Characteristics of <i>PoVIN3</i>, a Key Gene of Vernalization Pathway, Affects Flowering Time

Characteristics of <i>PoVIN3</i>, a Key Gene of Vernalization Pathway, Affects Flowering Time

The tree peony (<i>Paeonia</i> section <i>Moutan</i> DC.) is the candidate flower in China, with abundant germplasm resources and high ornamental value. However, the short and concentrated flowering period severely restricted the improvement of the economic value of tree peon...

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Bibliographic Details
Main Authors: Yuying Li, Can Wang, Qi Guo, Chengwei Song, Xiaohui Wang, Lili Guo, Xiaogai Hou
Format: Article
Language:English
Published: MDPI AG 2022-11-01
Series:International Journal of Molecular Sciences
Subjects:
tree peony
<i>PoVIN3</i>
flowering regulation
expression analysis
functional analysis
Online Access:https://www.mdpi.com/1422-0067/23/22/14003
Description
Summary:The tree peony (<i>Paeonia</i> section <i>Moutan</i> DC.) is the candidate flower in China, with abundant germplasm resources and high ornamental value. However, the short and concentrated flowering period severely restricted the improvement of the economic value of tree peonies. Based on the full-length transcriptome database of tree peonies, the <i>PoVIN3</i> (GenBank ID: OP341879), involved in the flowering regulation of tree peonies were identified and cloned for the first time. The <i>PoVIN3</i> was also characterized by bioinformatics methods, quantitative real-time PCR (qRT-PCR), and the establishment of a transgenic system. The expression levels of <i>PoVIN3</i> in seven different petals developmental stages were the highest at the initial flowering stage of the variant cultivar of <i>Paeonia ostii</i> ‘Fengdan,’ the initial decay stage of the normal flowering <i>Paeonia ostii</i> ‘Fengdan,’ and the half opening stage of the late flowering <i>Paeonia suffruticosa</i> ‘Lianhe.’ Tissue-specific expression analysis showed that the relative expression levels of <i>PoVIN3</i> were the highest in sepals of both normal flowering <i>Paeonia ostii</i> ‘Fengdan’ and the late flowering <i>Paeonia suffruticosa</i> ‘Lianhe,’ and the highest expression was in stamens of early flowering mutant <i>Paeonia ostii</i> ‘Fengdan.’ In addition, the flowering time of pCAMBIA2300-<i>PoVIN3</i> transgenic plants was significantly earlier than that of the wild-type, indicating that PoVIN3 could promote plant flowering. The results provide a theoretical basis for exploring the role of <i>PoVIN3</i> in the regulation of flowering in tree peonies.
ISSN:1661-6596
1422-0067

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