
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| author | Hui-Hui Wang Hui-Hui Wang Hui-Hui Wang Jia-Ni Ma Xiao-Rong Zhan Xiao-Rong Zhan |
| author_facet | Hui-Hui Wang Hui-Hui Wang Hui-Hui Wang Jia-Ni Ma Xiao-Rong Zhan Xiao-Rong Zhan |
| author_sort | Hui-Hui Wang |
| collection | DOAJ |
| description | Ferroptosis is an emerging programmed cell death distinguished from apoptosis and autophagy and plays essential roles in tumorigenesis. Thyroid cancer is a prevalent endocrine tumor, but the molecular mechanism of ferroptosis during thyroid cancer development remains unclear. Here, we identified the critical function of circular RNA circ_0067934 in repressing ferroptosis of thyroid cancer cells. Our data showed that the ferroptosis activator erastin decreased thyroid cancer cell viabilities, while the circ_0067934 shRNA further attenuated erastin-inhibited cell viabilities. The silencing of circ_0067934 enhanced the levels of ferroptosis-related markers, including Fe2+, iron, and ROS in the cells. The knockdown of circ_0067934 induced thyroid cancer cell apoptosis and repressed thyroid cancer cell proliferation in vitro and in vivo. Circ_0067934 upregulated the expression of the ferroptosis-negative regulator SLC7A11 by sponging and inhibiting miR-545-3p in thyroid cancer cells. The overexpression of SLC7A11 or the inhibitor of miR-545-3p reversed circ_0067934 silencing-regulated thyroid cancer cell proliferation. Therefore, we concluded that Circ_0067934 attenuated ferroptosis of thyroid cancer cells by miR-545-3p/SLC7A11 signaling. Circ_0067934 may serve as a potential therapeutic target by regulating ferroptosis for the treatment of thyroid cancer. |
| first_indexed | 2024-12-17T03:06:40Z |
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| id | doaj.art-9e4b4d7e75354ea4a7049181f0e92816 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-12-17T03:06:40Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-9e4b4d7e75354ea4a7049181f0e928162022-12-21T22:05:55ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922021-07-011210.3389/fendo.2021.670031670031Circular RNA Circ_0067934 Attenuates Ferroptosis of Thyroid Cancer Cells by miR-545-3p/SLC7A11 SignalingHui-Hui Wang0Hui-Hui Wang1Hui-Hui Wang2Jia-Ni Ma3Xiao-Rong Zhan4Xiao-Rong Zhan5Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Endocrinology, Qiqihar First Hospital, Qiqihar, ChinaAffiliated Qiqihar Hospital, Southern Medical University, Qiqihar, ChinaDepartment of Endocrinology, Qiqihar First Hospital, Qiqihar, ChinaDepartment of Endocrinology, The First Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Endocrinology, Qiqihar First Hospital, Qiqihar, ChinaFerroptosis is an emerging programmed cell death distinguished from apoptosis and autophagy and plays essential roles in tumorigenesis. Thyroid cancer is a prevalent endocrine tumor, but the molecular mechanism of ferroptosis during thyroid cancer development remains unclear. Here, we identified the critical function of circular RNA circ_0067934 in repressing ferroptosis of thyroid cancer cells. Our data showed that the ferroptosis activator erastin decreased thyroid cancer cell viabilities, while the circ_0067934 shRNA further attenuated erastin-inhibited cell viabilities. The silencing of circ_0067934 enhanced the levels of ferroptosis-related markers, including Fe2+, iron, and ROS in the cells. The knockdown of circ_0067934 induced thyroid cancer cell apoptosis and repressed thyroid cancer cell proliferation in vitro and in vivo. Circ_0067934 upregulated the expression of the ferroptosis-negative regulator SLC7A11 by sponging and inhibiting miR-545-3p in thyroid cancer cells. The overexpression of SLC7A11 or the inhibitor of miR-545-3p reversed circ_0067934 silencing-regulated thyroid cancer cell proliferation. Therefore, we concluded that Circ_0067934 attenuated ferroptosis of thyroid cancer cells by miR-545-3p/SLC7A11 signaling. Circ_0067934 may serve as a potential therapeutic target by regulating ferroptosis for the treatment of thyroid cancer.https://www.frontiersin.org/articles/10.3389/fendo.2021.670031/fullthyroid cancerferroptosiscirc_0067934miR-545-3pSLC7A11 |
| spellingShingle | Hui-Hui Wang Hui-Hui Wang Hui-Hui Wang Jia-Ni Ma Xiao-Rong Zhan Xiao-Rong Zhan Circular RNA Circ_0067934 Attenuates Ferroptosis of Thyroid Cancer Cells by miR-545-3p/SLC7A11 Signaling Frontiers in Endocrinology thyroid cancer ferroptosis circ_0067934 miR-545-3p SLC7A11 |
| title | Circular RNA Circ_0067934 Attenuates Ferroptosis of Thyroid Cancer Cells by miR-545-3p/SLC7A11 Signaling |
| title_full | Circular RNA Circ_0067934 Attenuates Ferroptosis of Thyroid Cancer Cells by miR-545-3p/SLC7A11 Signaling |
| title_fullStr | Circular RNA Circ_0067934 Attenuates Ferroptosis of Thyroid Cancer Cells by miR-545-3p/SLC7A11 Signaling |
| title_full_unstemmed | Circular RNA Circ_0067934 Attenuates Ferroptosis of Thyroid Cancer Cells by miR-545-3p/SLC7A11 Signaling |
| title_short | Circular RNA Circ_0067934 Attenuates Ferroptosis of Thyroid Cancer Cells by miR-545-3p/SLC7A11 Signaling |
| title_sort | circular rna circ 0067934 attenuates ferroptosis of thyroid cancer cells by mir 545 3p slc7a11 signaling |
| topic | thyroid cancer ferroptosis circ_0067934 miR-545-3p SLC7A11 |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2021.670031/full |
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