Isolation and characterization of a novel parvovirus from a red-crowned crane, China, 2021
Abstract Background Parvoviruses are icosahedral, nonenveloped viruses with single-stranded DNA genomes of approximately 5 kb in length. In recent years, parvoviruses have frequently mutated and expanded their host range to cause disease in many wild animals by altering their tissue tropism. Animal...
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-023-03683-4 |
| _version_ | 1797559036998057984 |
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| author | Hao Liu Jie Huang Zi-Shuo Lu Li-Xia Li Xiao-Tong Liang Tian Tang Wen-Chao Sun Hui-Jun Lu Ning-Yi Jin Xue Bai Xing-Kui Si |
| author_facet | Hao Liu Jie Huang Zi-Shuo Lu Li-Xia Li Xiao-Tong Liang Tian Tang Wen-Chao Sun Hui-Jun Lu Ning-Yi Jin Xue Bai Xing-Kui Si |
| author_sort | Hao Liu |
| collection | DOAJ |
| description | Abstract Background Parvoviruses are icosahedral, nonenveloped viruses with single-stranded DNA genomes of approximately 5 kb in length. In recent years, parvoviruses have frequently mutated and expanded their host range to cause disease in many wild animals by altering their tissue tropism. Animal infection mainly results in acute enteritis and inflammation of other organs. In this study, we used a viral metagenomic method to detect a novel parvovirus species in a red-crowned crane that died due to severe diarrhea in China. Results The presence of the viral genome in the kidney, lung, heart, liver, and intestine were confirmed by PCR. Histopathological examination of the intestine showed a large number of infiltrated inflammatory cells. The JL21/10 strain of the red-crowned crane parvovirus was first isolated from the intestine. Whole-genome sequence analysis showed that JL21/10 shared high identity with the red-crowned crane Parvovirinae strains yc-8 at the nucleotide level (96.61%). Phylogenetic analysis of the complete genome and NS1 gene revealed that the JL21/10 strain clustered with strains in chicken and revealed a close genetic relationship with the red-crowned crane parvovirus strains.The complete of VP2 gene analysis showed that JL21/10 shared identity with the red-crowned crane yc-8 strains (97.7%), chicken (55.4%),ducks(31.0%) and geese(30.1%) at the amino acid level. The result showed that red-crowned crane parvovirus may be cross-species transmission to chicken. However, There is little possibility of transmission to ducks and geese. Conclusion This is the first isolation and identification of a parvovirus in red-crowned crane that was associated with severe diarrhea. |
| first_indexed | 2024-03-10T17:40:03Z |
| format | Article |
| id | doaj.art-9e66d8beb949448d8610acb7b9c5cdc8 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-03-10T17:40:03Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-9e66d8beb949448d8610acb7b9c5cdc82023-11-20T09:45:10ZengBMCBMC Veterinary Research1746-61482023-09-011911610.1186/s12917-023-03683-4Isolation and characterization of a novel parvovirus from a red-crowned crane, China, 2021Hao Liu0Jie Huang1Zi-Shuo Lu2Li-Xia Li3Xiao-Tong Liang4Tian Tang5Wen-Chao Sun6Hui-Jun Lu7Ning-Yi Jin8Xue Bai9Xing-Kui Si10School of Life Sciences and Engineering, Foshan UniversitySchool of Life Sciences and Engineering, Foshan UniversitySchool of Life Sciences and Engineering, Foshan UniversitySchool of Life Sciences and Engineering, Foshan UniversitySchool of Life Sciences and Engineering, Foshan UniversitySchool of Life Sciences and Engineering, Foshan UniversityInstitute of Virology, Wenzhou UniversityInstitute of Military Veterinary, Academy of Military Medical SciencesInstitute of Military Veterinary, Academy of Military Medical SciencesInstitute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural SciencesSchool of Life Sciences and Engineering, Foshan UniversityAbstract Background Parvoviruses are icosahedral, nonenveloped viruses with single-stranded DNA genomes of approximately 5 kb in length. In recent years, parvoviruses have frequently mutated and expanded their host range to cause disease in many wild animals by altering their tissue tropism. Animal infection mainly results in acute enteritis and inflammation of other organs. In this study, we used a viral metagenomic method to detect a novel parvovirus species in a red-crowned crane that died due to severe diarrhea in China. Results The presence of the viral genome in the kidney, lung, heart, liver, and intestine were confirmed by PCR. Histopathological examination of the intestine showed a large number of infiltrated inflammatory cells. The JL21/10 strain of the red-crowned crane parvovirus was first isolated from the intestine. Whole-genome sequence analysis showed that JL21/10 shared high identity with the red-crowned crane Parvovirinae strains yc-8 at the nucleotide level (96.61%). Phylogenetic analysis of the complete genome and NS1 gene revealed that the JL21/10 strain clustered with strains in chicken and revealed a close genetic relationship with the red-crowned crane parvovirus strains.The complete of VP2 gene analysis showed that JL21/10 shared identity with the red-crowned crane yc-8 strains (97.7%), chicken (55.4%),ducks(31.0%) and geese(30.1%) at the amino acid level. The result showed that red-crowned crane parvovirus may be cross-species transmission to chicken. However, There is little possibility of transmission to ducks and geese. Conclusion This is the first isolation and identification of a parvovirus in red-crowned crane that was associated with severe diarrhea.https://doi.org/10.1186/s12917-023-03683-4EnterovirusRed-crowned craneParvovirusDetectionGenome analysisPhylogeny |
| spellingShingle | Hao Liu Jie Huang Zi-Shuo Lu Li-Xia Li Xiao-Tong Liang Tian Tang Wen-Chao Sun Hui-Jun Lu Ning-Yi Jin Xue Bai Xing-Kui Si Isolation and characterization of a novel parvovirus from a red-crowned crane, China, 2021 BMC Veterinary Research Enterovirus Red-crowned crane Parvovirus Detection Genome analysis Phylogeny |
| title | Isolation and characterization of a novel parvovirus from a red-crowned crane, China, 2021 |
| title_full | Isolation and characterization of a novel parvovirus from a red-crowned crane, China, 2021 |
| title_fullStr | Isolation and characterization of a novel parvovirus from a red-crowned crane, China, 2021 |
| title_full_unstemmed | Isolation and characterization of a novel parvovirus from a red-crowned crane, China, 2021 |
| title_short | Isolation and characterization of a novel parvovirus from a red-crowned crane, China, 2021 |
| title_sort | isolation and characterization of a novel parvovirus from a red crowned crane china 2021 |
| topic | Enterovirus Red-crowned crane Parvovirus Detection Genome analysis Phylogeny |
| url | https://doi.org/10.1186/s12917-023-03683-4 |
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