Expression of COX-1, COX-2, 5-LOX and $$\hbox {CysLT}_2$$ CysLT2 in nasal polyps and bronchial tissue of patients with aspirin exacerbated airway disease
Abstract Background Aspirin exacerbated respiratory disease (AERD) is a disease of the upper and lower airways. It is characterized by severe asthma, chronic sinusitis with nasal polyps (CRSwNP) and intolerance towards nonsteroidal analgesics (NSAR). Arachidonic acid (AA) metabolites play an importa...
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| Format: | Article |
| Language: | English |
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BMC
2019-12-01
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| Series: | Allergy, Asthma & Clinical Immunology |
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| Online Access: | https://doi.org/10.1186/s13223-019-0395-5 |
| _version_ | 1818611494542114816 |
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| author | Monique Vorsprach Christoph Arens Stephan Knipping Dörte Jechorek Sabine Stegemann-Koniszewski Eva Lücke Jens Schreiber |
| author_facet | Monique Vorsprach Christoph Arens Stephan Knipping Dörte Jechorek Sabine Stegemann-Koniszewski Eva Lücke Jens Schreiber |
| author_sort | Monique Vorsprach |
| collection | DOAJ |
| description | Abstract Background Aspirin exacerbated respiratory disease (AERD) is a disease of the upper and lower airways. It is characterized by severe asthma, chronic sinusitis with nasal polyps (CRSwNP) and intolerance towards nonsteroidal analgesics (NSAR). Arachidonic acid (AA) metabolites play an important role in the pathogenesis of AERD. It is still unknown, whether metabolism of AA is comparable between the upper and lower airways as well as between patients with and without NSAR intolerance. Objective We sought to analyze differences in the expression of cyclooxygenases type 1 and 2 (COX-1, COX-2), arachidonate 5-lipoxygenase (5-LOX) and cysteinyl leukotriene receptor type 2 ($$\hbox {CysLT}_2$$ CysLT2 ) in nasal polyps and the bronchial mucosa of patients with aspirin intolerant asthma (AIA, $$n=23$$ n=23 ) as compared to patients with aspirin tolerant asthma (ATA, $$n=17$$ n=17 ) and a control group with nasal polyps, but without asthma (NPwA, $$n=15$$ n=15 ). Methods Tissue biopsies from nasal polyps and bronchial mucosa were obtained during surgical treatment of nasal polyps by endonasal functional endoscopic sinus surgery (FESS) under general anesthesia from intubated patients. Immunohistochemistry was used to analyze the expression of COX-1, COX-2, 5-LOX and $$\hbox {CysLT}_2$$ CysLT2 in nasal and bronchial mucosa. Categorization into the different patient groups was performed according to the patient history, clinical and laboratory data, pulmonary function and provocation tests, as well as allergy testing. Results We observed a stronger expression of 5-LOX and $$\hbox {CysLT}_2$$ CysLT2 in submucosal glands of nasal and bronchial tissue compared to epithelial expression. The expression of COX-1 and COX-2 was stronger in epithelia compared to submucosal glands. There was a similar expression of the enzymes and $$\hbox {CysLT}_2$$ CysLT2 between upper and lower airways in all patient groups. We did not detect any significant differences between the patient groups. Conclusions The AA-metabolizing enzymes and the $$\hbox {CysLT}_2$$ CysLT2 were expressed in a very similar way in different microscopic structures in samples of the upper and lower airways of individual patients. We did not detect differences between the patient groups indicating the pathogenetic role of AA metabolism in these disorders is independent of the presence of NSAR-intolerance. |
| first_indexed | 2024-12-16T15:31:13Z |
| format | Article |
| id | doaj.art-9e7bed16904f4bf9931d1163b9ac3223 |
| institution | Directory Open Access Journal |
| issn | 1710-1492 |
| language | English |
| last_indexed | 2024-12-16T15:31:13Z |
| publishDate | 2019-12-01 |
| publisher | BMC |
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| series | Allergy, Asthma & Clinical Immunology |
| spelling | doaj.art-9e7bed16904f4bf9931d1163b9ac32232022-12-21T22:26:21ZengBMCAllergy, Asthma & Clinical Immunology1710-14922019-12-0115111410.1186/s13223-019-0395-5Expression of COX-1, COX-2, 5-LOX and $$\hbox {CysLT}_2$$ CysLT2 in nasal polyps and bronchial tissue of patients with aspirin exacerbated airway diseaseMonique Vorsprach0Christoph Arens1Stephan Knipping2Dörte Jechorek3Sabine Stegemann-Koniszewski4Eva Lücke5Jens Schreiber6Departmemt of Pneumology, University Hospital Magdeburg, Medical Faculty, Otto-von-Guericke-UniversityDepartment of Otorhinolaryngology, Head- and Neck Surgery, University Hospital Magdeburg, Medical Faculty, Otto-von-Guericke-UniversityDepartment of Otorhinolaryngology, Head- and Neck Surgery, Plastical Surgery, Dessau Medical Center, Martin-Luther-University HalleDepartment of Pathology, University Hospital Magdeburg, Medical Faculty, Otto-von-Guericke-UniversityDepartmemt of Pneumology, University Hospital Magdeburg, Medical Faculty, Otto-von-Guericke-UniversityDepartmemt of Pneumology, University Hospital Magdeburg, Medical Faculty, Otto-von-Guericke-UniversityDepartmemt of Pneumology, University Hospital Magdeburg, Medical Faculty, Otto-von-Guericke-UniversityAbstract Background Aspirin exacerbated respiratory disease (AERD) is a disease of the upper and lower airways. It is characterized by severe asthma, chronic sinusitis with nasal polyps (CRSwNP) and intolerance towards nonsteroidal analgesics (NSAR). Arachidonic acid (AA) metabolites play an important role in the pathogenesis of AERD. It is still unknown, whether metabolism of AA is comparable between the upper and lower airways as well as between patients with and without NSAR intolerance. Objective We sought to analyze differences in the expression of cyclooxygenases type 1 and 2 (COX-1, COX-2), arachidonate 5-lipoxygenase (5-LOX) and cysteinyl leukotriene receptor type 2 ($$\hbox {CysLT}_2$$ CysLT2 ) in nasal polyps and the bronchial mucosa of patients with aspirin intolerant asthma (AIA, $$n=23$$ n=23 ) as compared to patients with aspirin tolerant asthma (ATA, $$n=17$$ n=17 ) and a control group with nasal polyps, but without asthma (NPwA, $$n=15$$ n=15 ). Methods Tissue biopsies from nasal polyps and bronchial mucosa were obtained during surgical treatment of nasal polyps by endonasal functional endoscopic sinus surgery (FESS) under general anesthesia from intubated patients. Immunohistochemistry was used to analyze the expression of COX-1, COX-2, 5-LOX and $$\hbox {CysLT}_2$$ CysLT2 in nasal and bronchial mucosa. Categorization into the different patient groups was performed according to the patient history, clinical and laboratory data, pulmonary function and provocation tests, as well as allergy testing. Results We observed a stronger expression of 5-LOX and $$\hbox {CysLT}_2$$ CysLT2 in submucosal glands of nasal and bronchial tissue compared to epithelial expression. The expression of COX-1 and COX-2 was stronger in epithelia compared to submucosal glands. There was a similar expression of the enzymes and $$\hbox {CysLT}_2$$ CysLT2 between upper and lower airways in all patient groups. We did not detect any significant differences between the patient groups. Conclusions The AA-metabolizing enzymes and the $$\hbox {CysLT}_2$$ CysLT2 were expressed in a very similar way in different microscopic structures in samples of the upper and lower airways of individual patients. We did not detect differences between the patient groups indicating the pathogenetic role of AA metabolism in these disorders is independent of the presence of NSAR-intolerance.https://doi.org/10.1186/s13223-019-0395-5AsthmaNasal polypsAspirin exacerbated respiratory diseaseArachidonic acidCyclooxygenasesLipoxygenases |
| spellingShingle | Monique Vorsprach Christoph Arens Stephan Knipping Dörte Jechorek Sabine Stegemann-Koniszewski Eva Lücke Jens Schreiber Expression of COX-1, COX-2, 5-LOX and $$\hbox {CysLT}_2$$ CysLT2 in nasal polyps and bronchial tissue of patients with aspirin exacerbated airway disease Allergy, Asthma & Clinical Immunology Asthma Nasal polyps Aspirin exacerbated respiratory disease Arachidonic acid Cyclooxygenases Lipoxygenases |
| title | Expression of COX-1, COX-2, 5-LOX and $$\hbox {CysLT}_2$$ CysLT2 in nasal polyps and bronchial tissue of patients with aspirin exacerbated airway disease |
| title_full | Expression of COX-1, COX-2, 5-LOX and $$\hbox {CysLT}_2$$ CysLT2 in nasal polyps and bronchial tissue of patients with aspirin exacerbated airway disease |
| title_fullStr | Expression of COX-1, COX-2, 5-LOX and $$\hbox {CysLT}_2$$ CysLT2 in nasal polyps and bronchial tissue of patients with aspirin exacerbated airway disease |
| title_full_unstemmed | Expression of COX-1, COX-2, 5-LOX and $$\hbox {CysLT}_2$$ CysLT2 in nasal polyps and bronchial tissue of patients with aspirin exacerbated airway disease |
| title_short | Expression of COX-1, COX-2, 5-LOX and $$\hbox {CysLT}_2$$ CysLT2 in nasal polyps and bronchial tissue of patients with aspirin exacerbated airway disease |
| title_sort | expression of cox 1 cox 2 5 lox and hbox cyslt 2 cyslt2 in nasal polyps and bronchial tissue of patients with aspirin exacerbated airway disease |
| topic | Asthma Nasal polyps Aspirin exacerbated respiratory disease Arachidonic acid Cyclooxygenases Lipoxygenases |
| url | https://doi.org/10.1186/s13223-019-0395-5 |
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