Extracorporeal Photopheresis Reduces Fibrotic and Inflammatory Transcriptomic Biological Marker of Chronic Antibody-mediated Kidney Rejection
Background. The benefit of extracorporeal photopheresis on the course of kidney transplant rejection is unknown. The aim of our study was to investigate the variations in transcriptomics on graft biopsies when extracorporeal photopheresis was used to treat chronic humoral rejection after kidney tran...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer
2024-03-01
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| Series: | Transplantation Direct |
| Online Access: | http://journals.lww.com/transplantationdirect/fulltext/10.1097/TXD.0000000000001587 |
| _version_ | 1797292768494616576 |
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| author | Arnaud Lionet, MD Marine Van Triempon, MD Martin Figeac, MD, PhD Victor Fages, MD Jean-Baptiste Gibier, MD, PhD François Provot, MD Mehdi Maanaoui, MD Nicolas Pottier, PharmD, PhD Christelle Cauffiez, PhD François Glowacki, MD, PhD |
| author_facet | Arnaud Lionet, MD Marine Van Triempon, MD Martin Figeac, MD, PhD Victor Fages, MD Jean-Baptiste Gibier, MD, PhD François Provot, MD Mehdi Maanaoui, MD Nicolas Pottier, PharmD, PhD Christelle Cauffiez, PhD François Glowacki, MD, PhD |
| author_sort | Arnaud Lionet, MD |
| collection | DOAJ |
| description | Background. The benefit of extracorporeal photopheresis on the course of kidney transplant rejection is unknown. The aim of our study was to investigate the variations in transcriptomics on graft biopsies when extracorporeal photopheresis was used to treat chronic humoral rejection after kidney transplantation.
Methods. We retrospectively analyzed the mRNA expression of 770 genes of interest in graft biopsies performed before and after treatment. Eight patients received an average of 23 extracorporeal photopheresis sessions over 4 mo between the 2 biopsies.
Results. Transcriptomic analysis of the graft biopsies identified a significant (adjusted P < 0.05) increase in CAV1 mRNA in all patients and a significant decrease in CD19, IL21, PAX5, and SFTPA2 mRNAs in 7 of 8 patients.
Conclusions. In patients treated with extracorporeal photopheresis for chronic humoral rejection after renal transplantation, omic analysis of repeated biopsies shows a reduction in fibrotic and inflammatory transcriptomic biologicals markers. |
| first_indexed | 2024-03-07T20:01:20Z |
| format | Article |
| id | doaj.art-9e592be6fbe44855a34e5956f81a2ecd |
| institution | Directory Open Access Journal |
| issn | 2373-8731 |
| language | English |
| last_indexed | 2024-03-07T20:01:20Z |
| publishDate | 2024-03-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Transplantation Direct |
| spelling | doaj.art-9e592be6fbe44855a34e5956f81a2ecd2024-02-28T06:50:07ZengWolters KluwerTransplantation Direct2373-87312024-03-01103e158710.1097/TXD.0000000000001587202403000-00006Extracorporeal Photopheresis Reduces Fibrotic and Inflammatory Transcriptomic Biological Marker of Chronic Antibody-mediated Kidney RejectionArnaud Lionet, MD0Marine Van Triempon, MD1Martin Figeac, MD, PhD2Victor Fages, MD3Jean-Baptiste Gibier, MD, PhD4François Provot, MD5Mehdi Maanaoui, MD6Nicolas Pottier, PharmD, PhD7Christelle Cauffiez, PhD8François Glowacki, MD, PhD91 Department of Nephrology and Kidney Transplantation, University Hospital Huriez, CHU-Lille, Lille, France.1 Department of Nephrology and Kidney Transplantation, University Hospital Huriez, CHU-Lille, Lille, France.3 CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, US 41—UAR 2014—PLBS, University of Lille, Lille, France.1 Department of Nephrology and Kidney Transplantation, University Hospital Huriez, CHU-Lille, Lille, France.4 Pathology Departement, CHU-Lille, France.1 Department of Nephrology and Kidney Transplantation, University Hospital Huriez, CHU-Lille, Lille, France.1 Department of Nephrology and Kidney Transplantation, University Hospital Huriez, CHU-Lille, Lille, France.2 CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, UMR9020-U1277—CANTHER, University of Lille, Lille, France.2 CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, UMR9020-U1277—CANTHER, University of Lille, Lille, France.1 Department of Nephrology and Kidney Transplantation, University Hospital Huriez, CHU-Lille, Lille, France.Background. The benefit of extracorporeal photopheresis on the course of kidney transplant rejection is unknown. The aim of our study was to investigate the variations in transcriptomics on graft biopsies when extracorporeal photopheresis was used to treat chronic humoral rejection after kidney transplantation. Methods. We retrospectively analyzed the mRNA expression of 770 genes of interest in graft biopsies performed before and after treatment. Eight patients received an average of 23 extracorporeal photopheresis sessions over 4 mo between the 2 biopsies. Results. Transcriptomic analysis of the graft biopsies identified a significant (adjusted P < 0.05) increase in CAV1 mRNA in all patients and a significant decrease in CD19, IL21, PAX5, and SFTPA2 mRNAs in 7 of 8 patients. Conclusions. In patients treated with extracorporeal photopheresis for chronic humoral rejection after renal transplantation, omic analysis of repeated biopsies shows a reduction in fibrotic and inflammatory transcriptomic biologicals markers.http://journals.lww.com/transplantationdirect/fulltext/10.1097/TXD.0000000000001587 |
| spellingShingle | Arnaud Lionet, MD Marine Van Triempon, MD Martin Figeac, MD, PhD Victor Fages, MD Jean-Baptiste Gibier, MD, PhD François Provot, MD Mehdi Maanaoui, MD Nicolas Pottier, PharmD, PhD Christelle Cauffiez, PhD François Glowacki, MD, PhD Extracorporeal Photopheresis Reduces Fibrotic and Inflammatory Transcriptomic Biological Marker of Chronic Antibody-mediated Kidney Rejection Transplantation Direct |
| title | Extracorporeal Photopheresis Reduces Fibrotic and Inflammatory Transcriptomic Biological Marker of Chronic Antibody-mediated Kidney Rejection |
| title_full | Extracorporeal Photopheresis Reduces Fibrotic and Inflammatory Transcriptomic Biological Marker of Chronic Antibody-mediated Kidney Rejection |
| title_fullStr | Extracorporeal Photopheresis Reduces Fibrotic and Inflammatory Transcriptomic Biological Marker of Chronic Antibody-mediated Kidney Rejection |
| title_full_unstemmed | Extracorporeal Photopheresis Reduces Fibrotic and Inflammatory Transcriptomic Biological Marker of Chronic Antibody-mediated Kidney Rejection |
| title_short | Extracorporeal Photopheresis Reduces Fibrotic and Inflammatory Transcriptomic Biological Marker of Chronic Antibody-mediated Kidney Rejection |
| title_sort | extracorporeal photopheresis reduces fibrotic and inflammatory transcriptomic biological marker of chronic antibody mediated kidney rejection |
| url | http://journals.lww.com/transplantationdirect/fulltext/10.1097/TXD.0000000000001587 |
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