Antioxidant, Cytotoxicity and Cytoprotective Potential of Extracts of Grewia Flava and Grewia Bicolor Berries
Objectives: Accumulation of cellular reactive oxygen species (ROS) leads to oxidative stress. Increased production of ROS, such as superoxide anion, or a deficiency in their clearance by antioxidant defences, mediates cellular pathology. Grewia Spp fruits are a source of bioactive compounds and have...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Korean Pharmacopuncture Institute
2021-03-01
|
| Series: | Journal of Pharmacopuncture |
| Subjects: | |
| Online Access: | http://www.journal-jop.org/journal/view.html?doi=10.3831/KPI.2021.24.1.24 |
| _version_ | 1818726194018779136 |
|---|---|
| author | Kabo Masisi Riach Masamba Keletso Lashani Chunyang Li Tebogo E. Kwape Goabaone Gaobotse |
| author_facet | Kabo Masisi Riach Masamba Keletso Lashani Chunyang Li Tebogo E. Kwape Goabaone Gaobotse |
| author_sort | Kabo Masisi |
| collection | DOAJ |
| description | Objectives: Accumulation of cellular reactive oxygen species (ROS) leads to oxidative stress. Increased production of ROS, such as superoxide anion, or a deficiency in their clearance by antioxidant defences, mediates cellular pathology. Grewia Spp fruits are a source of bioactive compounds and have notable antioxidant activity. Although the antioxidant capacity of Grewia Spp has been studied, there is very limited evidence that links the antioxidant activities of Grewia bicolor and Grewia flava to the inhibition of free radical formation associated with damage in biological systems.Methods : : This study evaluated the protective effects of Grewia bicolor and Grewia flava extracts against free radical-induced oxidative stress and the resulting cytotoxicity effect using HeLa cells. Antioxidant properties determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and total phenolic content (TPC) assays showed significantly higher (p < 0.05) antioxidant activity in Grewia flava (ethanol extract) than Grewia flava (water extract) and Grewia bicolor (ethanol and water extracts).Results : : Using 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide or MTT assay, cytotoxicity results showed that extracts of Grewia bicolor and Grewia flava were less toxic to HeLa cells at tested concentrations compared to the untreated control. This confirmed the low toxicity of these edible fruits at the tested concentrations in HeLa cells. Furthermore, hydrogen peroxide (H2O2)-induced cell loss was effectively reduced by pre-incubating HeLa cells with Grewia bicolor and Grewia flava extracts, with Grewia flava (ethanol extract) revealing better protection.Conclusion : : The effect was speculated to be associated with the higher antioxidant activity of Grewia flava (ethanol extract). Additional studies will warrant confirmation of the mechanism of action of such effects. |
| first_indexed | 2024-12-17T21:54:19Z |
| format | Article |
| id | doaj.art-9e4f447cad534acc81dbcc30b4a407c9 |
| institution | Directory Open Access Journal |
| issn | 2093-6966 |
| language | English |
| last_indexed | 2024-12-17T21:54:19Z |
| publishDate | 2021-03-01 |
| publisher | Korean Pharmacopuncture Institute |
| record_format | Article |
| series | Journal of Pharmacopuncture |
| spelling | doaj.art-9e4f447cad534acc81dbcc30b4a407c92022-12-21T21:31:10ZengKorean Pharmacopuncture InstituteJournal of Pharmacopuncture2093-69662021-03-01241243110.3831/KPI.2021.24.1.24KPI.2021.24.1.24Antioxidant, Cytotoxicity and Cytoprotective Potential of Extracts of Grewia Flava and Grewia Bicolor BerriesKabo Masisi0Riach Masamba1Keletso Lashani2Chunyang Li3Tebogo E. Kwape4Goabaone Gaobotse5Department of Biological Sciences and Biotechnology, Faculty of Sciences, Botswana International University of Science and Technology, Palapye, BotswanaDepartment of Biological Sciences and Biotechnology, Faculty of Sciences, Botswana International University of Science and Technology, Palapye, BotswanaDepartment of Biological Sciences and Biotechnology, Faculty of Sciences, Botswana International University of Science and Technology, Palapye, BotswanaDepartment of Nutrition and Health, Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, ChinaDepartment of Biological Sciences and Biotechnology, Faculty of Sciences, Botswana International University of Science and Technology, Palapye, BotswanaDepartment of Biological Sciences and Biotechnology, Faculty of Sciences, Botswana International University of Science and Technology, Palapye, BotswanaObjectives: Accumulation of cellular reactive oxygen species (ROS) leads to oxidative stress. Increased production of ROS, such as superoxide anion, or a deficiency in their clearance by antioxidant defences, mediates cellular pathology. Grewia Spp fruits are a source of bioactive compounds and have notable antioxidant activity. Although the antioxidant capacity of Grewia Spp has been studied, there is very limited evidence that links the antioxidant activities of Grewia bicolor and Grewia flava to the inhibition of free radical formation associated with damage in biological systems.Methods : : This study evaluated the protective effects of Grewia bicolor and Grewia flava extracts against free radical-induced oxidative stress and the resulting cytotoxicity effect using HeLa cells. Antioxidant properties determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and total phenolic content (TPC) assays showed significantly higher (p < 0.05) antioxidant activity in Grewia flava (ethanol extract) than Grewia flava (water extract) and Grewia bicolor (ethanol and water extracts).Results : : Using 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide or MTT assay, cytotoxicity results showed that extracts of Grewia bicolor and Grewia flava were less toxic to HeLa cells at tested concentrations compared to the untreated control. This confirmed the low toxicity of these edible fruits at the tested concentrations in HeLa cells. Furthermore, hydrogen peroxide (H2O2)-induced cell loss was effectively reduced by pre-incubating HeLa cells with Grewia bicolor and Grewia flava extracts, with Grewia flava (ethanol extract) revealing better protection.Conclusion : : The effect was speculated to be associated with the higher antioxidant activity of Grewia flava (ethanol extract). Additional studies will warrant confirmation of the mechanism of action of such effects.http://www.journal-jop.org/journal/view.html?doi=10.3831/KPI.2021.24.1.24cytoprotectivereactive oxygen speciesantioxidantrewia bicolorcytotoxicitygrewia flava |
| spellingShingle | Kabo Masisi Riach Masamba Keletso Lashani Chunyang Li Tebogo E. Kwape Goabaone Gaobotse Antioxidant, Cytotoxicity and Cytoprotective Potential of Extracts of Grewia Flava and Grewia Bicolor Berries Journal of Pharmacopuncture cytoprotective reactive oxygen species antioxidant rewia bicolor cytotoxicity grewia flava |
| title | Antioxidant, Cytotoxicity and Cytoprotective Potential of Extracts of Grewia Flava and Grewia Bicolor Berries |
| title_full | Antioxidant, Cytotoxicity and Cytoprotective Potential of Extracts of Grewia Flava and Grewia Bicolor Berries |
| title_fullStr | Antioxidant, Cytotoxicity and Cytoprotective Potential of Extracts of Grewia Flava and Grewia Bicolor Berries |
| title_full_unstemmed | Antioxidant, Cytotoxicity and Cytoprotective Potential of Extracts of Grewia Flava and Grewia Bicolor Berries |
| title_short | Antioxidant, Cytotoxicity and Cytoprotective Potential of Extracts of Grewia Flava and Grewia Bicolor Berries |
| title_sort | antioxidant cytotoxicity and cytoprotective potential of extracts of grewia flava and grewia bicolor berries |
| topic | cytoprotective reactive oxygen species antioxidant rewia bicolor cytotoxicity grewia flava |
| url | http://www.journal-jop.org/journal/view.html?doi=10.3831/KPI.2021.24.1.24 |
| work_keys_str_mv | AT kabomasisi antioxidantcytotoxicityandcytoprotectivepotentialofextractsofgrewiaflavaandgrewiabicolorberries AT riachmasamba antioxidantcytotoxicityandcytoprotectivepotentialofextractsofgrewiaflavaandgrewiabicolorberries AT keletsolashani antioxidantcytotoxicityandcytoprotectivepotentialofextractsofgrewiaflavaandgrewiabicolorberries AT chunyangli antioxidantcytotoxicityandcytoprotectivepotentialofextractsofgrewiaflavaandgrewiabicolorberries AT tebogoekwape antioxidantcytotoxicityandcytoprotectivepotentialofextractsofgrewiaflavaandgrewiabicolorberries AT goabaonegaobotse antioxidantcytotoxicityandcytoprotectivepotentialofextractsofgrewiaflavaandgrewiabicolorberries |