Impact of a Novel W2027L Mutation and Non-Target Site Resistance on Acetyl-CoA Carboxylase-Inhibiting Herbicides in a French <i>Lolium multiflorum</i> Population
Herbicides that inhibit acetyl-CoA carboxylase (ACCase) are among the few remaining options for the post-emergence control of <i>Lolium</i> species in small grain cereal crops. Here, we determined the mechanism of resistance to ACCase herbicides in a <i>Lolium multiflorum</i>...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Genes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4425/12/11/1838 |
| _version_ | 1797510165965045760 |
|---|---|
| author | Shiv Shankhar Kaundun Joe Downes Lucy Victoria Jackson Sarah-Jane Hutchings Eddie Mcindoe |
| author_facet | Shiv Shankhar Kaundun Joe Downes Lucy Victoria Jackson Sarah-Jane Hutchings Eddie Mcindoe |
| author_sort | Shiv Shankhar Kaundun |
| collection | DOAJ |
| description | Herbicides that inhibit acetyl-CoA carboxylase (ACCase) are among the few remaining options for the post-emergence control of <i>Lolium</i> species in small grain cereal crops. Here, we determined the mechanism of resistance to ACCase herbicides in a <i>Lolium multiflorum</i> population (HGR) from France. A combined biological and molecular approach detected a novel W2027L ACCase mutation that affects aryloxyphenoxypropionate (FOP) but not cyclohexanedione (DIM) or phenylpyraxoline (DEN) subclasses of ACCase herbicides. Both the wild-type tryptophan and mutant leucine 2027-ACCase alleles could be positively detected in a single DNA-based-derived polymorphic amplified cleaved sequence (dPACS) assay that contained the targeted PCR product and a cocktail of two discriminating restriction enzymes. Additionally, we identified three well-characterised I1781L, I2041T, and D2078G ACCase target site resistance mutations as well as non-target site resistance in HGR. The non-target site component endowed high levels of resistance to FOP herbicides whilst partially impacting on the efficacy of pinoxaden and cycloxydim. This study adequately assessed the contribution of the W2027L mutation and non-target site mechanism in conferring resistance to ACCase herbicides in HGR. It also highlights the versatility and robustness of the dPACS method to simultaneously identify different resistance-causing alleles at a single ACCase codon. |
| first_indexed | 2024-03-10T05:28:43Z |
| format | Article |
| id | doaj.art-9e5f6b6126f04755aa0e6fe01502718f |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T05:28:43Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-9e5f6b6126f04755aa0e6fe01502718f2023-11-22T23:29:32ZengMDPI AGGenes2073-44252021-11-011211183810.3390/genes12111838Impact of a Novel W2027L Mutation and Non-Target Site Resistance on Acetyl-CoA Carboxylase-Inhibiting Herbicides in a French <i>Lolium multiflorum</i> PopulationShiv Shankhar Kaundun0Joe Downes1Lucy Victoria Jackson2Sarah-Jane Hutchings3Eddie Mcindoe4Herbicide Bioscience, Jealott’s Hill International Research Centre, Syngenta, Bracknell RG42 6EY, UKHerbicide Bioscience, Jealott’s Hill International Research Centre, Syngenta, Bracknell RG42 6EY, UKHerbicide Bioscience, Jealott’s Hill International Research Centre, Syngenta, Bracknell RG42 6EY, UKHerbicide Bioscience, Jealott’s Hill International Research Centre, Syngenta, Bracknell RG42 6EY, UKHerbicide Bioscience, Jealott’s Hill International Research Centre, Syngenta, Bracknell RG42 6EY, UKHerbicides that inhibit acetyl-CoA carboxylase (ACCase) are among the few remaining options for the post-emergence control of <i>Lolium</i> species in small grain cereal crops. Here, we determined the mechanism of resistance to ACCase herbicides in a <i>Lolium multiflorum</i> population (HGR) from France. A combined biological and molecular approach detected a novel W2027L ACCase mutation that affects aryloxyphenoxypropionate (FOP) but not cyclohexanedione (DIM) or phenylpyraxoline (DEN) subclasses of ACCase herbicides. Both the wild-type tryptophan and mutant leucine 2027-ACCase alleles could be positively detected in a single DNA-based-derived polymorphic amplified cleaved sequence (dPACS) assay that contained the targeted PCR product and a cocktail of two discriminating restriction enzymes. Additionally, we identified three well-characterised I1781L, I2041T, and D2078G ACCase target site resistance mutations as well as non-target site resistance in HGR. The non-target site component endowed high levels of resistance to FOP herbicides whilst partially impacting on the efficacy of pinoxaden and cycloxydim. This study adequately assessed the contribution of the W2027L mutation and non-target site mechanism in conferring resistance to ACCase herbicides in HGR. It also highlights the versatility and robustness of the dPACS method to simultaneously identify different resistance-causing alleles at a single ACCase codon.https://www.mdpi.com/2073-4425/12/11/1838herbicideacetyl-CoA carboxylase<i>Lolium multiflorum</i>mechanism of resistanceI1781LI2041T |
| spellingShingle | Shiv Shankhar Kaundun Joe Downes Lucy Victoria Jackson Sarah-Jane Hutchings Eddie Mcindoe Impact of a Novel W2027L Mutation and Non-Target Site Resistance on Acetyl-CoA Carboxylase-Inhibiting Herbicides in a French <i>Lolium multiflorum</i> Population Genes herbicide acetyl-CoA carboxylase <i>Lolium multiflorum</i> mechanism of resistance I1781L I2041T |
| title | Impact of a Novel W2027L Mutation and Non-Target Site Resistance on Acetyl-CoA Carboxylase-Inhibiting Herbicides in a French <i>Lolium multiflorum</i> Population |
| title_full | Impact of a Novel W2027L Mutation and Non-Target Site Resistance on Acetyl-CoA Carboxylase-Inhibiting Herbicides in a French <i>Lolium multiflorum</i> Population |
| title_fullStr | Impact of a Novel W2027L Mutation and Non-Target Site Resistance on Acetyl-CoA Carboxylase-Inhibiting Herbicides in a French <i>Lolium multiflorum</i> Population |
| title_full_unstemmed | Impact of a Novel W2027L Mutation and Non-Target Site Resistance on Acetyl-CoA Carboxylase-Inhibiting Herbicides in a French <i>Lolium multiflorum</i> Population |
| title_short | Impact of a Novel W2027L Mutation and Non-Target Site Resistance on Acetyl-CoA Carboxylase-Inhibiting Herbicides in a French <i>Lolium multiflorum</i> Population |
| title_sort | impact of a novel w2027l mutation and non target site resistance on acetyl coa carboxylase inhibiting herbicides in a french i lolium multiflorum i population |
| topic | herbicide acetyl-CoA carboxylase <i>Lolium multiflorum</i> mechanism of resistance I1781L I2041T |
| url | https://www.mdpi.com/2073-4425/12/11/1838 |
| work_keys_str_mv | AT shivshankharkaundun impactofanovelw2027lmutationandnontargetsiteresistanceonacetylcoacarboxylaseinhibitingherbicidesinafrenchiloliummultiflorumipopulation AT joedownes impactofanovelw2027lmutationandnontargetsiteresistanceonacetylcoacarboxylaseinhibitingherbicidesinafrenchiloliummultiflorumipopulation AT lucyvictoriajackson impactofanovelw2027lmutationandnontargetsiteresistanceonacetylcoacarboxylaseinhibitingherbicidesinafrenchiloliummultiflorumipopulation AT sarahjanehutchings impactofanovelw2027lmutationandnontargetsiteresistanceonacetylcoacarboxylaseinhibitingherbicidesinafrenchiloliummultiflorumipopulation AT eddiemcindoe impactofanovelw2027lmutationandnontargetsiteresistanceonacetylcoacarboxylaseinhibitingherbicidesinafrenchiloliummultiflorumipopulation |