Characteristics of <i>PoVIN3</i>, a Key Gene of Vernalization Pathway, Affects Flowering Time
The tree peony (<i>Paeonia</i> section <i>Moutan</i> DC.) is the candidate flower in China, with abundant germplasm resources and high ornamental value. However, the short and concentrated flowering period severely restricted the improvement of the economic value of tree peon...
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/22/14003 |
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| author | Yuying Li Can Wang Qi Guo Chengwei Song Xiaohui Wang Lili Guo Xiaogai Hou |
| author_facet | Yuying Li Can Wang Qi Guo Chengwei Song Xiaohui Wang Lili Guo Xiaogai Hou |
| author_sort | Yuying Li |
| collection | DOAJ |
| description | The tree peony (<i>Paeonia</i> section <i>Moutan</i> DC.) is the candidate flower in China, with abundant germplasm resources and high ornamental value. However, the short and concentrated flowering period severely restricted the improvement of the economic value of tree peonies. Based on the full-length transcriptome database of tree peonies, the <i>PoVIN3</i> (GenBank ID: OP341879), involved in the flowering regulation of tree peonies were identified and cloned for the first time. The <i>PoVIN3</i> was also characterized by bioinformatics methods, quantitative real-time PCR (qRT-PCR), and the establishment of a transgenic system. The expression levels of <i>PoVIN3</i> in seven different petals developmental stages were the highest at the initial flowering stage of the variant cultivar of <i>Paeonia ostii</i> ‘Fengdan,’ the initial decay stage of the normal flowering <i>Paeonia ostii</i> ‘Fengdan,’ and the half opening stage of the late flowering <i>Paeonia suffruticosa</i> ‘Lianhe.’ Tissue-specific expression analysis showed that the relative expression levels of <i>PoVIN3</i> were the highest in sepals of both normal flowering <i>Paeonia ostii</i> ‘Fengdan’ and the late flowering <i>Paeonia suffruticosa</i> ‘Lianhe,’ and the highest expression was in stamens of early flowering mutant <i>Paeonia ostii</i> ‘Fengdan.’ In addition, the flowering time of pCAMBIA2300-<i>PoVIN3</i> transgenic plants was significantly earlier than that of the wild-type, indicating that PoVIN3 could promote plant flowering. The results provide a theoretical basis for exploring the role of <i>PoVIN3</i> in the regulation of flowering in tree peonies. |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9e7bb0a390a94df5a8bff2b14c07ed802023-11-24T08:36:59ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123221400310.3390/ijms232214003Characteristics of <i>PoVIN3</i>, a Key Gene of Vernalization Pathway, Affects Flowering TimeYuying Li0Can Wang1Qi Guo2Chengwei Song3Xiaohui Wang4Lili Guo5Xiaogai Hou6College of Agronomy/Tree Peony, Henan University of Science and Technology, Luoyang 471023, ChinaCollege of Agronomy/Tree Peony, Henan University of Science and Technology, Luoyang 471023, ChinaCollege of Agronomy/Tree Peony, Henan University of Science and Technology, Luoyang 471023, ChinaCollege of Agronomy/Tree Peony, Henan University of Science and Technology, Luoyang 471023, ChinaLuoyang Academy of Agriculture and Forestry Sciences, Luoyang 471023, ChinaCollege of Agronomy/Tree Peony, Henan University of Science and Technology, Luoyang 471023, ChinaCollege of Agronomy/Tree Peony, Henan University of Science and Technology, Luoyang 471023, ChinaThe tree peony (<i>Paeonia</i> section <i>Moutan</i> DC.) is the candidate flower in China, with abundant germplasm resources and high ornamental value. However, the short and concentrated flowering period severely restricted the improvement of the economic value of tree peonies. Based on the full-length transcriptome database of tree peonies, the <i>PoVIN3</i> (GenBank ID: OP341879), involved in the flowering regulation of tree peonies were identified and cloned for the first time. The <i>PoVIN3</i> was also characterized by bioinformatics methods, quantitative real-time PCR (qRT-PCR), and the establishment of a transgenic system. The expression levels of <i>PoVIN3</i> in seven different petals developmental stages were the highest at the initial flowering stage of the variant cultivar of <i>Paeonia ostii</i> ‘Fengdan,’ the initial decay stage of the normal flowering <i>Paeonia ostii</i> ‘Fengdan,’ and the half opening stage of the late flowering <i>Paeonia suffruticosa</i> ‘Lianhe.’ Tissue-specific expression analysis showed that the relative expression levels of <i>PoVIN3</i> were the highest in sepals of both normal flowering <i>Paeonia ostii</i> ‘Fengdan’ and the late flowering <i>Paeonia suffruticosa</i> ‘Lianhe,’ and the highest expression was in stamens of early flowering mutant <i>Paeonia ostii</i> ‘Fengdan.’ In addition, the flowering time of pCAMBIA2300-<i>PoVIN3</i> transgenic plants was significantly earlier than that of the wild-type, indicating that PoVIN3 could promote plant flowering. The results provide a theoretical basis for exploring the role of <i>PoVIN3</i> in the regulation of flowering in tree peonies.https://www.mdpi.com/1422-0067/23/22/14003tree peony<i>PoVIN3</i>flowering regulationexpression analysisfunctional analysis |
| spellingShingle | Yuying Li Can Wang Qi Guo Chengwei Song Xiaohui Wang Lili Guo Xiaogai Hou Characteristics of <i>PoVIN3</i>, a Key Gene of Vernalization Pathway, Affects Flowering Time International Journal of Molecular Sciences tree peony <i>PoVIN3</i> flowering regulation expression analysis functional analysis |
| title | Characteristics of <i>PoVIN3</i>, a Key Gene of Vernalization Pathway, Affects Flowering Time |
| title_full | Characteristics of <i>PoVIN3</i>, a Key Gene of Vernalization Pathway, Affects Flowering Time |
| title_fullStr | Characteristics of <i>PoVIN3</i>, a Key Gene of Vernalization Pathway, Affects Flowering Time |
| title_full_unstemmed | Characteristics of <i>PoVIN3</i>, a Key Gene of Vernalization Pathway, Affects Flowering Time |
| title_short | Characteristics of <i>PoVIN3</i>, a Key Gene of Vernalization Pathway, Affects Flowering Time |
| title_sort | characteristics of i povin3 i a key gene of vernalization pathway affects flowering time |
| topic | tree peony <i>PoVIN3</i> flowering regulation expression analysis functional analysis |
| url | https://www.mdpi.com/1422-0067/23/22/14003 |
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