Inhibitory effects of different fractions of Nepeta satureioides on melanin synthesis through reducing oxidative stress
Nepeta satureioides Boiss. has been used in traditional medicine of eastern countries and is famous for its medicinal properties. The aim of this study was to evaluate the effect of methanol (MeOH), n-hexane and dichloromethane (CH2Cl2) fractions of the extract on melanin synthesis and oxidative str...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2017-01-01
|
| Series: | Research in Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2017;volume=12;issue=2;spage=160;epage=167;aulast=Emami |
| _version_ | 1818656423877279744 |
|---|---|
| author | Seyed Ahmad Emami Rezvan Yazdian-Robati Mohammad Sadeghi Javad Baharara Elaheh Amini Farzaneh Salek Zahra Tayarani-Najaran |
| author_facet | Seyed Ahmad Emami Rezvan Yazdian-Robati Mohammad Sadeghi Javad Baharara Elaheh Amini Farzaneh Salek Zahra Tayarani-Najaran |
| author_sort | Seyed Ahmad Emami |
| collection | DOAJ |
| description | Nepeta satureioides Boiss. has been used in traditional medicine of eastern countries and is famous for its medicinal properties. The aim of this study was to evaluate the effect of methanol (MeOH), n-hexane and dichloromethane (CH2Cl2) fractions of the extract on melanin synthesis and oxidative stress in B16F10 melanoma cell line. The B16F10 cell line viability after treatment with increasing concentrations of different fractions of the plant (5-60 μg/mL) was measured using MTT assay. The inhibitory effect on synthesis of melanin, mushroom tyrosinase activity, cellular tyrosinase and oxidative stress were determined by the colorimetric and fluorometric methods. The data showed that at concentrations below 60 μg/mL, fractions did not show significant toxicity on melanoma cells. The amount of melanin synthesis by MeOH and CH2Cl2 fractions and mushroom tyrosinase activity by the MeOH fraction declined in B16F10 cells. In addition to the capacity of MeOH, n- hexane and CH2Cl2 fractions in decreasing the amount of reactive oxygen species (ROS) in melanoma cells, all fractions revealed remarkable antioxidant activity. The melanogenesis inhibitory and antioxidant effects of N. satureioides on B16F10 cells may suggest this plant as a new pharmaceutical agent in reducing skin pigment and skin aging in cosmetic industry. |
| first_indexed | 2024-12-17T03:25:21Z |
| format | Article |
| id | doaj.art-9e841b89a399481ba508190129c26131 |
| institution | Directory Open Access Journal |
| issn | 1735-5362 1735-9414 |
| language | English |
| last_indexed | 2024-12-17T03:25:21Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Research in Pharmaceutical Sciences |
| spelling | doaj.art-9e841b89a399481ba508190129c261312022-12-21T22:05:24ZengWolters Kluwer Medknow PublicationsResearch in Pharmaceutical Sciences1735-53621735-94142017-01-0112216016710.4103/1735-5362.202455Inhibitory effects of different fractions of Nepeta satureioides on melanin synthesis through reducing oxidative stressSeyed Ahmad EmamiRezvan Yazdian-RobatiMohammad SadeghiJavad BahararaElaheh AminiFarzaneh SalekZahra Tayarani-NajaranNepeta satureioides Boiss. has been used in traditional medicine of eastern countries and is famous for its medicinal properties. The aim of this study was to evaluate the effect of methanol (MeOH), n-hexane and dichloromethane (CH2Cl2) fractions of the extract on melanin synthesis and oxidative stress in B16F10 melanoma cell line. The B16F10 cell line viability after treatment with increasing concentrations of different fractions of the plant (5-60 μg/mL) was measured using MTT assay. The inhibitory effect on synthesis of melanin, mushroom tyrosinase activity, cellular tyrosinase and oxidative stress were determined by the colorimetric and fluorometric methods. The data showed that at concentrations below 60 μg/mL, fractions did not show significant toxicity on melanoma cells. The amount of melanin synthesis by MeOH and CH2Cl2 fractions and mushroom tyrosinase activity by the MeOH fraction declined in B16F10 cells. In addition to the capacity of MeOH, n- hexane and CH2Cl2 fractions in decreasing the amount of reactive oxygen species (ROS) in melanoma cells, all fractions revealed remarkable antioxidant activity. The melanogenesis inhibitory and antioxidant effects of N. satureioides on B16F10 cells may suggest this plant as a new pharmaceutical agent in reducing skin pigment and skin aging in cosmetic industry.http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2017;volume=12;issue=2;spage=160;epage=167;aulast=Emamimelanogenesis; tyrosinase; melanoma; nepeta satureioides boiss; lamiaceae |
| spellingShingle | Seyed Ahmad Emami Rezvan Yazdian-Robati Mohammad Sadeghi Javad Baharara Elaheh Amini Farzaneh Salek Zahra Tayarani-Najaran Inhibitory effects of different fractions of Nepeta satureioides on melanin synthesis through reducing oxidative stress Research in Pharmaceutical Sciences melanogenesis; tyrosinase; melanoma; nepeta satureioides boiss; lamiaceae |
| title | Inhibitory effects of different fractions of Nepeta satureioides on melanin synthesis through reducing oxidative stress |
| title_full | Inhibitory effects of different fractions of Nepeta satureioides on melanin synthesis through reducing oxidative stress |
| title_fullStr | Inhibitory effects of different fractions of Nepeta satureioides on melanin synthesis through reducing oxidative stress |
| title_full_unstemmed | Inhibitory effects of different fractions of Nepeta satureioides on melanin synthesis through reducing oxidative stress |
| title_short | Inhibitory effects of different fractions of Nepeta satureioides on melanin synthesis through reducing oxidative stress |
| title_sort | inhibitory effects of different fractions of nepeta satureioides on melanin synthesis through reducing oxidative stress |
| topic | melanogenesis; tyrosinase; melanoma; nepeta satureioides boiss; lamiaceae |
| url | http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2017;volume=12;issue=2;spage=160;epage=167;aulast=Emami |
| work_keys_str_mv | AT seyedahmademami inhibitoryeffectsofdifferentfractionsofnepetasatureioidesonmelaninsynthesisthroughreducingoxidativestress AT rezvanyazdianrobati inhibitoryeffectsofdifferentfractionsofnepetasatureioidesonmelaninsynthesisthroughreducingoxidativestress AT mohammadsadeghi inhibitoryeffectsofdifferentfractionsofnepetasatureioidesonmelaninsynthesisthroughreducingoxidativestress AT javadbaharara inhibitoryeffectsofdifferentfractionsofnepetasatureioidesonmelaninsynthesisthroughreducingoxidativestress AT elahehamini inhibitoryeffectsofdifferentfractionsofnepetasatureioidesonmelaninsynthesisthroughreducingoxidativestress AT farzanehsalek inhibitoryeffectsofdifferentfractionsofnepetasatureioidesonmelaninsynthesisthroughreducingoxidativestress AT zahratayaraninajaran inhibitoryeffectsofdifferentfractionsofnepetasatureioidesonmelaninsynthesisthroughreducingoxidativestress |