Elucidation of Bioactivity of Tricin Released by Thioacidolysis from Wheat Straw Lignin
Tricin is a complex compound with chemical bonds to phenylpropane units of lignin in gramineous plants, and it is predominantly bound to lignin by β-O-4 ether bonds. Thioacidolysis cleaves the alkyl aryl ether bonds, which releases tricin from the tricin-lignin complex and maintains the natural stru...
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| Format: | Article |
| Language: | English |
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North Carolina State University
2023-08-01
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| Series: | BioResources |
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| Online Access: | https://ojs.cnr.ncsu.edu/index.php/BRJ/article/view/22812 |
| _version_ | 1827821852980936704 |
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| author | Yunbo Zhao Junyi Zhou Hujun Niu Yimin Xie |
| author_facet | Yunbo Zhao Junyi Zhou Hujun Niu Yimin Xie |
| author_sort | Yunbo Zhao |
| collection | DOAJ |
| description | Tricin is a complex compound with chemical bonds to phenylpropane units of lignin in gramineous plants, and it is predominantly bound to lignin by β-O-4 ether bonds. Thioacidolysis cleaves the alkyl aryl ether bonds, which releases tricin from the tricin-lignin complex and maintains the natural structure of the tricin. In this study, milled wood lignin (MWL) was isolated from wheat straw by Bjӧrkman’s method, and the MWL was subjected to thioacidolysis to release tricin from the MWL. Medium-pressure preparative liquid chromatography was used for further purification. FT-IR and 1H-NMR analyses showed that the purified fraction was composed mainly of tricin-type flavonoids. The extracted tricin had strong scavenging capacity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals, with a half maximal inhibitory concentration (IC50) of 51.9 mg/L. Drug sensitivity paper testing showed that the extracted tricin inhibited the growth of Escherichia coli. The bacteriostatic circle diameters of tricin from wheat straw MWL and quercetin standard were 9.17 and 7.52 mm, respectively. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the tricin from wheat straw MWL had possible inhibitory effect on lung cancer A549 cells, with a maximum inhibitory performance of 44.5%. |
| first_indexed | 2024-03-12T01:49:30Z |
| format | Article |
| id | doaj.art-9e55b250aec645968f021b5330583b37 |
| institution | Directory Open Access Journal |
| issn | 1930-2126 |
| language | English |
| last_indexed | 2024-03-12T01:49:30Z |
| publishDate | 2023-08-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-9e55b250aec645968f021b5330583b372023-09-08T15:24:29ZengNorth Carolina State UniversityBioResources1930-21262023-08-0118472957308876Elucidation of Bioactivity of Tricin Released by Thioacidolysis from Wheat Straw LigninYunbo Zhao0Junyi Zhou1Hujun Niu2Yimin Xie3Research Institute of Pulp & Paper Engineering, Hubei University of Technology, 430068, Wuhan, ChinaResearch Institute of Pulp & Paper Engineering, Hubei University of Technology, 430068, Wuhan, ChinaResearch Institute of Pulp & Paper Engineering, Hubei University of Technology, 430068, Wuhan, ChinaResearch Institute of Pulp & Paper Engineering, Hubei University of Technology, 430068, Wuhan, China; Hubei Provincial Key Laboratory of Green Materials for Light Industry, Hubei University of Technology, 430068, Wuhan, ChinaTricin is a complex compound with chemical bonds to phenylpropane units of lignin in gramineous plants, and it is predominantly bound to lignin by β-O-4 ether bonds. Thioacidolysis cleaves the alkyl aryl ether bonds, which releases tricin from the tricin-lignin complex and maintains the natural structure of the tricin. In this study, milled wood lignin (MWL) was isolated from wheat straw by Bjӧrkman’s method, and the MWL was subjected to thioacidolysis to release tricin from the MWL. Medium-pressure preparative liquid chromatography was used for further purification. FT-IR and 1H-NMR analyses showed that the purified fraction was composed mainly of tricin-type flavonoids. The extracted tricin had strong scavenging capacity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals, with a half maximal inhibitory concentration (IC50) of 51.9 mg/L. Drug sensitivity paper testing showed that the extracted tricin inhibited the growth of Escherichia coli. The bacteriostatic circle diameters of tricin from wheat straw MWL and quercetin standard were 9.17 and 7.52 mm, respectively. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the tricin from wheat straw MWL had possible inhibitory effect on lung cancer A549 cells, with a maximum inhibitory performance of 44.5%.https://ojs.cnr.ncsu.edu/index.php/BRJ/article/view/22812tricinmwlthioacidolysisradicals scavenging antibacterialanticancer |
| spellingShingle | Yunbo Zhao Junyi Zhou Hujun Niu Yimin Xie Elucidation of Bioactivity of Tricin Released by Thioacidolysis from Wheat Straw Lignin BioResources tricin mwl thioacidolysis radicals scavenging antibacterial anticancer |
| title | Elucidation of Bioactivity of Tricin Released by Thioacidolysis from Wheat Straw Lignin |
| title_full | Elucidation of Bioactivity of Tricin Released by Thioacidolysis from Wheat Straw Lignin |
| title_fullStr | Elucidation of Bioactivity of Tricin Released by Thioacidolysis from Wheat Straw Lignin |
| title_full_unstemmed | Elucidation of Bioactivity of Tricin Released by Thioacidolysis from Wheat Straw Lignin |
| title_short | Elucidation of Bioactivity of Tricin Released by Thioacidolysis from Wheat Straw Lignin |
| title_sort | elucidation of bioactivity of tricin released by thioacidolysis from wheat straw lignin |
| topic | tricin mwl thioacidolysis radicals scavenging antibacterial anticancer |
| url | https://ojs.cnr.ncsu.edu/index.php/BRJ/article/view/22812 |
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