Marine Seagrass Extract of <i>Thalassia testudinum</i> Suppresses Colorectal Tumor Growth, Motility and Angiogenesis by Autophagic Stress and Immunogenic Cell Death Pathways
Marine plants have become an inexhaustible reservoir of new phytopharmaceuticals for cancer treatment. We demonstrate in vitro/in vivo antitumor efficacy of a standardized polyphenol extract from the marine angiosperm <i>Thalassia testudinum</i> (TTE) in colon tumor cell lines (RKO, SW48...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/19/2/52 |
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| author | Ivones Hernández-Balmaseda Idania Rodeiro Guerra Ken Declerck José Alfredo Herrera Isidrón Claudina Pérez-Novo Guy Van Camp Olivier De Wever Kethia González Mayrel Labrada Adriana Carr Geovanni Dantas-Cassali Diego Carlos dos Reis Livan Delgado-Roche Roberto Rafael Nuñez René Delgado-Hernández Miguel David Fernández Miriam T. Paz-Lopes Wim Vanden Berghe |
| author_facet | Ivones Hernández-Balmaseda Idania Rodeiro Guerra Ken Declerck José Alfredo Herrera Isidrón Claudina Pérez-Novo Guy Van Camp Olivier De Wever Kethia González Mayrel Labrada Adriana Carr Geovanni Dantas-Cassali Diego Carlos dos Reis Livan Delgado-Roche Roberto Rafael Nuñez René Delgado-Hernández Miguel David Fernández Miriam T. Paz-Lopes Wim Vanden Berghe |
| author_sort | Ivones Hernández-Balmaseda |
| collection | DOAJ |
| description | Marine plants have become an inexhaustible reservoir of new phytopharmaceuticals for cancer treatment. We demonstrate in vitro/in vivo antitumor efficacy of a standardized polyphenol extract from the marine angiosperm <i>Thalassia testudinum</i> (TTE) in colon tumor cell lines (RKO, SW480, and CT26) and a syngeneic allograft murine colorectal cancer model. MTT assays revealed a dose-dependent decrease of cell viability of RKO, CT26, and SW480 cells upon TTE treatment with IC<sub>50</sub> values of, respectively, 175, 115, and 60 μg/mL. Furthermore, TTE significantly prevented basal and bFGF-induced angiogenesis in the chicken chorioallantoic membrane angiogenesis assay. In addition, TTE suppressed bFGF-induced migration of endothelial cells in a wound closure assay. Finally, TTE treatment abrogated CT26 colorectal cancer growth and increased overall organism survival in a syngeneic murine allograft model. Corresponding transcriptome profiling and pathway analysis allowed for the identification of the mechanism of action for the antitumor effects of TTE. In line with our in vitro/in vivo results, TTE treatment triggers ATF4-P53-NFκB specific gene expression and autophagy stress pathways. This results in suppression of colon cancer cell growth, cell motility, and angiogenesis pathways in vitro and in addition promotes antitumor immunogenic cell death in vivo. |
| first_indexed | 2024-03-09T03:58:41Z |
| format | Article |
| id | doaj.art-9e8ac325380a4609b27730226ac959bd |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T03:58:41Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-9e8ac325380a4609b27730226ac959bd2023-12-03T14:16:30ZengMDPI AGMarine Drugs1660-33972021-01-011925210.3390/md19020052Marine Seagrass Extract of <i>Thalassia testudinum</i> Suppresses Colorectal Tumor Growth, Motility and Angiogenesis by Autophagic Stress and Immunogenic Cell Death PathwaysIvones Hernández-Balmaseda0Idania Rodeiro Guerra1Ken Declerck2José Alfredo Herrera Isidrón3Claudina Pérez-Novo4Guy Van Camp5Olivier De Wever6Kethia González7Mayrel Labrada8Adriana Carr9Geovanni Dantas-Cassali10Diego Carlos dos Reis11Livan Delgado-Roche12Roberto Rafael Nuñez13René Delgado-Hernández14Miguel David Fernández15Miriam T. Paz-Lopes16Wim Vanden Berghe17Instituto de Ciencias del Mar (ICIMAR), Calle Loma #14 e/35 y 37, Alturas del Vedado, Plaza de la Revolución, Havana 10400, CubaInstituto de Ciencias del Mar (ICIMAR), Calle Loma #14 e/35 y 37, Alturas del Vedado, Plaza de la Revolución, Havana 10400, CubaLaboratory of Protein Science, Proteomics and Epigenetic Signaling (PPES) and Integrated Personalized and Precision Oncology Network (IPPON), Department of Biomedical Sciences, Campus Drie Eiken, University of Antwerp, Building S, 4th Floor, Universiteitsplein 1, 2610 Wilrijk, BelgiumInstituto de Ciencia y Tecnología de Materiales (IMRE), Universidad de la Habana, Zapata y G, Vedado, Plaza de la Revolución, Havana 10400, CubaLaboratory of Protein Science, Proteomics and Epigenetic Signaling (PPES) and Integrated Personalized and Precision Oncology Network (IPPON), Department of Biomedical Sciences, Campus Drie Eiken, University of Antwerp, Building S, 4th Floor, Universiteitsplein 1, 2610 Wilrijk, BelgiumCenter of Medical Genetics, University of Antwerp and Antwerp University Hospital, Prins Boudewijnlaan 43, 2650 Edegem, BelgiumLaboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Cancer Research Institute Ghent (CRIG), UZ-Gent, 9000 Gent, BelgiumInstituto de Ciencias del Mar (ICIMAR), Calle Loma #14 e/35 y 37, Alturas del Vedado, Plaza de la Revolución, Havana 10400, CubaCenter of Molecular Immunology, Calle 17, Atabey, Playa, Havana 11300, CubaCenter of Molecular Immunology, Calle 17, Atabey, Playa, Havana 11300, CubaInstitute of Biological Sciences (ICB), Federal University of Minas Gerais (UFMG), Belo Horizonte 31207-901, BrazilInstitute of Biological Sciences (ICB), Federal University of Minas Gerais (UFMG), Belo Horizonte 31207-901, BrazilInstituto de Ciencias del Mar (ICIMAR), Calle Loma #14 e/35 y 37, Alturas del Vedado, Plaza de la Revolución, Havana 10400, CubaInstituto de Ciencias del Mar (ICIMAR), Calle Loma #14 e/35 y 37, Alturas del Vedado, Plaza de la Revolución, Havana 10400, CubaInstituto de Farmacia y Alimentos (IFAL), Universidad de La Habana, (UH), Ave. 23 # 21425 Entre 214 and 222, La Coronela, La Lisa, Havana 13600, CubaInstituto de Ciencias del Mar (ICIMAR), Calle Loma #14 e/35 y 37, Alturas del Vedado, Plaza de la Revolución, Havana 10400, CubaInstitute of Biological Sciences (ICB), Federal University of Minas Gerais (UFMG), Belo Horizonte 31207-901, BrazilLaboratory of Protein Science, Proteomics and Epigenetic Signaling (PPES) and Integrated Personalized and Precision Oncology Network (IPPON), Department of Biomedical Sciences, Campus Drie Eiken, University of Antwerp, Building S, 4th Floor, Universiteitsplein 1, 2610 Wilrijk, BelgiumMarine plants have become an inexhaustible reservoir of new phytopharmaceuticals for cancer treatment. We demonstrate in vitro/in vivo antitumor efficacy of a standardized polyphenol extract from the marine angiosperm <i>Thalassia testudinum</i> (TTE) in colon tumor cell lines (RKO, SW480, and CT26) and a syngeneic allograft murine colorectal cancer model. MTT assays revealed a dose-dependent decrease of cell viability of RKO, CT26, and SW480 cells upon TTE treatment with IC<sub>50</sub> values of, respectively, 175, 115, and 60 μg/mL. Furthermore, TTE significantly prevented basal and bFGF-induced angiogenesis in the chicken chorioallantoic membrane angiogenesis assay. In addition, TTE suppressed bFGF-induced migration of endothelial cells in a wound closure assay. Finally, TTE treatment abrogated CT26 colorectal cancer growth and increased overall organism survival in a syngeneic murine allograft model. Corresponding transcriptome profiling and pathway analysis allowed for the identification of the mechanism of action for the antitumor effects of TTE. In line with our in vitro/in vivo results, TTE treatment triggers ATF4-P53-NFκB specific gene expression and autophagy stress pathways. This results in suppression of colon cancer cell growth, cell motility, and angiogenesis pathways in vitro and in addition promotes antitumor immunogenic cell death in vivo.https://www.mdpi.com/1660-3397/19/2/52<i>Thalassia testudinum</i>cytotoxicityantitumoranti-angiogenicgene expression |
| spellingShingle | Ivones Hernández-Balmaseda Idania Rodeiro Guerra Ken Declerck José Alfredo Herrera Isidrón Claudina Pérez-Novo Guy Van Camp Olivier De Wever Kethia González Mayrel Labrada Adriana Carr Geovanni Dantas-Cassali Diego Carlos dos Reis Livan Delgado-Roche Roberto Rafael Nuñez René Delgado-Hernández Miguel David Fernández Miriam T. Paz-Lopes Wim Vanden Berghe Marine Seagrass Extract of <i>Thalassia testudinum</i> Suppresses Colorectal Tumor Growth, Motility and Angiogenesis by Autophagic Stress and Immunogenic Cell Death Pathways Marine Drugs <i>Thalassia testudinum</i> cytotoxicity antitumor anti-angiogenic gene expression |
| title | Marine Seagrass Extract of <i>Thalassia testudinum</i> Suppresses Colorectal Tumor Growth, Motility and Angiogenesis by Autophagic Stress and Immunogenic Cell Death Pathways |
| title_full | Marine Seagrass Extract of <i>Thalassia testudinum</i> Suppresses Colorectal Tumor Growth, Motility and Angiogenesis by Autophagic Stress and Immunogenic Cell Death Pathways |
| title_fullStr | Marine Seagrass Extract of <i>Thalassia testudinum</i> Suppresses Colorectal Tumor Growth, Motility and Angiogenesis by Autophagic Stress and Immunogenic Cell Death Pathways |
| title_full_unstemmed | Marine Seagrass Extract of <i>Thalassia testudinum</i> Suppresses Colorectal Tumor Growth, Motility and Angiogenesis by Autophagic Stress and Immunogenic Cell Death Pathways |
| title_short | Marine Seagrass Extract of <i>Thalassia testudinum</i> Suppresses Colorectal Tumor Growth, Motility and Angiogenesis by Autophagic Stress and Immunogenic Cell Death Pathways |
| title_sort | marine seagrass extract of i thalassia testudinum i suppresses colorectal tumor growth motility and angiogenesis by autophagic stress and immunogenic cell death pathways |
| topic | <i>Thalassia testudinum</i> cytotoxicity antitumor anti-angiogenic gene expression |
| url | https://www.mdpi.com/1660-3397/19/2/52 |
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