Cyclophilin B serum levels present variations across the menstrual cycle
Abstract Cyclophilins are a family of chaperones involved in inflammation and cell death. Cyclophilin B is released by inflammatory cells and acts through the receptor CD147, affecting matrix metalloproteases release, whilst cyclophilin D participates in hypoxia-induced apoptosis. Previous studies r...
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| Format: | Article |
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Nature Portfolio
2023-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-37322-7 |
| _version_ | 1797795729216897024 |
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| author | Rebeca Alvariño Cristina Gil-Mouce Manuel A. Botana Sandra Gegunde Jesús González-Jartín Mercedes R. Vieytes Amparo Alfonso Luis M. Botana |
| author_facet | Rebeca Alvariño Cristina Gil-Mouce Manuel A. Botana Sandra Gegunde Jesús González-Jartín Mercedes R. Vieytes Amparo Alfonso Luis M. Botana |
| author_sort | Rebeca Alvariño |
| collection | DOAJ |
| description | Abstract Cyclophilins are a family of chaperones involved in inflammation and cell death. Cyclophilin B is released by inflammatory cells and acts through the receptor CD147, affecting matrix metalloproteases release, whilst cyclophilin D participates in hypoxia-induced apoptosis. Previous studies related hormones like estradiol or prolactin to these proteins, however, their blood concentrations across the menstrual cycle have not been determined. In this work, eleven healthy women (BMI: 21.8 kg/m2) were monitored during a single menstrual cycle, making blood extractions at follicular, periovulatory and mid-luteal phases. Hormone and cyclophilin levels were determined in each phase. Statistical differences were determined by repeated measures ANOVA and estimated marginal means tests, or by Friedman and Dunn-Bonferroni tests for parametric and non-parametric variables, respectively. Bivariate correlations were evaluated with the Spearman coefficient. Cyclophilin B concentrations presented significant differences during the menstrual cycle (p = 0.012). The highest levels of this protein were found at follicular extraction, followed by a decrease at periovulatory phase and a slight increase at mid-luteal phase. Cyclophilin D showed the same profile, although statistical significance was not reached. This immunophilin exhibited a positive correlation with luteinizing hormone at periovulatory phase (r = 0.743, p = 0.009) and with follicle stimulating hormone at mid-luteal phase (r = 0.633, p = 0.036). This is the first study describing the changes in cyclophilin B concentrations across the menstrual cycle, as well as the association of luteinizing and follicle stimulating hormones with cyclophilin D. These results suggest a role of these proteins in the cyclic inflammatory events that affect female reproductive system that should be explored. |
| first_indexed | 2024-03-13T03:22:26Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T03:22:26Z |
| publishDate | 2023-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9e7d78d6ab8b46409c514cbe047407222023-06-25T11:14:19ZengNature PortfolioScientific Reports2045-23222023-06-011311710.1038/s41598-023-37322-7Cyclophilin B serum levels present variations across the menstrual cycleRebeca Alvariño0Cristina Gil-Mouce1Manuel A. Botana2Sandra Gegunde3Jesús González-Jartín4Mercedes R. Vieytes5Amparo Alfonso6Luis M. Botana7Departamento de Farmacología, Facultad de Veterinaria, Universidad de Santiago de CompostelaDepartamento de Endocrinología y Nutrición, Hospital Universitario Lucus AugustiDepartamento de Endocrinología y Nutrición, Hospital Universitario Lucus AugustiDepartamento de Farmacología, Facultad de Veterinaria, Universidad de Santiago de CompostelaDepartamento de Farmacología, Facultad de Veterinaria, Universidad de Santiago de CompostelaGrupo Investigación Biodiscovery, IDISDepartamento de Farmacología, Facultad de Veterinaria, Universidad de Santiago de CompostelaDepartamento de Farmacología, Facultad de Veterinaria, Universidad de Santiago de CompostelaAbstract Cyclophilins are a family of chaperones involved in inflammation and cell death. Cyclophilin B is released by inflammatory cells and acts through the receptor CD147, affecting matrix metalloproteases release, whilst cyclophilin D participates in hypoxia-induced apoptosis. Previous studies related hormones like estradiol or prolactin to these proteins, however, their blood concentrations across the menstrual cycle have not been determined. In this work, eleven healthy women (BMI: 21.8 kg/m2) were monitored during a single menstrual cycle, making blood extractions at follicular, periovulatory and mid-luteal phases. Hormone and cyclophilin levels were determined in each phase. Statistical differences were determined by repeated measures ANOVA and estimated marginal means tests, or by Friedman and Dunn-Bonferroni tests for parametric and non-parametric variables, respectively. Bivariate correlations were evaluated with the Spearman coefficient. Cyclophilin B concentrations presented significant differences during the menstrual cycle (p = 0.012). The highest levels of this protein were found at follicular extraction, followed by a decrease at periovulatory phase and a slight increase at mid-luteal phase. Cyclophilin D showed the same profile, although statistical significance was not reached. This immunophilin exhibited a positive correlation with luteinizing hormone at periovulatory phase (r = 0.743, p = 0.009) and with follicle stimulating hormone at mid-luteal phase (r = 0.633, p = 0.036). This is the first study describing the changes in cyclophilin B concentrations across the menstrual cycle, as well as the association of luteinizing and follicle stimulating hormones with cyclophilin D. These results suggest a role of these proteins in the cyclic inflammatory events that affect female reproductive system that should be explored.https://doi.org/10.1038/s41598-023-37322-7 |
| spellingShingle | Rebeca Alvariño Cristina Gil-Mouce Manuel A. Botana Sandra Gegunde Jesús González-Jartín Mercedes R. Vieytes Amparo Alfonso Luis M. Botana Cyclophilin B serum levels present variations across the menstrual cycle Scientific Reports |
| title | Cyclophilin B serum levels present variations across the menstrual cycle |
| title_full | Cyclophilin B serum levels present variations across the menstrual cycle |
| title_fullStr | Cyclophilin B serum levels present variations across the menstrual cycle |
| title_full_unstemmed | Cyclophilin B serum levels present variations across the menstrual cycle |
| title_short | Cyclophilin B serum levels present variations across the menstrual cycle |
| title_sort | cyclophilin b serum levels present variations across the menstrual cycle |
| url | https://doi.org/10.1038/s41598-023-37322-7 |
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