mRNA poly(A)-tail changes specified by deadenylation broadly reshape translation in Drosophila oocytes and early embryos
Because maturing oocytes and early embryos lack appreciable transcription, posttranscriptional regulatory processes control their development. To better understand this control, we profiled translational efficiencies and poly(A)-tail lengths throughout Drosophila oocyte maturation and early embryoni...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2016-07-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/16955 |
| _version_ | 1828168813612367872 |
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| author | Stephen W Eichhorn Alexander O Subtelny Iva Kronja Jamie C Kwasnieski Terry L Orr-Weaver David P Bartel |
| author_facet | Stephen W Eichhorn Alexander O Subtelny Iva Kronja Jamie C Kwasnieski Terry L Orr-Weaver David P Bartel |
| author_sort | Stephen W Eichhorn |
| collection | DOAJ |
| description | Because maturing oocytes and early embryos lack appreciable transcription, posttranscriptional regulatory processes control their development. To better understand this control, we profiled translational efficiencies and poly(A)-tail lengths throughout Drosophila oocyte maturation and early embryonic development. The correspondence between translational-efficiency changes and tail-length changes indicated that tail-length changes broadly regulate translation until gastrulation, when this coupling disappears. During egg activation, relative changes in poly(A)-tail length, and thus translational efficiency, were largely retained in the absence of cytoplasmic polyadenylation, which indicated that selective poly(A)-tail shortening primarily specifies these changes. Many translational changes depended on PAN GU and Smaug, and these changes were largely attributable to tail-length changes. Our results also revealed the presence of tail-length–independent mechanisms that maintained translation despite tail-length shortening during oocyte maturation, and prevented essentially all translation of bicoid and several other mRNAs before egg activation. In addition to these fundamental insights, our results provide valuable resources for future studies. |
| first_indexed | 2024-04-12T02:41:00Z |
| format | Article |
| id | doaj.art-9e766beef5c54d13b5626e53ed04c166 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:41:00Z |
| publishDate | 2016-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-9e766beef5c54d13b5626e53ed04c1662022-12-22T03:51:18ZengeLife Sciences Publications LtdeLife2050-084X2016-07-01510.7554/eLife.16955mRNA poly(A)-tail changes specified by deadenylation broadly reshape translation in Drosophila oocytes and early embryosStephen W Eichhorn0https://orcid.org/0000-0002-6410-4699Alexander O Subtelny1Iva Kronja2Jamie C Kwasnieski3Terry L Orr-Weaver4David P Bartel5https://orcid.org/0000-0002-3872-2856Howard Hughes Medical Institute, Whitehead Institute for Biomedical Research, Cambridge, United States; Department of Biology, Massachusetts Institute of Technology, Cambridge, United StatesHoward Hughes Medical Institute, Whitehead Institute for Biomedical Research, Cambridge, United States; Department of Biology, Massachusetts Institute of Technology, Cambridge, United States; Harvard-MIT Division of Health Sciences and Technology, Cambridge, United StatesDepartment of Biology, Massachusetts Institute of Technology, Cambridge, United States; Whitehead Institute for Biomedical Research, Cambridge, United StatesHoward Hughes Medical Institute, Whitehead Institute for Biomedical Research, Cambridge, United States; Department of Biology, Massachusetts Institute of Technology, Cambridge, United StatesDepartment of Biology, Massachusetts Institute of Technology, Cambridge, United States; Whitehead Institute for Biomedical Research, Cambridge, United StatesHoward Hughes Medical Institute, Whitehead Institute for Biomedical Research, Cambridge, United States; Department of Biology, Massachusetts Institute of Technology, Cambridge, United StatesBecause maturing oocytes and early embryos lack appreciable transcription, posttranscriptional regulatory processes control their development. To better understand this control, we profiled translational efficiencies and poly(A)-tail lengths throughout Drosophila oocyte maturation and early embryonic development. The correspondence between translational-efficiency changes and tail-length changes indicated that tail-length changes broadly regulate translation until gastrulation, when this coupling disappears. During egg activation, relative changes in poly(A)-tail length, and thus translational efficiency, were largely retained in the absence of cytoplasmic polyadenylation, which indicated that selective poly(A)-tail shortening primarily specifies these changes. Many translational changes depended on PAN GU and Smaug, and these changes were largely attributable to tail-length changes. Our results also revealed the presence of tail-length–independent mechanisms that maintained translation despite tail-length shortening during oocyte maturation, and prevented essentially all translation of bicoid and several other mRNAs before egg activation. In addition to these fundamental insights, our results provide valuable resources for future studies.https://elifesciences.org/articles/16955Drosophila oogenesisDrosophila embryogenesisposttranscriptional gene regulationribosome footprint profilingPAL-seqwispy |
| spellingShingle | Stephen W Eichhorn Alexander O Subtelny Iva Kronja Jamie C Kwasnieski Terry L Orr-Weaver David P Bartel mRNA poly(A)-tail changes specified by deadenylation broadly reshape translation in Drosophila oocytes and early embryos eLife Drosophila oogenesis Drosophila embryogenesis posttranscriptional gene regulation ribosome footprint profiling PAL-seq wispy |
| title | mRNA poly(A)-tail changes specified by deadenylation broadly reshape translation in Drosophila oocytes and early embryos |
| title_full | mRNA poly(A)-tail changes specified by deadenylation broadly reshape translation in Drosophila oocytes and early embryos |
| title_fullStr | mRNA poly(A)-tail changes specified by deadenylation broadly reshape translation in Drosophila oocytes and early embryos |
| title_full_unstemmed | mRNA poly(A)-tail changes specified by deadenylation broadly reshape translation in Drosophila oocytes and early embryos |
| title_short | mRNA poly(A)-tail changes specified by deadenylation broadly reshape translation in Drosophila oocytes and early embryos |
| title_sort | mrna poly a tail changes specified by deadenylation broadly reshape translation in drosophila oocytes and early embryos |
| topic | Drosophila oogenesis Drosophila embryogenesis posttranscriptional gene regulation ribosome footprint profiling PAL-seq wispy |
| url | https://elifesciences.org/articles/16955 |
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