Flower buds like PVA/ZnO composite nanofibers assembly: Antibacterial, in vivo wound healing, cytotoxicity and histological studies
Poly vinyl alcohol (PVA) nanofibers incorporated with ZnO nanoparticles were successfully fabricated by electrospinning technique. The developed nanofibrous composites were characterized by structural and morphological analysis techniques including X-ray diffraction (XRD), fourier-transform infrared...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-01-01
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| Series: | Polymer Testing |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0142941820321437 |
| _version_ | 1818568646974242816 |
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| author | Mohammad Ali Norouzi Majid Montazer Tina Harifi Pegah Karimi |
| author_facet | Mohammad Ali Norouzi Majid Montazer Tina Harifi Pegah Karimi |
| author_sort | Mohammad Ali Norouzi |
| collection | DOAJ |
| description | Poly vinyl alcohol (PVA) nanofibers incorporated with ZnO nanoparticles were successfully fabricated by electrospinning technique. The developed nanofibrous composites were characterized by structural and morphological analysis techniques including X-ray diffraction (XRD), fourier-transform infrared spectroscopy (FTIR), and field emission scanning electron microscopy (FESEM). Good dispersion of the nanoparticles in the resultant nanofibers was observed and the incorporated nanoparticles of ZnO were in the shape of flower buds. Moreover, porosity and swelling properties of the prepared membranes were evaluated. Antibacterial, cytotoxicity assays as well as in-vivo wound healing experiments in mice were also studied. The minimum inhibitory concentration (MIC) of PVA/ZnO nanoparticles against S. aureus and E. coli was 250 and 62.5 μg/mL respectively, indicating the antibacterial activities of the nanocomposite fibers relating to the zinc oxide nanoparticles. In-vivo studies and higher values of transforming growth factor-β (TGF-β) further proved that PVA/ZnO composite nanofibers had accelerated wound healing properties. ZnO nanoparticles were responsible for the accelerated epithelial regeneration and better cell attachment based on the histological analysis. |
| first_indexed | 2024-12-14T06:37:59Z |
| format | Article |
| id | doaj.art-9e59cc39f0174dd682067eb8ff9d49b6 |
| institution | Directory Open Access Journal |
| issn | 0142-9418 |
| language | English |
| last_indexed | 2024-12-14T06:37:59Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Polymer Testing |
| spelling | doaj.art-9e59cc39f0174dd682067eb8ff9d49b62022-12-21T23:13:20ZengElsevierPolymer Testing0142-94182021-01-0193106914Flower buds like PVA/ZnO composite nanofibers assembly: Antibacterial, in vivo wound healing, cytotoxicity and histological studiesMohammad Ali Norouzi0Majid Montazer1Tina Harifi2Pegah Karimi3Textile Department, South Tehran Branch, Islamic Azad University, Tehran, IranTextile Engineering Department, Functional Fibrous Structures & Environmental Enhancement (FFSEE), Amirkabir Nanotechnology Research Institute (ANTRI), Amirkabir University of Technology, Tehran, Iran; Corresponding author.Textile Engineering Department, Functional Fibrous Structures & Environmental Enhancement (FFSEE), Amirkabir University of Technology, Tehran, IranDepartment of Biochemistry, Faculty of Basic Sciences, Islamic Azad University, Central Tehran Brach, Tehran, IranPoly vinyl alcohol (PVA) nanofibers incorporated with ZnO nanoparticles were successfully fabricated by electrospinning technique. The developed nanofibrous composites were characterized by structural and morphological analysis techniques including X-ray diffraction (XRD), fourier-transform infrared spectroscopy (FTIR), and field emission scanning electron microscopy (FESEM). Good dispersion of the nanoparticles in the resultant nanofibers was observed and the incorporated nanoparticles of ZnO were in the shape of flower buds. Moreover, porosity and swelling properties of the prepared membranes were evaluated. Antibacterial, cytotoxicity assays as well as in-vivo wound healing experiments in mice were also studied. The minimum inhibitory concentration (MIC) of PVA/ZnO nanoparticles against S. aureus and E. coli was 250 and 62.5 μg/mL respectively, indicating the antibacterial activities of the nanocomposite fibers relating to the zinc oxide nanoparticles. In-vivo studies and higher values of transforming growth factor-β (TGF-β) further proved that PVA/ZnO composite nanofibers had accelerated wound healing properties. ZnO nanoparticles were responsible for the accelerated epithelial regeneration and better cell attachment based on the histological analysis.http://www.sciencedirect.com/science/article/pii/S0142941820321437PVA/ZnO electrospun NanofibersAntibacterialCytotoxicityWound healingHistological analysis |
| spellingShingle | Mohammad Ali Norouzi Majid Montazer Tina Harifi Pegah Karimi Flower buds like PVA/ZnO composite nanofibers assembly: Antibacterial, in vivo wound healing, cytotoxicity and histological studies Polymer Testing PVA/ZnO electrospun Nanofibers Antibacterial Cytotoxicity Wound healing Histological analysis |
| title | Flower buds like PVA/ZnO composite nanofibers assembly: Antibacterial, in vivo wound healing, cytotoxicity and histological studies |
| title_full | Flower buds like PVA/ZnO composite nanofibers assembly: Antibacterial, in vivo wound healing, cytotoxicity and histological studies |
| title_fullStr | Flower buds like PVA/ZnO composite nanofibers assembly: Antibacterial, in vivo wound healing, cytotoxicity and histological studies |
| title_full_unstemmed | Flower buds like PVA/ZnO composite nanofibers assembly: Antibacterial, in vivo wound healing, cytotoxicity and histological studies |
| title_short | Flower buds like PVA/ZnO composite nanofibers assembly: Antibacterial, in vivo wound healing, cytotoxicity and histological studies |
| title_sort | flower buds like pva zno composite nanofibers assembly antibacterial in vivo wound healing cytotoxicity and histological studies |
| topic | PVA/ZnO electrospun Nanofibers Antibacterial Cytotoxicity Wound healing Histological analysis |
| url | http://www.sciencedirect.com/science/article/pii/S0142941820321437 |
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