Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants
African swine fever (ASF) is a devastating disease of domestic pigs. It is a socioeconomically important disease, initially described from Kenya, but subsequently reported in most Sub-Saharan countries. ASF spread to Europe, South America and the Caribbean through multiple introductions which were i...
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MDPI AG
2017-02-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/9/2/31 |
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| author | Leopold K. Mulumba–Mfumu Jenna E. Achenbach Matthew R. Mauldin Linda K. Dixon Curé Georges Tshilenge Etienne Thiry Noelia Moreno Esther Blanco Claude Saegerman Charles E. Lamien Adama Diallo |
| author_facet | Leopold K. Mulumba–Mfumu Jenna E. Achenbach Matthew R. Mauldin Linda K. Dixon Curé Georges Tshilenge Etienne Thiry Noelia Moreno Esther Blanco Claude Saegerman Charles E. Lamien Adama Diallo |
| author_sort | Leopold K. Mulumba–Mfumu |
| collection | DOAJ |
| description | African swine fever (ASF) is a devastating disease of domestic pigs. It is a socioeconomically important disease, initially described from Kenya, but subsequently reported in most Sub-Saharan countries. ASF spread to Europe, South America and the Caribbean through multiple introductions which were initially eradicated—except for Sardinia—followed by re‑introduction into Europe in 2007. In this study of ASF within the Democratic Republic of the Congo, 62 domestic pig samples, collected between 2005–2012, were examined for viral DNA and sequencing at multiple loci: C-terminus of the B646L gene (p72 protein), central hypervariable region (CVR) of the B602L gene, and the E183L gene (p54 protein). Phylogenetic analyses identified three circulating genotypes: I (64.5% of samples), IX (32.3%), and XIV (3.2%). This is the first evidence of genotypes IX and XIV within this country. Examination of the CVR revealed high levels of intra-genotypic variation, with 19 identified variants. |
| first_indexed | 2024-04-12T14:41:44Z |
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| id | doaj.art-9e588d394e05463293df4725d1659cc5 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-04-12T14:41:44Z |
| publishDate | 2017-02-01 |
| publisher | MDPI AG |
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| series | Viruses |
| spelling | doaj.art-9e588d394e05463293df4725d1659cc52022-12-22T03:28:49ZengMDPI AGViruses1999-49152017-02-01923110.3390/v9020031v9020031Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 VariantsLeopold K. Mulumba–Mfumu0Jenna E. Achenbach1Matthew R. Mauldin2Linda K. Dixon3Curé Georges Tshilenge4Etienne Thiry5Noelia Moreno6Esther Blanco7Claude Saegerman8Charles E. Lamien9Adama Diallo10Central Veterinary Laboratory, Avenue Wangata, P.O. Box 8842, Kinshasa I, Democratic Republic of CongoAnimal Production and Health Laboratory, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, A-1400 Vienna, AustriaCenters for Disease Control and Prevention, Atlanta, GA 30333, USAPirbright Institute, Ash Road, Pirbright, Woking, Surrey GU24 ONF, UKCentral Veterinary Laboratory, Avenue Wangata, P.O. Box 8842, Kinshasa I, Democratic Republic of CongoResearch Unit of Epidemiology and Risk Analysis Applied to Veterinary (UREAR-Ulg), Fundamental and Applied Research for Animals & Health, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, BelgiumCentro de Investigaciόn en Sanidad Animal (CISA), INIA, Valdeolmos, 28130 Madrid, SpainCentro de Investigaciόn en Sanidad Animal (CISA), INIA, Valdeolmos, 28130 Madrid, SpainResearch Unit of Epidemiology and Risk Analysis Applied to Veterinary (UREAR-Ulg), Fundamental and Applied Research for Animals & Health, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, BelgiumAnimal Production and Health Laboratory, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, A-1400 Vienna, AustriaAnimal Production and Health Laboratory, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, A-1400 Vienna, AustriaAfrican swine fever (ASF) is a devastating disease of domestic pigs. It is a socioeconomically important disease, initially described from Kenya, but subsequently reported in most Sub-Saharan countries. ASF spread to Europe, South America and the Caribbean through multiple introductions which were initially eradicated—except for Sardinia—followed by re‑introduction into Europe in 2007. In this study of ASF within the Democratic Republic of the Congo, 62 domestic pig samples, collected between 2005–2012, were examined for viral DNA and sequencing at multiple loci: C-terminus of the B646L gene (p72 protein), central hypervariable region (CVR) of the B602L gene, and the E183L gene (p54 protein). Phylogenetic analyses identified three circulating genotypes: I (64.5% of samples), IX (32.3%), and XIV (3.2%). This is the first evidence of genotypes IX and XIV within this country. Examination of the CVR revealed high levels of intra-genotypic variation, with 19 identified variants.http://www.mdpi.com/1999-4915/9/2/31African swine fever virusoutbreaksDemocratic Republic of Congoswinegenotypesmolecular epidemiologyp72 genep54 geneCVR |
| spellingShingle | Leopold K. Mulumba–Mfumu Jenna E. Achenbach Matthew R. Mauldin Linda K. Dixon Curé Georges Tshilenge Etienne Thiry Noelia Moreno Esther Blanco Claude Saegerman Charles E. Lamien Adama Diallo Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants Viruses African swine fever virus outbreaks Democratic Republic of Congo swine genotypes molecular epidemiology p72 gene p54 gene CVR |
| title | Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants |
| title_full | Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants |
| title_fullStr | Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants |
| title_full_unstemmed | Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants |
| title_short | Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants |
| title_sort | genetic assessment of african swine fever isolates involved in outbreaks in the democratic republic of congo between 2005 and 2012 reveals co circulation of p72 genotypes i ix and xiv including 19 variants |
| topic | African swine fever virus outbreaks Democratic Republic of Congo swine genotypes molecular epidemiology p72 gene p54 gene CVR |
| url | http://www.mdpi.com/1999-4915/9/2/31 |
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