Dual RNA-Seq of Trunk Kidneys Extracted From Channel Catfish Infected With Yersinia ruckeri Reveals Novel Insights Into Host-Pathogen Interactions
Host-pathogen intectarions are complex, involving large dynamic changes in gene expression through the process of infection. These interactions are essential for understanding anti-infective immunity as well as pathogenesis. In this study, the host-pathogen interaction was analyzed using a model of...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.775708/full |
| _version_ | 1811222552060624896 |
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| author | Yibin Yang Yibin Yang Xia Zhu Haixin Zhang Yuhua Chen Yuhua Chen Yi Song Xiaohui Ai Xiaohui Ai |
| author_facet | Yibin Yang Yibin Yang Xia Zhu Haixin Zhang Yuhua Chen Yuhua Chen Yi Song Xiaohui Ai Xiaohui Ai |
| author_sort | Yibin Yang |
| collection | DOAJ |
| description | Host-pathogen intectarions are complex, involving large dynamic changes in gene expression through the process of infection. These interactions are essential for understanding anti-infective immunity as well as pathogenesis. In this study, the host-pathogen interaction was analyzed using a model of acute infection where channel catfish were infected with Yersinia ruckeri. The infected fish showed signs of body surface hyperemia as well as hyperemia and swelling in the trunk kidney. Double RNA sequencing was performed on trunk kidneys extracted from infected channel catfish and transcriptome data was compared with data from uninfected trunk kidneys. Results revealed that the host-pathogen interaction was dynamically regulated and that the host-pathogen transcriptome fluctuated during infection. More specifically, these data revealed that the expression levels of immune genes involved in Cytokine-cytokine receptor interactions, the NF-kappa B signaling pathway, the JAK-STAT signaling pathway, Toll-like receptor signaling and other immune-related pathways were significantly upregulated. Y. ruckeri mainly promote pathogenesis through the flagellum gene fliC in channel catfish. The weighted gene co-expression network analysis (WGCNA) R package was used to reveal that the infection of catfish is closely related to metabolic pathways. This study contributes to the understanding of the host-pathogen interaction between channel catfish and Y. ruckeri, more specifically how catfish respond to infection through a transcriptional perspective and how this infection leads to enteric red mouth disease (ERM) in these fish. |
| first_indexed | 2024-04-12T08:17:52Z |
| format | Article |
| id | doaj.art-9e690fb31e2844aeb1dad3bbabbd891a |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-12T08:17:52Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-9e690fb31e2844aeb1dad3bbabbd891a2022-12-22T03:40:43ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-12-011210.3389/fimmu.2021.775708775708Dual RNA-Seq of Trunk Kidneys Extracted From Channel Catfish Infected With Yersinia ruckeri Reveals Novel Insights Into Host-Pathogen InteractionsYibin Yang0Yibin Yang1Xia Zhu2Haixin Zhang3Yuhua Chen4Yuhua Chen5Yi Song6Xiaohui Ai7Xiaohui Ai8Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, ChinaThe Key Laboratory for Quality and Safety Control of Aquatic Products, Ministry of Agriculture, Beijing, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, ChinaFish Disease Laboratory, Jiangxi Fisheries Research Institute, Nanchang, ChinaDepartment of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan, ChinaHubei Clinical Center & Key Lab of Intestinal & Colorectal Diseases, Zhongnan Hospital of Wuhan University, Wuhan, ChinaThe Key Laboratory for Quality and Safety Control of Aquatic Products, Ministry of Agriculture, Beijing, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, ChinaThe Key Laboratory for Quality and Safety Control of Aquatic Products, Ministry of Agriculture, Beijing, ChinaHost-pathogen intectarions are complex, involving large dynamic changes in gene expression through the process of infection. These interactions are essential for understanding anti-infective immunity as well as pathogenesis. In this study, the host-pathogen interaction was analyzed using a model of acute infection where channel catfish were infected with Yersinia ruckeri. The infected fish showed signs of body surface hyperemia as well as hyperemia and swelling in the trunk kidney. Double RNA sequencing was performed on trunk kidneys extracted from infected channel catfish and transcriptome data was compared with data from uninfected trunk kidneys. Results revealed that the host-pathogen interaction was dynamically regulated and that the host-pathogen transcriptome fluctuated during infection. More specifically, these data revealed that the expression levels of immune genes involved in Cytokine-cytokine receptor interactions, the NF-kappa B signaling pathway, the JAK-STAT signaling pathway, Toll-like receptor signaling and other immune-related pathways were significantly upregulated. Y. ruckeri mainly promote pathogenesis through the flagellum gene fliC in channel catfish. The weighted gene co-expression network analysis (WGCNA) R package was used to reveal that the infection of catfish is closely related to metabolic pathways. This study contributes to the understanding of the host-pathogen interaction between channel catfish and Y. ruckeri, more specifically how catfish respond to infection through a transcriptional perspective and how this infection leads to enteric red mouth disease (ERM) in these fish.https://www.frontiersin.org/articles/10.3389/fimmu.2021.775708/fullchannel catfishYersinia ruckeridual RNA-seqhost-pathogen interactionsimmunityvirulence |
| spellingShingle | Yibin Yang Yibin Yang Xia Zhu Haixin Zhang Yuhua Chen Yuhua Chen Yi Song Xiaohui Ai Xiaohui Ai Dual RNA-Seq of Trunk Kidneys Extracted From Channel Catfish Infected With Yersinia ruckeri Reveals Novel Insights Into Host-Pathogen Interactions Frontiers in Immunology channel catfish Yersinia ruckeri dual RNA-seq host-pathogen interactions immunity virulence |
| title | Dual RNA-Seq of Trunk Kidneys Extracted From Channel Catfish Infected With Yersinia ruckeri Reveals Novel Insights Into Host-Pathogen Interactions |
| title_full | Dual RNA-Seq of Trunk Kidneys Extracted From Channel Catfish Infected With Yersinia ruckeri Reveals Novel Insights Into Host-Pathogen Interactions |
| title_fullStr | Dual RNA-Seq of Trunk Kidneys Extracted From Channel Catfish Infected With Yersinia ruckeri Reveals Novel Insights Into Host-Pathogen Interactions |
| title_full_unstemmed | Dual RNA-Seq of Trunk Kidneys Extracted From Channel Catfish Infected With Yersinia ruckeri Reveals Novel Insights Into Host-Pathogen Interactions |
| title_short | Dual RNA-Seq of Trunk Kidneys Extracted From Channel Catfish Infected With Yersinia ruckeri Reveals Novel Insights Into Host-Pathogen Interactions |
| title_sort | dual rna seq of trunk kidneys extracted from channel catfish infected with yersinia ruckeri reveals novel insights into host pathogen interactions |
| topic | channel catfish Yersinia ruckeri dual RNA-seq host-pathogen interactions immunity virulence |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.775708/full |
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