NT5E mutations that cause human disease are associated with intracellular mistrafficking of NT5E protein.
Ecto-5'-nucleotidase/CD73/NT5E, the product of the NT5E gene, is the dominant enzyme in the generation of adenosine from degradation of AMP in the extracellular environment. Nonsense (c.662C→A, p.S221X designated F1, c.1609dupA, p.V537fsX7 designated F3) and missense (c.1073G→A, p.C358Y designa...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4041762?pdf=render |
| _version_ | 1828376474917273600 |
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| author | Michel Fausther Elise G Lavoie Jessica R Goree Giulia Baldini Jonathan A Dranoff |
| author_facet | Michel Fausther Elise G Lavoie Jessica R Goree Giulia Baldini Jonathan A Dranoff |
| author_sort | Michel Fausther |
| collection | DOAJ |
| description | Ecto-5'-nucleotidase/CD73/NT5E, the product of the NT5E gene, is the dominant enzyme in the generation of adenosine from degradation of AMP in the extracellular environment. Nonsense (c.662C→A, p.S221X designated F1, c.1609dupA, p.V537fsX7 designated F3) and missense (c.1073G→A, p.C358Y designated F2) NT5E gene mutations in three distinct families have been shown recently to cause premature arterial calcification disease in human patients. However, the underlying mechanisms by which loss-of-function NT5E mutations cause human disease are unknown. We hypothesized that human NT5E gene mutations cause mistrafficking of the defective proteins within cells, ultimately blocking NT5E catalytic function. To test this hypothesis, plasmids encoding cDNAs of wild type and mutant human NT5E tagged with the fluorescent probe DsRed were generated and used for transfection and heterologous expression in immortalized monkey COS-7 kidney cells that lack native NT5E protein. Enzyme histochemistry and Malachite green assays were performed to assess the biochemical activities of wild type and mutant fusion NT5E proteins. Subcellular trafficking of fusion NT5E proteins was monitored by confocal microscopy and western blot analysis of fractionated cell constituents. All 3 F1, F2, and F3 mutations result in a protein with significantly reduced trafficking to the plasma membrane and reduced ER retention as compared to wild type protein. Confocal immunofluorescence demonstrates vesicles containing DsRed-tagged NT5E proteins (F1, F2 and F3) in the cell synthetic apparatus. All 3 mutations resulted in absent NT5E enzymatic activity at the cell surface. In conclusion, three familial NT5E mutations (F1, F2, F3) result in novel trafficking defects associated with human disease. These novel genetic causes of human disease suggest that the syndrome of premature arterial calcification due to NT5E mutations may also involve a novel "trafficking-opathy". |
| first_indexed | 2024-04-14T08:00:28Z |
| format | Article |
| id | doaj.art-9e8abbcee1a44dbca4932e27ae3563b2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T08:00:28Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9e8abbcee1a44dbca4932e27ae3563b22022-12-22T02:04:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0196e9856810.1371/journal.pone.0098568NT5E mutations that cause human disease are associated with intracellular mistrafficking of NT5E protein.Michel FaustherElise G LavoieJessica R GoreeGiulia BaldiniJonathan A DranoffEcto-5'-nucleotidase/CD73/NT5E, the product of the NT5E gene, is the dominant enzyme in the generation of adenosine from degradation of AMP in the extracellular environment. Nonsense (c.662C→A, p.S221X designated F1, c.1609dupA, p.V537fsX7 designated F3) and missense (c.1073G→A, p.C358Y designated F2) NT5E gene mutations in three distinct families have been shown recently to cause premature arterial calcification disease in human patients. However, the underlying mechanisms by which loss-of-function NT5E mutations cause human disease are unknown. We hypothesized that human NT5E gene mutations cause mistrafficking of the defective proteins within cells, ultimately blocking NT5E catalytic function. To test this hypothesis, plasmids encoding cDNAs of wild type and mutant human NT5E tagged with the fluorescent probe DsRed were generated and used for transfection and heterologous expression in immortalized monkey COS-7 kidney cells that lack native NT5E protein. Enzyme histochemistry and Malachite green assays were performed to assess the biochemical activities of wild type and mutant fusion NT5E proteins. Subcellular trafficking of fusion NT5E proteins was monitored by confocal microscopy and western blot analysis of fractionated cell constituents. All 3 F1, F2, and F3 mutations result in a protein with significantly reduced trafficking to the plasma membrane and reduced ER retention as compared to wild type protein. Confocal immunofluorescence demonstrates vesicles containing DsRed-tagged NT5E proteins (F1, F2 and F3) in the cell synthetic apparatus. All 3 mutations resulted in absent NT5E enzymatic activity at the cell surface. In conclusion, three familial NT5E mutations (F1, F2, F3) result in novel trafficking defects associated with human disease. These novel genetic causes of human disease suggest that the syndrome of premature arterial calcification due to NT5E mutations may also involve a novel "trafficking-opathy".http://europepmc.org/articles/PMC4041762?pdf=render |
| spellingShingle | Michel Fausther Elise G Lavoie Jessica R Goree Giulia Baldini Jonathan A Dranoff NT5E mutations that cause human disease are associated with intracellular mistrafficking of NT5E protein. PLoS ONE |
| title | NT5E mutations that cause human disease are associated with intracellular mistrafficking of NT5E protein. |
| title_full | NT5E mutations that cause human disease are associated with intracellular mistrafficking of NT5E protein. |
| title_fullStr | NT5E mutations that cause human disease are associated with intracellular mistrafficking of NT5E protein. |
| title_full_unstemmed | NT5E mutations that cause human disease are associated with intracellular mistrafficking of NT5E protein. |
| title_short | NT5E mutations that cause human disease are associated with intracellular mistrafficking of NT5E protein. |
| title_sort | nt5e mutations that cause human disease are associated with intracellular mistrafficking of nt5e protein |
| url | http://europepmc.org/articles/PMC4041762?pdf=render |
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