Increased CXCL10 Expression in Nasal Fibroblasts from Patients with Refractory Chronic Rhinosinusitis and Asthma
Background: Chronic rhinosinusitis (CRS) is characterized by local inflammation of the sinonasal tissues. CRS patients with nasal polyps and asthma often develop acute exacerbation of sinonasal symptoms after upper respiratory tract infections. However, the influence of concomitant asthma on the nas...
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| Format: | Article |
| Language: | English |
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Elsevier
2013-01-01
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| Series: | Allergology International |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1323893015301684 |
| _version_ | 1828527504822894592 |
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| author | Mamoru Yoshikawa Kota Wada Tsuyoshi Yoshimura Daiya Asaka Naoko Okada Kenji Matsumoto Hiroshi Moriyama |
| author_facet | Mamoru Yoshikawa Kota Wada Tsuyoshi Yoshimura Daiya Asaka Naoko Okada Kenji Matsumoto Hiroshi Moriyama |
| author_sort | Mamoru Yoshikawa |
| collection | DOAJ |
| description | Background: Chronic rhinosinusitis (CRS) is characterized by local inflammation of the sinonasal tissues. CRS patients with nasal polyps and asthma often develop acute exacerbation of sinonasal symptoms after upper respiratory tract infections. However, the influence of concomitant asthma on the nasal immune response to viral infection remains unclear.
Methods: Specimens of nasal polyp and mucosal tissues were obtained from 3 groups of CRS patients (n = 14 per group): 1) patients without asthma (CRS group), 2) patients with aspirin-tolerant asthma (ATA group), and 3) patients with aspirin-intolerant asthma (AIA group). Nasal fibroblasts isolated from the specimens were stimulated with poly I:C. CXCL10 expression was analyzed by the quantitative real-time polymerase chain reaction and enzyme-linked immunoadsorbent assay. Biopsy specimens from CRS patients without asthma were subjected to immunohistochemistry for detection of T-bet and GATA-3 expression in CD3+ T cells by double labeling.
Results: Nasal fibroblasts from the ATA and AIA groups showed significantly enhanced expression of CXCL 10 mRNA and protein after poly I:C stimulation compared with cells from the CRS group and the control group (normal nasal mucosa). In addition to T helper (Th)2 cells, there was more abundant infiltration of Th1 cells into tissues from the AIA and ATA groups.
Conclusions: Our findings suggest that CRS associated with asthma may become intractable through the over-production of CXCL10 in response to viral infection. |
| first_indexed | 2024-12-11T21:39:27Z |
| format | Article |
| id | doaj.art-9e4c8359ce624b58883829ea54c5ea8b |
| institution | Directory Open Access Journal |
| issn | 1323-8930 |
| language | English |
| last_indexed | 2024-12-11T21:39:27Z |
| publishDate | 2013-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Allergology International |
| spelling | doaj.art-9e4c8359ce624b58883829ea54c5ea8b2022-12-22T00:49:53ZengElsevierAllergology International1323-89302013-01-0162449550210.2332/allergolint.13-OA-0572Increased CXCL10 Expression in Nasal Fibroblasts from Patients with Refractory Chronic Rhinosinusitis and AsthmaMamoru Yoshikawa0Kota Wada1Tsuyoshi Yoshimura2Daiya Asaka3Naoko Okada4Kenji Matsumoto5Hiroshi Moriyama6Department of Otorhinolaryngology, Toho University School of Medicine, Tokyo, Japan.Department of Otorhinolaryngology, Jikei University School of Medicine , Tokyo, Japan.Department of Otorhinolaryngology, Jikei University School of Medicine , Tokyo, Japan.Department of Otorhinolaryngology, Jikei University School of Medicine , Tokyo, Japan.Department of Allergy and Immunology, National Research Institute for Child Health and Development, Tokyo, Japan.Department of Allergy and Immunology, National Research Institute for Child Health and Development, Tokyo, Japan.Department of Otorhinolaryngology, Jikei University School of Medicine , Tokyo, Japan.Background: Chronic rhinosinusitis (CRS) is characterized by local inflammation of the sinonasal tissues. CRS patients with nasal polyps and asthma often develop acute exacerbation of sinonasal symptoms after upper respiratory tract infections. However, the influence of concomitant asthma on the nasal immune response to viral infection remains unclear. Methods: Specimens of nasal polyp and mucosal tissues were obtained from 3 groups of CRS patients (n = 14 per group): 1) patients without asthma (CRS group), 2) patients with aspirin-tolerant asthma (ATA group), and 3) patients with aspirin-intolerant asthma (AIA group). Nasal fibroblasts isolated from the specimens were stimulated with poly I:C. CXCL10 expression was analyzed by the quantitative real-time polymerase chain reaction and enzyme-linked immunoadsorbent assay. Biopsy specimens from CRS patients without asthma were subjected to immunohistochemistry for detection of T-bet and GATA-3 expression in CD3+ T cells by double labeling. Results: Nasal fibroblasts from the ATA and AIA groups showed significantly enhanced expression of CXCL 10 mRNA and protein after poly I:C stimulation compared with cells from the CRS group and the control group (normal nasal mucosa). In addition to T helper (Th)2 cells, there was more abundant infiltration of Th1 cells into tissues from the AIA and ATA groups. Conclusions: Our findings suggest that CRS associated with asthma may become intractable through the over-production of CXCL10 in response to viral infection.http://www.sciencedirect.com/science/article/pii/S1323893015301684asthmaCXCL10 chemokinefibroblastnasal polypsrhinosinusitis |
| spellingShingle | Mamoru Yoshikawa Kota Wada Tsuyoshi Yoshimura Daiya Asaka Naoko Okada Kenji Matsumoto Hiroshi Moriyama Increased CXCL10 Expression in Nasal Fibroblasts from Patients with Refractory Chronic Rhinosinusitis and Asthma Allergology International asthma CXCL10 chemokine fibroblast nasal polyps rhinosinusitis |
| title | Increased CXCL10 Expression in Nasal Fibroblasts from Patients with Refractory Chronic Rhinosinusitis and Asthma |
| title_full | Increased CXCL10 Expression in Nasal Fibroblasts from Patients with Refractory Chronic Rhinosinusitis and Asthma |
| title_fullStr | Increased CXCL10 Expression in Nasal Fibroblasts from Patients with Refractory Chronic Rhinosinusitis and Asthma |
| title_full_unstemmed | Increased CXCL10 Expression in Nasal Fibroblasts from Patients with Refractory Chronic Rhinosinusitis and Asthma |
| title_short | Increased CXCL10 Expression in Nasal Fibroblasts from Patients with Refractory Chronic Rhinosinusitis and Asthma |
| title_sort | increased cxcl10 expression in nasal fibroblasts from patients with refractory chronic rhinosinusitis and asthma |
| topic | asthma CXCL10 chemokine fibroblast nasal polyps rhinosinusitis |
| url | http://www.sciencedirect.com/science/article/pii/S1323893015301684 |
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