Features of immune status in different states of uric acid metabolism in female rats
<p class="pre" style="text-align: justify; text-justify: inter-ideograph; background: #f8f9fa;" align="justify"><strong><span style="font-family: 'Times New Roman';">Background.</span></strong><span style="mso-sp...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Kazimierz Wielki University
2019-12-01
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| Series: | Journal of Education, Health and Sport |
| Subjects: | |
| Online Access: | https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/28888 |
| _version_ | 1818602361929596928 |
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| author | Anatoliy Gozhenko Vadym Smagliy Inna Korda Nataliya Badiuk Walery Zukow Igor Popovych |
| author_facet | Anatoliy Gozhenko Vadym Smagliy Inna Korda Nataliya Badiuk Walery Zukow Igor Popovych |
| author_sort | Anatoliy Gozhenko |
| collection | DOAJ |
| description | <p class="pre" style="text-align: justify; text-justify: inter-ideograph; background: #f8f9fa;" align="justify"><strong><span style="font-family: 'Times New Roman';">Background.</span></strong><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 10,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">Previously, we </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">found a wide range of uric acid metabolism parameters</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">and </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">functional relationships of uricemia and uricosuria with the parameters of immunity in </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">healthy</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> female rats analyzed.</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;"> It was found by canonical correlation analysis that both parameters of uric acid metabolism determine the immunity status of rats by 71%</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">.</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">The purpose of this study is to determine the features of immune status in rats with quantitatively and qualitatively different uric acid metabolism.</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><strong><span style="font-family: 'Times New Roman';">Material and Methods</span></strong><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">. Experiment was performed on 60 healthy female Wistar rats 220-300 g. Among them 10 animals remained intact, using tap water from drinking ad libitum. The rats of others groups for 6 days administered through the tube various fluids. The serum and urine levels of the uric acid (uricase method) were determined</span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt; background: #ffffff; mso-shading: #ffffff;">. </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">In the blood, the parameters of immun</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">ity</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> were determined</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">. From thymus and spleen made smears-imprints for counting splenocytogram and thymocytogram. For them, as well as immunocytogram and leukocytogram of blood, Shannon’s entropy was calculated.</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;"> </span><strong><span style="font-family: 'Times New Roman'; color: #222222;">Results.</span></strong><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">Screening of immune profiles of four quantitative and qualitative uric acid metabolism clusters revealed 6 functional-metabolic patterns, two of which are quasilinear (</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">enhanc</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">ing and suppressing), with immune extremes at moderately elevated uricemia, two U-shaped with immune optimum at moderately reduced uricemia levels, as well as a non-reactive pattern. Discriminant analysis revealed 14 parameters of immunity, the constellation of which four clusters of metabolism of uric acid clearly distinguish. </span><strong><span style="font-family: 'Times New Roman'; color: #222222;">Conclusion</span></strong><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">.</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 10,0000pt;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">Endogenous</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">uric</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">acid</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">exerts</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">a</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">modulatory</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">immunotropic</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">effect</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">in</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">healthy</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">female</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">rats</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">.</span></p> |
| first_indexed | 2024-12-16T13:06:04Z |
| format | Article |
| id | doaj.art-9e862e3945944f3581a5589d40111f51 |
| institution | Directory Open Access Journal |
| issn | 2391-8306 |
| language | English |
| last_indexed | 2024-12-16T13:06:04Z |
| publishDate | 2019-12-01 |
| publisher | Kazimierz Wielki University |
| record_format | Article |
| series | Journal of Education, Health and Sport |
| spelling | doaj.art-9e862e3945944f3581a5589d40111f512022-12-21T22:30:45ZengKazimierz Wielki UniversityJournal of Education, Health and Sport2391-83062019-12-0191216718010.12775/JEHS.2019.09.12.01923720Features of immune status in different states of uric acid metabolism in female ratsAnatoliy Gozhenko0Vadym Smagliy1Inna Korda2Nataliya Badiuk3Walery Zukow4Igor Popovych5Ukrainian Scientific Research Institute of Medicine for Transport, OdesaUkrainian Scientific Research Institute of Medicine for Transport, OdesaIY Horbachevs’kyi National Medical University, Ternopil’Ukrainian Scientific Research Institute of Medicine for Transport, OdesaNicolaus Copernicus University, TorunOO Bohomolets’ Institute of Physiology, Kyїv<p class="pre" style="text-align: justify; text-justify: inter-ideograph; background: #f8f9fa;" align="justify"><strong><span style="font-family: 'Times New Roman';">Background.</span></strong><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 10,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">Previously, we </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">found a wide range of uric acid metabolism parameters</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">and </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">functional relationships of uricemia and uricosuria with the parameters of immunity in </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">healthy</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> female rats analyzed.</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;"> It was found by canonical correlation analysis that both parameters of uric acid metabolism determine the immunity status of rats by 71%</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">.</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">The purpose of this study is to determine the features of immune status in rats with quantitatively and qualitatively different uric acid metabolism.</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><strong><span style="font-family: 'Times New Roman';">Material and Methods</span></strong><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">. Experiment was performed on 60 healthy female Wistar rats 220-300 g. Among them 10 animals remained intact, using tap water from drinking ad libitum. The rats of others groups for 6 days administered through the tube various fluids. The serum and urine levels of the uric acid (uricase method) were determined</span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt; background: #ffffff; mso-shading: #ffffff;">. </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">In the blood, the parameters of immun</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">ity</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> were determined</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">. From thymus and spleen made smears-imprints for counting splenocytogram and thymocytogram. For them, as well as immunocytogram and leukocytogram of blood, Shannon’s entropy was calculated.</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;"> </span><strong><span style="font-family: 'Times New Roman'; color: #222222;">Results.</span></strong><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">Screening of immune profiles of four quantitative and qualitative uric acid metabolism clusters revealed 6 functional-metabolic patterns, two of which are quasilinear (</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">enhanc</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">ing and suppressing), with immune extremes at moderately elevated uricemia, two U-shaped with immune optimum at moderately reduced uricemia levels, as well as a non-reactive pattern. Discriminant analysis revealed 14 parameters of immunity, the constellation of which four clusters of metabolism of uric acid clearly distinguish. </span><strong><span style="font-family: 'Times New Roman'; color: #222222;">Conclusion</span></strong><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">.</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 10,0000pt;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">Endogenous</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">uric</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">acid</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">exerts</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">a</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">modulatory</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">immunotropic</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">effect</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">in</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">healthy</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">female</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">rats</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">.</span></p>https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/28888uricosuriauricemiaimmunityrelationshipsfemale rats. |
| spellingShingle | Anatoliy Gozhenko Vadym Smagliy Inna Korda Nataliya Badiuk Walery Zukow Igor Popovych Features of immune status in different states of uric acid metabolism in female rats Journal of Education, Health and Sport uricosuria uricemia immunity relationships female rats. |
| title | Features of immune status in different states of uric acid metabolism in female rats |
| title_full | Features of immune status in different states of uric acid metabolism in female rats |
| title_fullStr | Features of immune status in different states of uric acid metabolism in female rats |
| title_full_unstemmed | Features of immune status in different states of uric acid metabolism in female rats |
| title_short | Features of immune status in different states of uric acid metabolism in female rats |
| title_sort | features of immune status in different states of uric acid metabolism in female rats |
| topic | uricosuria uricemia immunity relationships female rats. |
| url | https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/28888 |
| work_keys_str_mv | AT anatoliygozhenko featuresofimmunestatusindifferentstatesofuricacidmetabolisminfemalerats AT vadymsmagliy featuresofimmunestatusindifferentstatesofuricacidmetabolisminfemalerats AT innakorda featuresofimmunestatusindifferentstatesofuricacidmetabolisminfemalerats AT nataliyabadiuk featuresofimmunestatusindifferentstatesofuricacidmetabolisminfemalerats AT waleryzukow featuresofimmunestatusindifferentstatesofuricacidmetabolisminfemalerats AT igorpopovych featuresofimmunestatusindifferentstatesofuricacidmetabolisminfemalerats |