Environmental Signals Modulate the Expression of an Indole-3-Acetic Acid Biosynthetic Gene in Erwinia herbicola
The ipdC gene of Erwinia herbicola strain 299R encodes an indolepyruvate decarboxylase involved in the biosynthesis of indole-3-acetic acid (IAA). Transcriptional fusions of ipdC to an ice nucleation reporter gene (inaZ) were used to study the expression of ipdC in vitro and in situ on plants. ipdC...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
1997-05-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1997.10.4.499 |
| _version_ | 1811290215824752640 |
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| author | Maria T. Brandl Steven E. Lindow |
| author_facet | Maria T. Brandl Steven E. Lindow |
| author_sort | Maria T. Brandl |
| collection | DOAJ |
| description | The ipdC gene of Erwinia herbicola strain 299R encodes an indolepyruvate decarboxylase involved in the biosynthesis of indole-3-acetic acid (IAA). Transcriptional fusions of ipdC to an ice nucleation reporter gene (inaZ) were used to study the expression of ipdC in vitro and in situ on plants. ipdC was expressed only at low levels in liquid media and independently of factors such as richness of the medium, pH, nitrogen availability, the presence of l-tryptophan or oxygen, and growth phase of the culture. However, the transcriptional activity of ipdC increased approximately 18-fold under low solute and matric potentials in culture. ipdC was also induced 32-fold on leaves of bean and tobacco and 1,000-fold on pear flowers. This is the first report of the plant-inducible transcription of a bacterial IAA biosynthetic gene. It strongly supports the role of ipdC, and thus that of the indole-3-pyruvic acid pathway, in IAA biosynthesis by strain 299R in situ. The plant induction and apparent regulation of ipdC by low water availability indicate that this gene, and presumably IAA synthesis, are involved in a response to conditions encountered by E. herbicola in its natural habitat on leaves. |
| first_indexed | 2024-04-13T04:09:04Z |
| format | Article |
| id | doaj.art-9e806b6b9ebc4137af92f14a25a23aab |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:09:04Z |
| publishDate | 1997-05-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-9e806b6b9ebc4137af92f14a25a23aab2022-12-22T03:03:10ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77061997-05-0110449950510.1094/MPMI.1997.10.4.499Environmental Signals Modulate the Expression of an Indole-3-Acetic Acid Biosynthetic Gene in Erwinia herbicolaMaria T. BrandlSteven E. LindowThe ipdC gene of Erwinia herbicola strain 299R encodes an indolepyruvate decarboxylase involved in the biosynthesis of indole-3-acetic acid (IAA). Transcriptional fusions of ipdC to an ice nucleation reporter gene (inaZ) were used to study the expression of ipdC in vitro and in situ on plants. ipdC was expressed only at low levels in liquid media and independently of factors such as richness of the medium, pH, nitrogen availability, the presence of l-tryptophan or oxygen, and growth phase of the culture. However, the transcriptional activity of ipdC increased approximately 18-fold under low solute and matric potentials in culture. ipdC was also induced 32-fold on leaves of bean and tobacco and 1,000-fold on pear flowers. This is the first report of the plant-inducible transcription of a bacterial IAA biosynthetic gene. It strongly supports the role of ipdC, and thus that of the indole-3-pyruvic acid pathway, in IAA biosynthesis by strain 299R in situ. The plant induction and apparent regulation of ipdC by low water availability indicate that this gene, and presumably IAA synthesis, are involved in a response to conditions encountered by E. herbicola in its natural habitat on leaves.https://apsjournals.apsnet.org/doi/10.1094/MPMI.1997.10.4.499auxinepiphyteosmoregulatedPantoea agglomeranstryptopholwater stress |
| spellingShingle | Maria T. Brandl Steven E. Lindow Environmental Signals Modulate the Expression of an Indole-3-Acetic Acid Biosynthetic Gene in Erwinia herbicola Molecular Plant-Microbe Interactions auxin epiphyte osmoregulated Pantoea agglomerans tryptophol water stress |
| title | Environmental Signals Modulate the Expression of an Indole-3-Acetic Acid Biosynthetic Gene in Erwinia herbicola |
| title_full | Environmental Signals Modulate the Expression of an Indole-3-Acetic Acid Biosynthetic Gene in Erwinia herbicola |
| title_fullStr | Environmental Signals Modulate the Expression of an Indole-3-Acetic Acid Biosynthetic Gene in Erwinia herbicola |
| title_full_unstemmed | Environmental Signals Modulate the Expression of an Indole-3-Acetic Acid Biosynthetic Gene in Erwinia herbicola |
| title_short | Environmental Signals Modulate the Expression of an Indole-3-Acetic Acid Biosynthetic Gene in Erwinia herbicola |
| title_sort | environmental signals modulate the expression of an indole 3 acetic acid biosynthetic gene in erwinia herbicola |
| topic | auxin epiphyte osmoregulated Pantoea agglomerans tryptophol water stress |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1997.10.4.499 |
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