Inhibitors of the RBD-ACE-2 Found among a Wide Range of Dyes by the Immunoassay Method
Angiotensin-converting enzyme 2 (ACE2), the functional receptor of SARS-CoV-2, plays a crucial role in the pathogenesis of COVID-19. ACE2 targeting holds the promise for preventing and inhibiting SARS-CoV-2 infection. In this work, we describe the development and use of a test system based on compet...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/11/2/135 |
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| author | Ekaterina D. Mordvinova Polina A. Nikitina Olga I. Yarovaya Ekaterina A. Volosnikova Denis E. Murashkin Anastasiya A. Isaeva Tatiana Y. Koldaeva Valery P. Perevalov Nariman F. Salakhutdinov Dmitriy N. Shcherbakov |
| author_facet | Ekaterina D. Mordvinova Polina A. Nikitina Olga I. Yarovaya Ekaterina A. Volosnikova Denis E. Murashkin Anastasiya A. Isaeva Tatiana Y. Koldaeva Valery P. Perevalov Nariman F. Salakhutdinov Dmitriy N. Shcherbakov |
| author_sort | Ekaterina D. Mordvinova |
| collection | DOAJ |
| description | Angiotensin-converting enzyme 2 (ACE2), the functional receptor of SARS-CoV-2, plays a crucial role in the pathogenesis of COVID-19. ACE2 targeting holds the promise for preventing and inhibiting SARS-CoV-2 infection. In this work, we describe the development and use of a test system based on competitive ELISA for the primary screening of potential antiviral compounds. We studied the activity of the library of dyes of different groups. Several dyes (ortho-cresolphthalein, eosin (free acid), eosin (Na salt)) that inhibited the interaction of ACE2 with the spike proteins of SARS-CoV-2 have been identified among the candidates. A potential antiviral drug, methylene blue, did not show activity in our study. We believe that our results can help in the further search for inhibitors of interaction between the coronavirus spike protein and ACE2 receptor. |
| first_indexed | 2024-03-11T09:00:08Z |
| format | Article |
| id | doaj.art-9e7c8c4cc3f945dfb9c9c74a84b1a036 |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-11T09:00:08Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-9e7c8c4cc3f945dfb9c9c74a84b1a0362023-11-16T19:46:46ZengMDPI AGChemosensors2227-90402023-02-0111213510.3390/chemosensors11020135Inhibitors of the RBD-ACE-2 Found among a Wide Range of Dyes by the Immunoassay MethodEkaterina D. Mordvinova0Polina A. Nikitina1Olga I. Yarovaya2Ekaterina A. Volosnikova3Denis E. Murashkin4Anastasiya A. Isaeva5Tatiana Y. Koldaeva6Valery P. Perevalov7Nariman F. Salakhutdinov8Dmitriy N. Shcherbakov9Department of Medicinal Chemistry, N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry SB RAS, Lavrentyev ave., 9, 630090 Novosibirsk, RussiaDepartment of Fine Organic Synthesis and Chemistry of Dyes, D.I. Mendeleev University of Chemical Technology of Russia, Miusskaya sq., 9, 125047 Moscow, RussiaDepartment of Medicinal Chemistry, N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry SB RAS, Lavrentyev ave., 9, 630090 Novosibirsk, RussiaState Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, RussiaState Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, RussiaState Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, RussiaDepartment of Fine Organic Synthesis and Chemistry of Dyes, D.I. Mendeleev University of Chemical Technology of Russia, Miusskaya sq., 9, 125047 Moscow, RussiaDepartment of Fine Organic Synthesis and Chemistry of Dyes, D.I. Mendeleev University of Chemical Technology of Russia, Miusskaya sq., 9, 125047 Moscow, RussiaDepartment of Medicinal Chemistry, N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry SB RAS, Lavrentyev ave., 9, 630090 Novosibirsk, RussiaState Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, RussiaAngiotensin-converting enzyme 2 (ACE2), the functional receptor of SARS-CoV-2, plays a crucial role in the pathogenesis of COVID-19. ACE2 targeting holds the promise for preventing and inhibiting SARS-CoV-2 infection. In this work, we describe the development and use of a test system based on competitive ELISA for the primary screening of potential antiviral compounds. We studied the activity of the library of dyes of different groups. Several dyes (ortho-cresolphthalein, eosin (free acid), eosin (Na salt)) that inhibited the interaction of ACE2 with the spike proteins of SARS-CoV-2 have been identified among the candidates. A potential antiviral drug, methylene blue, did not show activity in our study. We believe that our results can help in the further search for inhibitors of interaction between the coronavirus spike protein and ACE2 receptor.https://www.mdpi.com/2227-9040/11/2/135SARS-CoV-2 virusspike SELISARBDdyes |
| spellingShingle | Ekaterina D. Mordvinova Polina A. Nikitina Olga I. Yarovaya Ekaterina A. Volosnikova Denis E. Murashkin Anastasiya A. Isaeva Tatiana Y. Koldaeva Valery P. Perevalov Nariman F. Salakhutdinov Dmitriy N. Shcherbakov Inhibitors of the RBD-ACE-2 Found among a Wide Range of Dyes by the Immunoassay Method Chemosensors SARS-CoV-2 virus spike S ELISA RBD dyes |
| title | Inhibitors of the RBD-ACE-2 Found among a Wide Range of Dyes by the Immunoassay Method |
| title_full | Inhibitors of the RBD-ACE-2 Found among a Wide Range of Dyes by the Immunoassay Method |
| title_fullStr | Inhibitors of the RBD-ACE-2 Found among a Wide Range of Dyes by the Immunoassay Method |
| title_full_unstemmed | Inhibitors of the RBD-ACE-2 Found among a Wide Range of Dyes by the Immunoassay Method |
| title_short | Inhibitors of the RBD-ACE-2 Found among a Wide Range of Dyes by the Immunoassay Method |
| title_sort | inhibitors of the rbd ace 2 found among a wide range of dyes by the immunoassay method |
| topic | SARS-CoV-2 virus spike S ELISA RBD dyes |
| url | https://www.mdpi.com/2227-9040/11/2/135 |
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