Decreased Expression of Cytotoxic Proteins in Decidual CD8<sup>+</sup> T Cells in Preeclampsia
In our study, we aimed to establish expression of cytotoxic CD8<sup>+</sup> T cells in the decidua basalis and the maternal peripheral blood (mPBL) of severe and mild preeclampsia (PE) and compare to healthy pregnancies. Decidual tissue and mPBL of 10 women with mild PE, 10 women with se...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Online Access: | https://www.mdpi.com/2079-7737/10/10/1037 |
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| author | Violeta Soljic Maja Barbaric Martina Vukoja Marina Curlin Martina Orlovic Vlaho Edita Cerni Obrdalj Lidija Lasic Arapovic Daniela Bevanda Glibo Katarina Vukojevic |
| author_facet | Violeta Soljic Maja Barbaric Martina Vukoja Marina Curlin Martina Orlovic Vlaho Edita Cerni Obrdalj Lidija Lasic Arapovic Daniela Bevanda Glibo Katarina Vukojevic |
| author_sort | Violeta Soljic |
| collection | DOAJ |
| description | In our study, we aimed to establish expression of cytotoxic CD8<sup>+</sup> T cells in the decidua basalis and the maternal peripheral blood (mPBL) of severe and mild preeclampsia (PE) and compare to healthy pregnancies. Decidual tissue and mPBL of 10 women with mild PE, 10 women with severe PE, and 20 age-matched healthy pregnancy controls were analyzed by double immunofluorescence and qPCR, respectively. By double immunofluorescence staining, we found a decreased total number of cells/mm<sup>2</sup> in decidua basalis of granulysin (GNLY)<sup>+</sup> (<i>p</i> ˂ 0.0001), granzyme B (GzB)<sup>+</sup>(<i>p</i> ˂ 0.0001), GzB<sup>+</sup>CD8<sup>+</sup>(<i>p</i> ˂ 0.0001), perforin (PRF1)<sup>+</sup> (<i>p</i> ˂ 0.0001), and PRF1<sup>+</sup>CD8<sup>+</sup> (<i>p</i> ˂ 0.01) in the severe PE compared to control group. Additionally, we noticed the trend of lower mRNA expression for GNLY, granzyme A (GZMA), GzB, and PRF1 in CD8<sup>+</sup> T cells of mPBL in mild and severe PE, with the latter marker statistically decreased in severe PE (<i>p</i> ˂ 0.001). Forkhead box P3 (FOXP3) mRNA in CD8<sup>+</sup> T cells mPBL was increased in mild PE (<i>p</i> ˂ 0.001) compared to controls. In conclusion, severe PE is characterized by altered expression of cytotoxic CD8<sup>+</sup> T cells in decidua and mPBL, suggesting their role in pathophysiology of PE and fetal-maternal immune tolerance. |
| first_indexed | 2024-03-10T06:43:37Z |
| format | Article |
| id | doaj.art-9e5bd697052f4ea6ac266a4bcbeeeb05 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T06:43:37Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-9e5bd697052f4ea6ac266a4bcbeeeb052023-11-22T17:28:53ZengMDPI AGBiology2079-77372021-10-011010103710.3390/biology10101037Decreased Expression of Cytotoxic Proteins in Decidual CD8<sup>+</sup> T Cells in PreeclampsiaVioleta Soljic0Maja Barbaric1Martina Vukoja2Marina Curlin3Martina Orlovic Vlaho4Edita Cerni Obrdalj5Lidija Lasic Arapovic6Daniela Bevanda Glibo7Katarina Vukojevic8Laboratory of Morphology, Department of Histology and Embryology, School of Medicine, University of Mostar, 88 000 Mostar, Bosnia and HerzegovinaLaboratory of Morphology, Department of Histology and Embryology, School of Medicine, University of Mostar, 88 000 Mostar, Bosnia and HerzegovinaLaboratory of Morphology, Department of Histology and Embryology, School of Medicine, University of Mostar, 88 000 Mostar, Bosnia and HerzegovinaFaculty of Health Studies, University of Mostar, 88 000 Mostar, Bosnia and HerzegovinaFaculty of Health Studies, University of Mostar, 88 000 Mostar, Bosnia and HerzegovinaDepartment of Family Medicine, School of Medicine, University of Mostar, 88 000 Mostar, Bosnia and HerzegovinaLaboratory of Morphology, Department of Histology and Embryology, School of Medicine, University of Mostar, 88 000 Mostar, Bosnia and HerzegovinaDepartment of Gastroenterology, University Hospital Mostar, 88000 Mostar, Bosnia and HerzegovinaLaboratory of Morphology, Department of Histology and Embryology, School of Medicine, University of Mostar, 88 000 Mostar, Bosnia and HerzegovinaIn our study, we aimed to establish expression of cytotoxic CD8<sup>+</sup> T cells in the decidua basalis and the maternal peripheral blood (mPBL) of severe and mild preeclampsia (PE) and compare to healthy pregnancies. Decidual tissue and mPBL of 10 women with mild PE, 10 women with severe PE, and 20 age-matched healthy pregnancy controls were analyzed by double immunofluorescence and qPCR, respectively. By double immunofluorescence staining, we found a decreased total number of cells/mm<sup>2</sup> in decidua basalis of granulysin (GNLY)<sup>+</sup> (<i>p</i> ˂ 0.0001), granzyme B (GzB)<sup>+</sup>(<i>p</i> ˂ 0.0001), GzB<sup>+</sup>CD8<sup>+</sup>(<i>p</i> ˂ 0.0001), perforin (PRF1)<sup>+</sup> (<i>p</i> ˂ 0.0001), and PRF1<sup>+</sup>CD8<sup>+</sup> (<i>p</i> ˂ 0.01) in the severe PE compared to control group. Additionally, we noticed the trend of lower mRNA expression for GNLY, granzyme A (GZMA), GzB, and PRF1 in CD8<sup>+</sup> T cells of mPBL in mild and severe PE, with the latter marker statistically decreased in severe PE (<i>p</i> ˂ 0.001). Forkhead box P3 (FOXP3) mRNA in CD8<sup>+</sup> T cells mPBL was increased in mild PE (<i>p</i> ˂ 0.001) compared to controls. In conclusion, severe PE is characterized by altered expression of cytotoxic CD8<sup>+</sup> T cells in decidua and mPBL, suggesting their role in pathophysiology of PE and fetal-maternal immune tolerance.https://www.mdpi.com/2079-7737/10/10/1037preeclampsiaperforingranulysingranzyme Agranzyme BFOXP3 |
| spellingShingle | Violeta Soljic Maja Barbaric Martina Vukoja Marina Curlin Martina Orlovic Vlaho Edita Cerni Obrdalj Lidija Lasic Arapovic Daniela Bevanda Glibo Katarina Vukojevic Decreased Expression of Cytotoxic Proteins in Decidual CD8<sup>+</sup> T Cells in Preeclampsia Biology preeclampsia perforin granulysin granzyme A granzyme B FOXP3 |
| title | Decreased Expression of Cytotoxic Proteins in Decidual CD8<sup>+</sup> T Cells in Preeclampsia |
| title_full | Decreased Expression of Cytotoxic Proteins in Decidual CD8<sup>+</sup> T Cells in Preeclampsia |
| title_fullStr | Decreased Expression of Cytotoxic Proteins in Decidual CD8<sup>+</sup> T Cells in Preeclampsia |
| title_full_unstemmed | Decreased Expression of Cytotoxic Proteins in Decidual CD8<sup>+</sup> T Cells in Preeclampsia |
| title_short | Decreased Expression of Cytotoxic Proteins in Decidual CD8<sup>+</sup> T Cells in Preeclampsia |
| title_sort | decreased expression of cytotoxic proteins in decidual cd8 sup sup t cells in preeclampsia |
| topic | preeclampsia perforin granulysin granzyme A granzyme B FOXP3 |
| url | https://www.mdpi.com/2079-7737/10/10/1037 |
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