Development of detection method for carcinogenic compounds using radish seeds (Raphanus sativus L.) via measuring the alkaline phosphatase enzyme activity

Convincing data regarding carcinogenic substances is very useful for handling materials correctly to avoid exposure to cancer-triggering substances in the body. To collect as much carcinogen data as possible, low-requirement carcinogen detection setups need to be developed. In this study, the first...

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Main Authors: Nuri Ari Efiana, Mulyadi, Annas Binarjo
Format: Article
Language:English
Published: Universitas Ahmad Dahlan 2023-11-01
Series:Pharmaciana
Subjects:
Online Access:http://journal.uad.ac.id/index.php/PHARMACIANA/article/view/27733/pdf_288
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author Nuri Ari Efiana
Mulyadi
Annas Binarjo
author_facet Nuri Ari Efiana
Mulyadi
Annas Binarjo
author_sort Nuri Ari Efiana
collection DOAJ
description Convincing data regarding carcinogenic substances is very useful for handling materials correctly to avoid exposure to cancer-triggering substances in the body. To collect as much carcinogen data as possible, low-requirement carcinogen detection setups need to be developed. In this study, the first steps in the development of carcinogen detection through functional protein expression assessment are reported. Hydrazine sulfate, one of the well-known carcinogens chosen as a model material, was tested on germination of Raphanus sativus L., with various concentrations, namely 0 (control), 1, 10, and 100 mM. This plant was selected due to its characteristics showing a stable germination rate over a wide range of temperatures. Within a predetermined germination time, seeds and/or sprouts were observed and germination rate, protein concentration, and alkaline phosphatase activity were measured. The results showed that hydrazine sulfate slowed seed germination, and increased 72-h protein concentration but decreased alkaline phosphatase activity in the control group. This suggests that changes in alkaline phosphatase activity in seeds during germination have the potential to be a marker for cancer. In this research, it can be concluded that the method used can be applied as a first step to assess the carcinogenicity of a compound.
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spelling doaj.art-9e5d7430f68249ec9df1174d78807d0a2024-02-26T02:03:24ZengUniversitas Ahmad DahlanPharmaciana2088-45592477-02562023-11-0113331732610.12928/pharmaciana.v13i3.27733Development of detection method for carcinogenic compounds using radish seeds (Raphanus sativus L.) via measuring the alkaline phosphatase enzyme activityNuri Ari Efiana0Mulyadi1Annas Binarjo2Faculty of Pharmacy, Universitas Ahmad DahlanBiochemical Laboratory, Faculty of Pharmacy, Universitas Gadjah MadaFaculty of Pharmacy, Universitas Ahmad DahlanConvincing data regarding carcinogenic substances is very useful for handling materials correctly to avoid exposure to cancer-triggering substances in the body. To collect as much carcinogen data as possible, low-requirement carcinogen detection setups need to be developed. In this study, the first steps in the development of carcinogen detection through functional protein expression assessment are reported. Hydrazine sulfate, one of the well-known carcinogens chosen as a model material, was tested on germination of Raphanus sativus L., with various concentrations, namely 0 (control), 1, 10, and 100 mM. This plant was selected due to its characteristics showing a stable germination rate over a wide range of temperatures. Within a predetermined germination time, seeds and/or sprouts were observed and germination rate, protein concentration, and alkaline phosphatase activity were measured. The results showed that hydrazine sulfate slowed seed germination, and increased 72-h protein concentration but decreased alkaline phosphatase activity in the control group. This suggests that changes in alkaline phosphatase activity in seeds during germination have the potential to be a marker for cancer. In this research, it can be concluded that the method used can be applied as a first step to assess the carcinogenicity of a compound.http://journal.uad.ac.id/index.php/PHARMACIANA/article/view/27733/pdf_288hydrazine sulfatecarcinogengerminationraphanus sativusalkaline phosphatase
spellingShingle Nuri Ari Efiana
Mulyadi
Annas Binarjo
Development of detection method for carcinogenic compounds using radish seeds (Raphanus sativus L.) via measuring the alkaline phosphatase enzyme activity
Pharmaciana
hydrazine sulfate
carcinogen
germination
raphanus sativus
alkaline phosphatase
title Development of detection method for carcinogenic compounds using radish seeds (Raphanus sativus L.) via measuring the alkaline phosphatase enzyme activity
title_full Development of detection method for carcinogenic compounds using radish seeds (Raphanus sativus L.) via measuring the alkaline phosphatase enzyme activity
title_fullStr Development of detection method for carcinogenic compounds using radish seeds (Raphanus sativus L.) via measuring the alkaline phosphatase enzyme activity
title_full_unstemmed Development of detection method for carcinogenic compounds using radish seeds (Raphanus sativus L.) via measuring the alkaline phosphatase enzyme activity
title_short Development of detection method for carcinogenic compounds using radish seeds (Raphanus sativus L.) via measuring the alkaline phosphatase enzyme activity
title_sort development of detection method for carcinogenic compounds using radish seeds raphanus sativus l via measuring the alkaline phosphatase enzyme activity
topic hydrazine sulfate
carcinogen
germination
raphanus sativus
alkaline phosphatase
url http://journal.uad.ac.id/index.php/PHARMACIANA/article/view/27733/pdf_288
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AT mulyadi developmentofdetectionmethodforcarcinogeniccompoundsusingradishseedsraphanussativuslviameasuringthealkalinephosphataseenzymeactivity
AT annasbinarjo developmentofdetectionmethodforcarcinogeniccompoundsusingradishseedsraphanussativuslviameasuringthealkalinephosphataseenzymeactivity