Carbohydrate co-ingestion with protein does not further augment post-prandial muscle protein accretion in older men
<p>Abstract</p> <p>Background</p> <p>A blunted muscle protein synthetic response to protein ingestion may contribute to the age related loss of muscle tissue. We hypothesized that the greater endogenous insulin release following co-ingestion of carbohydrate facilitates...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2013-01-01
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| Series: | Nutrition & Metabolism |
| Subjects: | |
| Online Access: | http://www.nutritionandmetabolism.com/content/10/1/15 |
| _version_ | 1818664294863077376 |
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| author | Hamer Henrike M Wall Benjamin T Kiskini Alexandra de Lange Anneke Groen Bart BL Bakker Jaap A Gijsen Annemie P Verdijk Lex B van Loon Luc JC |
| author_facet | Hamer Henrike M Wall Benjamin T Kiskini Alexandra de Lange Anneke Groen Bart BL Bakker Jaap A Gijsen Annemie P Verdijk Lex B van Loon Luc JC |
| author_sort | Hamer Henrike M |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>A blunted muscle protein synthetic response to protein ingestion may contribute to the age related loss of muscle tissue. We hypothesized that the greater endogenous insulin release following co-ingestion of carbohydrate facilitates post-prandial muscle protein accretion after ingesting a meal-like bolus of protein in older males.</p> <p>Methods</p> <p>Twenty-four healthy older men (75±1 y) were randomly assigned to ingest 20 g intrinsically L-[1-<sup>13</sup>C] phenylalanine-labeled casein protein with (PRO-CHO) or without (PRO) 40 g carbohydrate. Ingestion of specifically produced intrinsically L-[1-<sup>13</sup>C] phenylalanine labeled protein allowed us to assess post-prandial incorporation of dietary protein derived amino acids into muscle protein. Blood samples were collected at regular intervals, with muscle biopsies being obtained prior to and 2 and 6 h after protein ingestion.</p> <p>Results</p> <p>Plasma glucose and insulin concentrations showed a greater increase in PRO-CHO compared with PRO (<it>P</it><0.001). Muscle protein-bound L-[1-<sup>13</sup>C] phenylalanine enrichments tended to increase to a greater extent in PRO-CHO compared with PRO during the first 2 h after protein ingestion (0.0072±0.0013 vs 0.0046±0.010 MPE, respectively; <it>P</it>=0.13). However, 6 h after protein ingestion, differences in muscle protein-bound L-[1-<sup>13</sup>C] phenylalanine enrichments were no longer observed between experiments (0.0213±0.0024 vs 0.0185±0.0010 MPE, respectively; <it>P</it>=0.30).</p> <p>Conclusions</p> <p>This study shows that carbohydrate ingestion may accelerate, but does not further augment post-prandial incorporation of dietary protein derived amino acids into muscle protein in healthy elderly men.</p> |
| first_indexed | 2024-12-17T05:30:28Z |
| format | Article |
| id | doaj.art-9e4d3c70e4874661b114a2e0dda9ca43 |
| institution | Directory Open Access Journal |
| issn | 1743-7075 |
| language | English |
| last_indexed | 2024-12-17T05:30:28Z |
| publishDate | 2013-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Nutrition & Metabolism |
| spelling | doaj.art-9e4d3c70e4874661b114a2e0dda9ca432022-12-21T22:01:45ZengBMCNutrition & Metabolism1743-70752013-01-011011510.1186/1743-7075-10-15Carbohydrate co-ingestion with protein does not further augment post-prandial muscle protein accretion in older menHamer Henrike MWall Benjamin TKiskini Alexandrade Lange AnnekeGroen Bart BLBakker Jaap AGijsen Annemie PVerdijk Lex Bvan Loon Luc JC<p>Abstract</p> <p>Background</p> <p>A blunted muscle protein synthetic response to protein ingestion may contribute to the age related loss of muscle tissue. We hypothesized that the greater endogenous insulin release following co-ingestion of carbohydrate facilitates post-prandial muscle protein accretion after ingesting a meal-like bolus of protein in older males.</p> <p>Methods</p> <p>Twenty-four healthy older men (75±1 y) were randomly assigned to ingest 20 g intrinsically L-[1-<sup>13</sup>C] phenylalanine-labeled casein protein with (PRO-CHO) or without (PRO) 40 g carbohydrate. Ingestion of specifically produced intrinsically L-[1-<sup>13</sup>C] phenylalanine labeled protein allowed us to assess post-prandial incorporation of dietary protein derived amino acids into muscle protein. Blood samples were collected at regular intervals, with muscle biopsies being obtained prior to and 2 and 6 h after protein ingestion.</p> <p>Results</p> <p>Plasma glucose and insulin concentrations showed a greater increase in PRO-CHO compared with PRO (<it>P</it><0.001). Muscle protein-bound L-[1-<sup>13</sup>C] phenylalanine enrichments tended to increase to a greater extent in PRO-CHO compared with PRO during the first 2 h after protein ingestion (0.0072±0.0013 vs 0.0046±0.010 MPE, respectively; <it>P</it>=0.13). However, 6 h after protein ingestion, differences in muscle protein-bound L-[1-<sup>13</sup>C] phenylalanine enrichments were no longer observed between experiments (0.0213±0.0024 vs 0.0185±0.0010 MPE, respectively; <it>P</it>=0.30).</p> <p>Conclusions</p> <p>This study shows that carbohydrate ingestion may accelerate, but does not further augment post-prandial incorporation of dietary protein derived amino acids into muscle protein in healthy elderly men.</p>http://www.nutritionandmetabolism.com/content/10/1/15Skeletal muscleAgeingSarcopeniaAmino acidsAnabolic resistance |
| spellingShingle | Hamer Henrike M Wall Benjamin T Kiskini Alexandra de Lange Anneke Groen Bart BL Bakker Jaap A Gijsen Annemie P Verdijk Lex B van Loon Luc JC Carbohydrate co-ingestion with protein does not further augment post-prandial muscle protein accretion in older men Nutrition & Metabolism Skeletal muscle Ageing Sarcopenia Amino acids Anabolic resistance |
| title | Carbohydrate co-ingestion with protein does not further augment post-prandial muscle protein accretion in older men |
| title_full | Carbohydrate co-ingestion with protein does not further augment post-prandial muscle protein accretion in older men |
| title_fullStr | Carbohydrate co-ingestion with protein does not further augment post-prandial muscle protein accretion in older men |
| title_full_unstemmed | Carbohydrate co-ingestion with protein does not further augment post-prandial muscle protein accretion in older men |
| title_short | Carbohydrate co-ingestion with protein does not further augment post-prandial muscle protein accretion in older men |
| title_sort | carbohydrate co ingestion with protein does not further augment post prandial muscle protein accretion in older men |
| topic | Skeletal muscle Ageing Sarcopenia Amino acids Anabolic resistance |
| url | http://www.nutritionandmetabolism.com/content/10/1/15 |
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