Serotonin Transporter mRNA Expression Is Reduced in the Peripheral Blood Mononuclear Cells of Subjects with Major Depression but Normal in Fibromyalgia
Background: Fibromyalgia (FM) and major depression disorder (MDD) frequently co-occur. Both disorders may share common serotonergic alterations, although there is less evidence of such alterations in FM. It is also unclear as to whether these alterations are persistent over time or transient. The ob...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Brain Sciences |
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| Online Access: | https://www.mdpi.com/2076-3425/13/10/1485 |
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| author | Gaël Villanueva-Charbonneau Stéphane Potvin Serge Marchand Alexander McIntyre Diane McIntosh Alain Bissonnette Alain Gendron Charles-Édouard Giguère Marie-Ève Koué Édouard Kouassi |
| author_facet | Gaël Villanueva-Charbonneau Stéphane Potvin Serge Marchand Alexander McIntyre Diane McIntosh Alain Bissonnette Alain Gendron Charles-Édouard Giguère Marie-Ève Koué Édouard Kouassi |
| author_sort | Gaël Villanueva-Charbonneau |
| collection | DOAJ |
| description | Background: Fibromyalgia (FM) and major depression disorder (MDD) frequently co-occur. Both disorders may share common serotonergic alterations, although there is less evidence of such alterations in FM. It is also unclear as to whether these alterations are persistent over time or transient. The objectives of this study were to (i) examine the changes in mRNA expression of serotonin transporter (SERT) on the surface of peripheral blood mononuclear cells (PBMCs) in FM, MDD, and the FM + MDD subjects compared to healthy controls, and to (ii) evaluate the effect of drug treatment on SERT expression. Methods: PBMCs were isolated from FM, MDD, FM + MDD, and control subjects. SERT expression was analyzed at the mRNA level via quantitative real-time polymerase chain reaction. Statistical analyses were performed using analyses of variance and linear mixed-effects models. Results: SERT mRNA expression was significantly reduced in MDD subjects compared to controls (<i>p</i> < 0.001), but not in FM nor in FM + MDD subjects. Although the drug treatments improved symptoms in FM, MDD, and FM + MDD subjects, they had no significant effect on SERT mRNA expression. Conclusions: These results corroborate the role of the SERT in the pathophysiology of MDD, but not in FM, and show that the decreased mRNA expression of SERT is a persistent, rather than transient, phenomenon. |
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| language | English |
| last_indexed | 2024-03-10T21:23:43Z |
| publishDate | 2023-10-01 |
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| series | Brain Sciences |
| spelling | doaj.art-9e75151b72024e68971552094b28f6122023-11-19T15:53:37ZengMDPI AGBrain Sciences2076-34252023-10-011310148510.3390/brainsci13101485Serotonin Transporter mRNA Expression Is Reduced in the Peripheral Blood Mononuclear Cells of Subjects with Major Depression but Normal in FibromyalgiaGaël Villanueva-Charbonneau0Stéphane Potvin1Serge Marchand2Alexander McIntyre3Diane McIntosh4Alain Bissonnette5Alain Gendron6Charles-Édouard Giguère7Marie-Ève Koué8Édouard Kouassi9Département de Pharmacologie et Physiologie, Université de Montréal, Montréal, QC H1T 1C8, CanadaCentre de Recherche de l’Institut Universitaire en Santé Mentale de Montréal, Montréal, QC H1N 3V2, CanadaDepartment of Surgery, Faculty of Medicine, University of Sherbrooke, Sherbrooke, QC J1K 2R1, CanadaPenticton Regional Hospital, Penticton, BC V0H 1K0, CanadaDepartment of Psychiatry, University of British Columbia, Vancouver, BC V6T 1Z4, CanadaClinique du Campanile, Québec, QC G1X 4G6, CanadaAstraZeneca Pharmaceuticals, Mississauga, ON L4Y 1M4, CanadaCentre de Recherche de l’Institut Universitaire en Santé Mentale de Montréal, Montréal, QC H1N 3V2, CanadaDepartment of Biochemistry and Molecular Medicine, University of Montreal, Montreal, QC H3T 1J4, CanadaCentre de Recherche de l’Institut Universitaire en Santé Mentale de Montréal, Montréal, QC H1N 3V2, CanadaBackground: Fibromyalgia (FM) and major depression disorder (MDD) frequently co-occur. Both disorders may share common serotonergic alterations, although there is less evidence of such alterations in FM. It is also unclear as to whether these alterations are persistent over time or transient. The objectives of this study were to (i) examine the changes in mRNA expression of serotonin transporter (SERT) on the surface of peripheral blood mononuclear cells (PBMCs) in FM, MDD, and the FM + MDD subjects compared to healthy controls, and to (ii) evaluate the effect of drug treatment on SERT expression. Methods: PBMCs were isolated from FM, MDD, FM + MDD, and control subjects. SERT expression was analyzed at the mRNA level via quantitative real-time polymerase chain reaction. Statistical analyses were performed using analyses of variance and linear mixed-effects models. Results: SERT mRNA expression was significantly reduced in MDD subjects compared to controls (<i>p</i> < 0.001), but not in FM nor in FM + MDD subjects. Although the drug treatments improved symptoms in FM, MDD, and FM + MDD subjects, they had no significant effect on SERT mRNA expression. Conclusions: These results corroborate the role of the SERT in the pathophysiology of MDD, but not in FM, and show that the decreased mRNA expression of SERT is a persistent, rather than transient, phenomenon.https://www.mdpi.com/2076-3425/13/10/1485dopamine transporterserotonin transportermajor depressionfibromyalgiaquetiapine |
| spellingShingle | Gaël Villanueva-Charbonneau Stéphane Potvin Serge Marchand Alexander McIntyre Diane McIntosh Alain Bissonnette Alain Gendron Charles-Édouard Giguère Marie-Ève Koué Édouard Kouassi Serotonin Transporter mRNA Expression Is Reduced in the Peripheral Blood Mononuclear Cells of Subjects with Major Depression but Normal in Fibromyalgia Brain Sciences dopamine transporter serotonin transporter major depression fibromyalgia quetiapine |
| title | Serotonin Transporter mRNA Expression Is Reduced in the Peripheral Blood Mononuclear Cells of Subjects with Major Depression but Normal in Fibromyalgia |
| title_full | Serotonin Transporter mRNA Expression Is Reduced in the Peripheral Blood Mononuclear Cells of Subjects with Major Depression but Normal in Fibromyalgia |
| title_fullStr | Serotonin Transporter mRNA Expression Is Reduced in the Peripheral Blood Mononuclear Cells of Subjects with Major Depression but Normal in Fibromyalgia |
| title_full_unstemmed | Serotonin Transporter mRNA Expression Is Reduced in the Peripheral Blood Mononuclear Cells of Subjects with Major Depression but Normal in Fibromyalgia |
| title_short | Serotonin Transporter mRNA Expression Is Reduced in the Peripheral Blood Mononuclear Cells of Subjects with Major Depression but Normal in Fibromyalgia |
| title_sort | serotonin transporter mrna expression is reduced in the peripheral blood mononuclear cells of subjects with major depression but normal in fibromyalgia |
| topic | dopamine transporter serotonin transporter major depression fibromyalgia quetiapine |
| url | https://www.mdpi.com/2076-3425/13/10/1485 |
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