Exploring the Effects of Robertsonian Translocation 1/29 (Rob (1;29)) on Genetic Diversity in Minor Breeds of Spanish Berrenda Cattle via Genome-Wide Analysis
Most of the previous studies on the genetic variability in Spanish “Berrenda” breeds have been carried out using DNA microsatellites. The present work aimed to estimate the genetic diversity, population structure, and potential genetic differences among individuals of both Berrenda breeds and groups...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-03-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/14/5/793 |
| _version_ | 1797264929680523264 |
|---|---|
| author | Rafael González-Cano Ana González-Martínez Manuel Ramón Miriam González Serrano Miguel Moreno Millán Alejandro Rubio de Juan Evangelina Rodero Serrano |
| author_facet | Rafael González-Cano Ana González-Martínez Manuel Ramón Miriam González Serrano Miguel Moreno Millán Alejandro Rubio de Juan Evangelina Rodero Serrano |
| author_sort | Rafael González-Cano |
| collection | DOAJ |
| description | Most of the previous studies on the genetic variability in Spanish “Berrenda” breeds have been carried out using DNA microsatellites. The present work aimed to estimate the genetic diversity, population structure, and potential genetic differences among individuals of both Berrenda breeds and groups based on the presence of the Robertsonian chromosomal translocation, rob (1;29). A total of 373 samples from animals belonging to the two breeds, including 169 cases diagnosed as rob (1;29)-positive, were genotyped using an SNP50K chip. The genetic diversity at the breed level did not show significant differences, but it was significantly lower in those subpopulations containing the rob (1;29). Runs of homozygosity identified a region of homozygosity on chromosome 6, where the <i>KIT</i> (<i>KIT</i> proto-oncogene, receptor tyrosine kinase) gene, which determines the typical spotted coat pattern in both breeds, is located. The four subpopulations considered showed minor genetic differences. The regions of the genome that most determined the differences between the breeds were observed on chromosomes 4, 6, 18, and 22. The presence of this Robertsonian translocation did not result in sub-structuring within each of the breeds considered. To improve the reproductive performance of Berrenda breeds, it would be necessary to implement strategies considering the involvement of potential breeding stock carrying rob (1;29). |
| first_indexed | 2024-04-25T00:36:43Z |
| format | Article |
| id | doaj.art-9e6a36b431814a72b7d76ec984735aff |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-04-25T00:36:43Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-9e6a36b431814a72b7d76ec984735aff2024-03-12T16:38:20ZengMDPI AGAnimals2076-26152024-03-0114579310.3390/ani14050793Exploring the Effects of Robertsonian Translocation 1/29 (Rob (1;29)) on Genetic Diversity in Minor Breeds of Spanish Berrenda Cattle via Genome-Wide AnalysisRafael González-Cano0Ana González-Martínez1Manuel Ramón2Miriam González Serrano3Miguel Moreno Millán4Alejandro Rubio de Juan5Evangelina Rodero Serrano6Ministry of Agriculture, Fisheries and Food, Paseo Infanta Isabel 1, 28014 Madrid, SpainDepartment of Animal Production, Faculty of Veterinary Sciences, University of Cordoba, 14071 Córdoba, SpainDepartment of Animal Breeding and Genetics, National Institute for Agricultural and Food Research and Technology (INIA-CSIC), 28040 Madrid, SpainDepartment of Animal Production, Faculty of Veterinary Sciences, University of Cordoba, 14071 Córdoba, SpainDepartment of Genetic, Faculty of Veterinary Sciences, University of Cordoba, 14071 Córdoba, SpainRegional Center of Animal Breeding and Reproduction (CERSYRA-IRIAF), Avenida del Vino 10, 13300 Ciudad Real, SpainDepartment of Animal Production, Faculty of Veterinary Sciences, University of Cordoba, 14071 Córdoba, SpainMost of the previous studies on the genetic variability in Spanish “Berrenda” breeds have been carried out using DNA microsatellites. The present work aimed to estimate the genetic diversity, population structure, and potential genetic differences among individuals of both Berrenda breeds and groups based on the presence of the Robertsonian chromosomal translocation, rob (1;29). A total of 373 samples from animals belonging to the two breeds, including 169 cases diagnosed as rob (1;29)-positive, were genotyped using an SNP50K chip. The genetic diversity at the breed level did not show significant differences, but it was significantly lower in those subpopulations containing the rob (1;29). Runs of homozygosity identified a region of homozygosity on chromosome 6, where the <i>KIT</i> (<i>KIT</i> proto-oncogene, receptor tyrosine kinase) gene, which determines the typical spotted coat pattern in both breeds, is located. The four subpopulations considered showed minor genetic differences. The regions of the genome that most determined the differences between the breeds were observed on chromosomes 4, 6, 18, and 22. The presence of this Robertsonian translocation did not result in sub-structuring within each of the breeds considered. To improve the reproductive performance of Berrenda breeds, it would be necessary to implement strategies considering the involvement of potential breeding stock carrying rob (1;29).https://www.mdpi.com/2076-2615/14/5/793beef cattlediversity conservationSNPcytogenetic analysisRobertsonian translocation |
| spellingShingle | Rafael González-Cano Ana González-Martínez Manuel Ramón Miriam González Serrano Miguel Moreno Millán Alejandro Rubio de Juan Evangelina Rodero Serrano Exploring the Effects of Robertsonian Translocation 1/29 (Rob (1;29)) on Genetic Diversity in Minor Breeds of Spanish Berrenda Cattle via Genome-Wide Analysis Animals beef cattle diversity conservation SNP cytogenetic analysis Robertsonian translocation |
| title | Exploring the Effects of Robertsonian Translocation 1/29 (Rob (1;29)) on Genetic Diversity in Minor Breeds of Spanish Berrenda Cattle via Genome-Wide Analysis |
| title_full | Exploring the Effects of Robertsonian Translocation 1/29 (Rob (1;29)) on Genetic Diversity in Minor Breeds of Spanish Berrenda Cattle via Genome-Wide Analysis |
| title_fullStr | Exploring the Effects of Robertsonian Translocation 1/29 (Rob (1;29)) on Genetic Diversity in Minor Breeds of Spanish Berrenda Cattle via Genome-Wide Analysis |
| title_full_unstemmed | Exploring the Effects of Robertsonian Translocation 1/29 (Rob (1;29)) on Genetic Diversity in Minor Breeds of Spanish Berrenda Cattle via Genome-Wide Analysis |
| title_short | Exploring the Effects of Robertsonian Translocation 1/29 (Rob (1;29)) on Genetic Diversity in Minor Breeds of Spanish Berrenda Cattle via Genome-Wide Analysis |
| title_sort | exploring the effects of robertsonian translocation 1 29 rob 1 29 on genetic diversity in minor breeds of spanish berrenda cattle via genome wide analysis |
| topic | beef cattle diversity conservation SNP cytogenetic analysis Robertsonian translocation |
| url | https://www.mdpi.com/2076-2615/14/5/793 |
| work_keys_str_mv | AT rafaelgonzalezcano exploringtheeffectsofrobertsoniantranslocation129rob129ongeneticdiversityinminorbreedsofspanishberrendacattleviagenomewideanalysis AT anagonzalezmartinez exploringtheeffectsofrobertsoniantranslocation129rob129ongeneticdiversityinminorbreedsofspanishberrendacattleviagenomewideanalysis AT manuelramon exploringtheeffectsofrobertsoniantranslocation129rob129ongeneticdiversityinminorbreedsofspanishberrendacattleviagenomewideanalysis AT miriamgonzalezserrano exploringtheeffectsofrobertsoniantranslocation129rob129ongeneticdiversityinminorbreedsofspanishberrendacattleviagenomewideanalysis AT miguelmorenomillan exploringtheeffectsofrobertsoniantranslocation129rob129ongeneticdiversityinminorbreedsofspanishberrendacattleviagenomewideanalysis AT alejandrorubiodejuan exploringtheeffectsofrobertsoniantranslocation129rob129ongeneticdiversityinminorbreedsofspanishberrendacattleviagenomewideanalysis AT evangelinaroderoserrano exploringtheeffectsofrobertsoniantranslocation129rob129ongeneticdiversityinminorbreedsofspanishberrendacattleviagenomewideanalysis |