Transcriptome-Based Analysis of the Response Mechanism of Leopard Coralgrouper Liver at Different Flow Velocities
The velocity of water can cause differences in the growth of inhabitant fish, thereby functioning as an important aquatic environmental factor. In order to explore the response mechanism of Leopard Coralgrouper (<i>Plectropomus leopardus</i>) under different flow velocities, <i>P....
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Fishes |
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| Online Access: | https://www.mdpi.com/2410-3888/7/5/279 |
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| author | Min Yang Jin Gao Hongji Ke Yongbo Wang Jinye Liu Xin Wen Shuyuan Fu Jiang Wang |
| author_facet | Min Yang Jin Gao Hongji Ke Yongbo Wang Jinye Liu Xin Wen Shuyuan Fu Jiang Wang |
| author_sort | Min Yang |
| collection | DOAJ |
| description | The velocity of water can cause differences in the growth of inhabitant fish, thereby functioning as an important aquatic environmental factor. In order to explore the response mechanism of Leopard Coralgrouper (<i>Plectropomus leopardus</i>) under different flow velocities, <i>P. leopardus</i> fish of the same sizes were fed in water characterized by three different flow rates (5 cm/s, 10 cm/s, and 20 cm/s) for 150 d, after which the differences in growth and the levels of liver oxidative stress kinase were analyzed. Additionally, potential underlying regulatory pathways were explored using comparative transcriptomic approaches. The results showed that in the feeding environment involving a flow rate of 10 cm/s, the weight increase of <i>P. leopardus</i> and its liver contents of glutathione peroxidase (GPX), alanine aminotransferase (ALT), and superoxide dismutase (T-SOD) were significantly higher than for the other two groups. After matching the comparative transcriptomic results between group pairs (5 cm/s and 10 cm/s, 5 cm/s and 20 cm/s, and 10 cm/s and 20 cm/s), a total of 225 common differential gene expressions were screened. The KEGG pathway enrichment analysis showed that these genes were mainly involved in protein processing in the endoplasmic reticulum, the TGF-β signaling pathway, fatty acid metabolism, fatty acid synthesis, and other pathways. These results not only provide a theoretical basis for the selection of flow velocity in the culture environment of <i>P. leopardus,</i> but also reveal its potential means of adaption to different flow velocities. |
| first_indexed | 2024-03-09T20:13:45Z |
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| id | doaj.art-9e7b34508a2a4931bc4d9c7afab991e8 |
| institution | Directory Open Access Journal |
| issn | 2410-3888 |
| language | English |
| last_indexed | 2024-03-09T20:13:45Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fishes |
| spelling | doaj.art-9e7b34508a2a4931bc4d9c7afab991e82023-11-24T00:06:42ZengMDPI AGFishes2410-38882022-10-017527910.3390/fishes7050279Transcriptome-Based Analysis of the Response Mechanism of Leopard Coralgrouper Liver at Different Flow VelocitiesMin Yang0Jin Gao1Hongji Ke2Yongbo Wang3Jinye Liu4Xin Wen5Shuyuan Fu6Jiang Wang7College of Science, Hainan University, Haikou 570228, ChinaHainan Provincial Key Laboratory of Tropical Maricultural Technologies, Hainan Provincial Engineering Research Center for Tropical Sea-Farming, Hainan Academy of Ocean and Fisheries Sciences, Haikou 571126, ChinaHainan Provincial Key Laboratory of Tropical Maricultural Technologies, Hainan Provincial Engineering Research Center for Tropical Sea-Farming, Hainan Academy of Ocean and Fisheries Sciences, Haikou 571126, ChinaHainan Provincial Key Laboratory of Tropical Maricultural Technologies, Hainan Provincial Engineering Research Center for Tropical Sea-Farming, Hainan Academy of Ocean and Fisheries Sciences, Haikou 571126, ChinaHainan Provincial Key Laboratory of Tropical Maricultural Technologies, Hainan Provincial Engineering Research Center for Tropical Sea-Farming, Hainan Academy of Ocean and Fisheries Sciences, Haikou 571126, ChinaCollege of Oceanography, Hainan University, Haikou 570228, ChinaHainan Provincial Key Laboratory of Tropical Maricultural Technologies, Hainan Provincial Engineering Research Center for Tropical Sea-Farming, Hainan Academy of Ocean and Fisheries Sciences, Haikou 571126, ChinaCollege of Science, Hainan University, Haikou 570228, ChinaThe velocity of water can cause differences in the growth of inhabitant fish, thereby functioning as an important aquatic environmental factor. In order to explore the response mechanism of Leopard Coralgrouper (<i>Plectropomus leopardus</i>) under different flow velocities, <i>P. leopardus</i> fish of the same sizes were fed in water characterized by three different flow rates (5 cm/s, 10 cm/s, and 20 cm/s) for 150 d, after which the differences in growth and the levels of liver oxidative stress kinase were analyzed. Additionally, potential underlying regulatory pathways were explored using comparative transcriptomic approaches. The results showed that in the feeding environment involving a flow rate of 10 cm/s, the weight increase of <i>P. leopardus</i> and its liver contents of glutathione peroxidase (GPX), alanine aminotransferase (ALT), and superoxide dismutase (T-SOD) were significantly higher than for the other two groups. After matching the comparative transcriptomic results between group pairs (5 cm/s and 10 cm/s, 5 cm/s and 20 cm/s, and 10 cm/s and 20 cm/s), a total of 225 common differential gene expressions were screened. The KEGG pathway enrichment analysis showed that these genes were mainly involved in protein processing in the endoplasmic reticulum, the TGF-β signaling pathway, fatty acid metabolism, fatty acid synthesis, and other pathways. These results not only provide a theoretical basis for the selection of flow velocity in the culture environment of <i>P. leopardus,</i> but also reveal its potential means of adaption to different flow velocities.https://www.mdpi.com/2410-3888/7/5/279flow velocitiesgrowth<i>Plectropomus leopardus</i>transcriptomics |
| spellingShingle | Min Yang Jin Gao Hongji Ke Yongbo Wang Jinye Liu Xin Wen Shuyuan Fu Jiang Wang Transcriptome-Based Analysis of the Response Mechanism of Leopard Coralgrouper Liver at Different Flow Velocities Fishes flow velocities growth <i>Plectropomus leopardus</i> transcriptomics |
| title | Transcriptome-Based Analysis of the Response Mechanism of Leopard Coralgrouper Liver at Different Flow Velocities |
| title_full | Transcriptome-Based Analysis of the Response Mechanism of Leopard Coralgrouper Liver at Different Flow Velocities |
| title_fullStr | Transcriptome-Based Analysis of the Response Mechanism of Leopard Coralgrouper Liver at Different Flow Velocities |
| title_full_unstemmed | Transcriptome-Based Analysis of the Response Mechanism of Leopard Coralgrouper Liver at Different Flow Velocities |
| title_short | Transcriptome-Based Analysis of the Response Mechanism of Leopard Coralgrouper Liver at Different Flow Velocities |
| title_sort | transcriptome based analysis of the response mechanism of leopard coralgrouper liver at different flow velocities |
| topic | flow velocities growth <i>Plectropomus leopardus</i> transcriptomics |
| url | https://www.mdpi.com/2410-3888/7/5/279 |
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