Chrysophanol Mitigates T Cell Activation by Regulating the Expression of CD40 Ligand in Activated T Cells
Since T lymphocytes act as mediators between innate and acquired immunity, playing a crucial role in chronic inflammation, regulation of T cell activation to suitable levels is important. Chrysophanol, a member of the anthraquinone family, is known to possess several bioactivities, including anti-mi...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-08-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/21/17/6122 |
| _version_ | 1797555681236090880 |
|---|---|
| author | Hyun-Su Lee Gil-Saeng Jeong |
| author_facet | Hyun-Su Lee Gil-Saeng Jeong |
| author_sort | Hyun-Su Lee |
| collection | DOAJ |
| description | Since T lymphocytes act as mediators between innate and acquired immunity, playing a crucial role in chronic inflammation, regulation of T cell activation to suitable levels is important. Chrysophanol, a member of the anthraquinone family, is known to possess several bioactivities, including anti-microbial, anti-cancer, and hepatoprotective activities, however, little information is available on the inhibitory effects of chrysophanol on T cell activation. To elucidate whether chrysophanol regulates the activity of T cells, IL-2 expression in activated Jurkat T cells pretreated with chrysophanol was assessed. We showed that chrysophanol is not cytotoxic to Jurkat T cells under culture conditions using RPMI (Rosewell Park Memorial Institute) medium. Pretreatment with chrysophanol inhibited IL-2 production in T cells stimulated by CD3/28 antibodies or SEE-loaded Raji B cells. We also demonstrated that chrysophanol suppressed the expression of the CD40 ligand (CD40L) in activated T cells, and uncontrolled conjugation between B cells by pretreatment with chrysophanol reduced T cell activation. Besides, treatment with chrysophanol of Jurkat T cells blocked the NFκB signaling pathway, resulting in the abrogation of MAPK (mitogen-activated protein kinase) in activated T cells. These results provide novel insights into the suppressive effect of chrysophanol on T cell activation through the regulation of CD40L expression in T cell receptor-mediated stimulation conditions. |
| first_indexed | 2024-03-10T16:50:57Z |
| format | Article |
| id | doaj.art-9e795d2554cd4313939276796167bdd2 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T16:50:57Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9e795d2554cd4313939276796167bdd22023-11-20T11:18:36ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-08-012117612210.3390/ijms21176122Chrysophanol Mitigates T Cell Activation by Regulating the Expression of CD40 Ligand in Activated T CellsHyun-Su Lee0Gil-Saeng Jeong1College of Pharmacy, Keimyung University, Daegu 42601, KoreaCollege of Pharmacy, Keimyung University, Daegu 42601, KoreaSince T lymphocytes act as mediators between innate and acquired immunity, playing a crucial role in chronic inflammation, regulation of T cell activation to suitable levels is important. Chrysophanol, a member of the anthraquinone family, is known to possess several bioactivities, including anti-microbial, anti-cancer, and hepatoprotective activities, however, little information is available on the inhibitory effects of chrysophanol on T cell activation. To elucidate whether chrysophanol regulates the activity of T cells, IL-2 expression in activated Jurkat T cells pretreated with chrysophanol was assessed. We showed that chrysophanol is not cytotoxic to Jurkat T cells under culture conditions using RPMI (Rosewell Park Memorial Institute) medium. Pretreatment with chrysophanol inhibited IL-2 production in T cells stimulated by CD3/28 antibodies or SEE-loaded Raji B cells. We also demonstrated that chrysophanol suppressed the expression of the CD40 ligand (CD40L) in activated T cells, and uncontrolled conjugation between B cells by pretreatment with chrysophanol reduced T cell activation. Besides, treatment with chrysophanol of Jurkat T cells blocked the NFκB signaling pathway, resulting in the abrogation of MAPK (mitogen-activated protein kinase) in activated T cells. These results provide novel insights into the suppressive effect of chrysophanol on T cell activation through the regulation of CD40L expression in T cell receptor-mediated stimulation conditions.https://www.mdpi.com/1422-0067/21/17/6122T cellsIL-2CD40Limmunological synapseMAPK |
| spellingShingle | Hyun-Su Lee Gil-Saeng Jeong Chrysophanol Mitigates T Cell Activation by Regulating the Expression of CD40 Ligand in Activated T Cells International Journal of Molecular Sciences T cells IL-2 CD40L immunological synapse MAPK |
| title | Chrysophanol Mitigates T Cell Activation by Regulating the Expression of CD40 Ligand in Activated T Cells |
| title_full | Chrysophanol Mitigates T Cell Activation by Regulating the Expression of CD40 Ligand in Activated T Cells |
| title_fullStr | Chrysophanol Mitigates T Cell Activation by Regulating the Expression of CD40 Ligand in Activated T Cells |
| title_full_unstemmed | Chrysophanol Mitigates T Cell Activation by Regulating the Expression of CD40 Ligand in Activated T Cells |
| title_short | Chrysophanol Mitigates T Cell Activation by Regulating the Expression of CD40 Ligand in Activated T Cells |
| title_sort | chrysophanol mitigates t cell activation by regulating the expression of cd40 ligand in activated t cells |
| topic | T cells IL-2 CD40L immunological synapse MAPK |
| url | https://www.mdpi.com/1422-0067/21/17/6122 |
| work_keys_str_mv | AT hyunsulee chrysophanolmitigatestcellactivationbyregulatingtheexpressionofcd40ligandinactivatedtcells AT gilsaengjeong chrysophanolmitigatestcellactivationbyregulatingtheexpressionofcd40ligandinactivatedtcells |