SHOX2 DNA Methylation is a Biomarker for the diagnosis of lung cancer based on bronchial aspirates
<p>Abstract</p> <p>Background</p> <p>This study aimed to show that SHOX2 DNA methylation is a tumor marker in patients with suspected lung cancer by using bronchial fluid aspirated during bronchoscopy. Such a biomarker would be clinically valuable, especially when, foll...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-11-01
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| Series: | BMC Cancer |
| Online Access: | http://www.biomedcentral.com/1471-2407/10/600 |
| _version_ | 1818787137465614336 |
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| author | Liloglou Triantafillos Wille Ulrike Weickmann Sabine Tetzner Reimo Distler Jürgen Lewin Jörn Seemann Stefanie Flemming Nadja Seegebarth Anke Schlegel Thomas Kneip Christoph Dietrich Dimo Liebenberg Volker Schmidt Bernd Raji Olaide Walshaw Martin Fleischhacker Michael Witt Christian Field John K |
| author_facet | Liloglou Triantafillos Wille Ulrike Weickmann Sabine Tetzner Reimo Distler Jürgen Lewin Jörn Seemann Stefanie Flemming Nadja Seegebarth Anke Schlegel Thomas Kneip Christoph Dietrich Dimo Liebenberg Volker Schmidt Bernd Raji Olaide Walshaw Martin Fleischhacker Michael Witt Christian Field John K |
| author_sort | Liloglou Triantafillos |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>This study aimed to show that SHOX2 DNA methylation is a tumor marker in patients with suspected lung cancer by using bronchial fluid aspirated during bronchoscopy. Such a biomarker would be clinically valuable, especially when, following the first bronchoscopy, a final diagnosis cannot be established by histology or cytology. A test with a low false positive rate can reduce the need for further invasive and costly procedures and ensure early treatment.</p> <p>Methods</p> <p>Marker discovery was carried out by differential methylation hybridization (DMH) and real-time PCR. The real-time PCR based HeavyMethyl technology was used for quantitative analysis of DNA methylation of SHOX2 using bronchial aspirates from two clinical centres in a case-control study. Fresh-frozen and Saccomanno-fixed samples were used to show the tumor marker performance in different sample types of clinical relevance.</p> <p>Results</p> <p>Valid measurements were obtained from a total of 523 patient samples (242 controls, 281 cases). DNA methylation of SHOX2 allowed to distinguish between malignant and benign lung disease, i.e. abscesses, infections, obstructive lung diseases, sarcoidosis, scleroderma, stenoses, at high specificity (68% sensitivity [95% CI 62-73%], 95% specificity [95% CI 91-97%]).</p> <p>Conclusions</p> <p>Hypermethylation of SHOX2 in bronchial aspirates appears to be a clinically useful tumor marker for identifying subjects with lung carcinoma, especially if histological and cytological findings after bronchoscopy are ambiguous.</p> |
| first_indexed | 2024-12-18T14:02:59Z |
| format | Article |
| id | doaj.art-9e4f522dc19b4178aca16f83dfd49959 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-18T14:02:59Z |
| publishDate | 2010-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-9e4f522dc19b4178aca16f83dfd499592022-12-21T21:05:18ZengBMCBMC Cancer1471-24072010-11-0110160010.1186/1471-2407-10-600SHOX2 DNA Methylation is a Biomarker for the diagnosis of lung cancer based on bronchial aspiratesLiloglou TriantafillosWille UlrikeWeickmann SabineTetzner ReimoDistler JürgenLewin JörnSeemann StefanieFlemming NadjaSeegebarth AnkeSchlegel ThomasKneip ChristophDietrich DimoLiebenberg VolkerSchmidt BerndRaji OlaideWalshaw MartinFleischhacker MichaelWitt ChristianField John K<p>Abstract</p> <p>Background</p> <p>This study aimed to show that SHOX2 DNA methylation is a tumor marker in patients with suspected lung cancer by using bronchial fluid aspirated during bronchoscopy. Such a biomarker would be clinically valuable, especially when, following the first bronchoscopy, a final diagnosis cannot be established by histology or cytology. A test with a low false positive rate can reduce the need for further invasive and costly procedures and ensure early treatment.</p> <p>Methods</p> <p>Marker discovery was carried out by differential methylation hybridization (DMH) and real-time PCR. The real-time PCR based HeavyMethyl technology was used for quantitative analysis of DNA methylation of SHOX2 using bronchial aspirates from two clinical centres in a case-control study. Fresh-frozen and Saccomanno-fixed samples were used to show the tumor marker performance in different sample types of clinical relevance.</p> <p>Results</p> <p>Valid measurements were obtained from a total of 523 patient samples (242 controls, 281 cases). DNA methylation of SHOX2 allowed to distinguish between malignant and benign lung disease, i.e. abscesses, infections, obstructive lung diseases, sarcoidosis, scleroderma, stenoses, at high specificity (68% sensitivity [95% CI 62-73%], 95% specificity [95% CI 91-97%]).</p> <p>Conclusions</p> <p>Hypermethylation of SHOX2 in bronchial aspirates appears to be a clinically useful tumor marker for identifying subjects with lung carcinoma, especially if histological and cytological findings after bronchoscopy are ambiguous.</p>http://www.biomedcentral.com/1471-2407/10/600 |
| spellingShingle | Liloglou Triantafillos Wille Ulrike Weickmann Sabine Tetzner Reimo Distler Jürgen Lewin Jörn Seemann Stefanie Flemming Nadja Seegebarth Anke Schlegel Thomas Kneip Christoph Dietrich Dimo Liebenberg Volker Schmidt Bernd Raji Olaide Walshaw Martin Fleischhacker Michael Witt Christian Field John K SHOX2 DNA Methylation is a Biomarker for the diagnosis of lung cancer based on bronchial aspirates BMC Cancer |
| title | SHOX2 DNA Methylation is a Biomarker for the diagnosis of lung cancer based on bronchial aspirates |
| title_full | SHOX2 DNA Methylation is a Biomarker for the diagnosis of lung cancer based on bronchial aspirates |
| title_fullStr | SHOX2 DNA Methylation is a Biomarker for the diagnosis of lung cancer based on bronchial aspirates |
| title_full_unstemmed | SHOX2 DNA Methylation is a Biomarker for the diagnosis of lung cancer based on bronchial aspirates |
| title_short | SHOX2 DNA Methylation is a Biomarker for the diagnosis of lung cancer based on bronchial aspirates |
| title_sort | shox2 dna methylation is a biomarker for the diagnosis of lung cancer based on bronchial aspirates |
| url | http://www.biomedcentral.com/1471-2407/10/600 |
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