Composition of the sputum bacterial microbiome of patients with different pathomorphological forms of non-small-cell lung cancer
Recent studies have shown that the bacterial microbiome of the respiratory tract influences the development of lung cancer. Changes in the composition of the microbiome are observed in patients with chronic inflammatory processes. Such microbiome changes may include the occurrence of bacteria that c...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2024-04-01
|
| Series: | Вавиловский журнал генетики и селекции |
| Subjects: | |
| Online Access: | https://vavilov.elpub.ru/jour/article/view/4091 |
| _version_ | 1797213960511946752 |
|---|---|
| author | V. G. Druzhinin E. D. Baranova P. S. Demenkov L. V. Matskova A. V. Larionov |
| author_facet | V. G. Druzhinin E. D. Baranova P. S. Demenkov L. V. Matskova A. V. Larionov |
| author_sort | V. G. Druzhinin |
| collection | DOAJ |
| description | Recent studies have shown that the bacterial microbiome of the respiratory tract influences the development of lung cancer. Changes in the composition of the microbiome are observed in patients with chronic inflammatory processes. Such microbiome changes may include the occurrence of bacteria that cause oxidative stress and that are capable of causing genome damage in the cells of the host organism directly and indirectly. To date, the composition of the respiratory microbiome in patients with various histological variants of lung cancer has not been studied. In the present study, we determined the taxonomic composition of the sputum microbiome of 52 patients with squamous cell carcinoma of the lung, 52 patients with lung adenocarcinoma and 52 healthy control donors, using next-generation sequencing (NGS) on the V3-V4 region of the bacterial gene encoding 16S rRNA. The sputum microbiomes of patients with different histological types of lung cancer and controls did not show significant differences in terms of the species richness index (Shannon); however, the patients differed from the controls in terms of evenness index (Pielou). The structures of bacterial communities (beta diversity) in the adenocarcinoma and squamous cell carcinoma groups were also similar; however, when analyzed according to the matrix constructed by the Bray–Curtis method, there were differences between patients with squamous cell carcinoma and healthy subjects, but not between those with adenocarcinoma and controls. Using the LEFse method it was possible to identify an increase in the content of Bacillota (Streptococcus and Bacillus) and Actinomycetota (Rothia) in the sputum of patients with squamous cell carcinoma when compared with samples from patients with adenocarcinoma. There were no differences in the content of bacteria between the samples of patients with adenocarcinoma and the control ones. The content of representatives of the genera Streptococcus, Bacillus, Peptostreptococcus (phylum Bacillota), Prevotella, Macellibacteroides (phylum Bacteroidota), Rothia (phylum Actinomycetota) and Actinobacillus (phylum Pseudomonadota) was increased in the microbiome of sputum samples from patients with squamous cell carcinoma, compared with the control. Thus, the sputum bacterial microbiome of patients with different histological types of non-small-cell lung cancer has significant differences. Further research should be devoted to the search for microbiome biomarkers of lung cancer at the level of bacterial species using whole-genome sequencing. |
| first_indexed | 2024-04-24T11:06:35Z |
| format | Article |
| id | doaj.art-9e759890a23c4cc2824bc2752acc5866 |
| institution | Directory Open Access Journal |
| issn | 2500-3259 |
| language | English |
| last_indexed | 2024-04-24T11:06:35Z |
| publishDate | 2024-04-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj.art-9e759890a23c4cc2824bc2752acc58662024-04-11T15:31:07ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592024-04-0128220421410.18699/vjgb-24-251456Composition of the sputum bacterial microbiome of patients with different pathomorphological forms of non-small-cell lung cancerV. G. Druzhinin0E. D. Baranova1P. S. Demenkov2L. V. Matskova3A. V. Larionov4Kemerovo State University; Kemerovo State Medical UniversityKemerovo State UniversityInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesKarolinska InstituteKemerovo State UniversityRecent studies have shown that the bacterial microbiome of the respiratory tract influences the development of lung cancer. Changes in the composition of the microbiome are observed in patients with chronic inflammatory processes. Such microbiome changes may include the occurrence of bacteria that cause oxidative stress and that are capable of causing genome damage in the cells of the host organism directly and indirectly. To date, the composition of the respiratory microbiome in patients with various histological variants of lung cancer has not been studied. In the present study, we determined the taxonomic composition of the sputum microbiome of 52 patients with squamous cell carcinoma of the lung, 52 patients with lung adenocarcinoma and 52 healthy control donors, using next-generation sequencing (NGS) on the V3-V4 region of the bacterial gene encoding 16S rRNA. The sputum microbiomes of patients with different histological types of lung cancer and controls did not show significant differences in terms of the species richness index (Shannon); however, the patients differed from the controls in terms of evenness index (Pielou). The structures of bacterial communities (beta diversity) in the adenocarcinoma and squamous cell carcinoma groups were also similar; however, when analyzed according to the matrix constructed by the Bray–Curtis method, there were differences between patients with squamous cell carcinoma and healthy subjects, but not between those with adenocarcinoma and controls. Using the LEFse method it was possible to identify an increase in the content of Bacillota (Streptococcus and Bacillus) and Actinomycetota (Rothia) in the sputum of patients with squamous cell carcinoma when compared with samples from patients with adenocarcinoma. There were no differences in the content of bacteria between the samples of patients with adenocarcinoma and the control ones. The content of representatives of the genera Streptococcus, Bacillus, Peptostreptococcus (phylum Bacillota), Prevotella, Macellibacteroides (phylum Bacteroidota), Rothia (phylum Actinomycetota) and Actinobacillus (phylum Pseudomonadota) was increased in the microbiome of sputum samples from patients with squamous cell carcinoma, compared with the control. Thus, the sputum bacterial microbiome of patients with different histological types of non-small-cell lung cancer has significant differences. Further research should be devoted to the search for microbiome biomarkers of lung cancer at the level of bacterial species using whole-genome sequencing.https://vavilov.elpub.ru/jour/article/view/4091non-small cell lung cancersquamous cell lung cancerlung adenocarcinomabacterial microbiomesputumtaxonomic composition16s rrnangs sequencing |
| spellingShingle | V. G. Druzhinin E. D. Baranova P. S. Demenkov L. V. Matskova A. V. Larionov Composition of the sputum bacterial microbiome of patients with different pathomorphological forms of non-small-cell lung cancer Вавиловский журнал генетики и селекции non-small cell lung cancer squamous cell lung cancer lung adenocarcinoma bacterial microbiome sputum taxonomic composition 16s rrna ngs sequencing |
| title | Composition of the sputum bacterial microbiome of patients with different pathomorphological forms of non-small-cell lung cancer |
| title_full | Composition of the sputum bacterial microbiome of patients with different pathomorphological forms of non-small-cell lung cancer |
| title_fullStr | Composition of the sputum bacterial microbiome of patients with different pathomorphological forms of non-small-cell lung cancer |
| title_full_unstemmed | Composition of the sputum bacterial microbiome of patients with different pathomorphological forms of non-small-cell lung cancer |
| title_short | Composition of the sputum bacterial microbiome of patients with different pathomorphological forms of non-small-cell lung cancer |
| title_sort | composition of the sputum bacterial microbiome of patients with different pathomorphological forms of non small cell lung cancer |
| topic | non-small cell lung cancer squamous cell lung cancer lung adenocarcinoma bacterial microbiome sputum taxonomic composition 16s rrna ngs sequencing |
| url | https://vavilov.elpub.ru/jour/article/view/4091 |
| work_keys_str_mv | AT vgdruzhinin compositionofthesputumbacterialmicrobiomeofpatientswithdifferentpathomorphologicalformsofnonsmallcelllungcancer AT edbaranova compositionofthesputumbacterialmicrobiomeofpatientswithdifferentpathomorphologicalformsofnonsmallcelllungcancer AT psdemenkov compositionofthesputumbacterialmicrobiomeofpatientswithdifferentpathomorphologicalformsofnonsmallcelllungcancer AT lvmatskova compositionofthesputumbacterialmicrobiomeofpatientswithdifferentpathomorphologicalformsofnonsmallcelllungcancer AT avlarionov compositionofthesputumbacterialmicrobiomeofpatientswithdifferentpathomorphologicalformsofnonsmallcelllungcancer |