TCF12 Deficiency Impairs the Proliferation of Glioblastoma Tumor Cells and Improves Survival
Isocitrate dehydrogenase (IDH)-wild-type glioblastoma (GBM) is the most common and aggressive primary brain tumor which carries a very poor overall prognosis and is universally fatal. Understanding the transcriptional regulation of the proliferation of GBM tumor cells is critical for developing nove...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/15/7/2033 |
| _version_ | 1797608261491359744 |
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| author | Yunong Pang Sichang Zhou Paul Zumbo Doron Betel Babacar Cisse |
| author_facet | Yunong Pang Sichang Zhou Paul Zumbo Doron Betel Babacar Cisse |
| author_sort | Yunong Pang |
| collection | DOAJ |
| description | Isocitrate dehydrogenase (IDH)-wild-type glioblastoma (GBM) is the most common and aggressive primary brain tumor which carries a very poor overall prognosis and is universally fatal. Understanding the transcriptional regulation of the proliferation of GBM tumor cells is critical for developing novel and effective treatments. In this study, we investigate the role of the transcription factor TCF12 in the regulation of GBM proliferation using human and murine GBM cell lines and an in vivo GBM xenograft model. Our study shows that TCF12 deficiency severely impairs proliferation of tumor cells in vitro by disrupting/blocking the G1 to S phase transition. We also discover that TCF12 loss significantly improves animal survival and that TCF12-deficient tumors grow much slower in vivo. Overexpression of TCF12, on the other hand, leads to an increase in the proliferation of tumor cells in vitro and more aggressive tumor progression in vivo. Interestingly, loss of TCF12 leads to upregulation of signature genes of the oligodendrocytic lineage in GBM stem cells, suggesting a role for TCF12 in inhibiting differentiation along the oligodendrocytic lineage. Transcriptomic data also reveals that loss of TCF12 leads to dysregulation of the expression of key genes in the cell cycle. Our work demonstrates critical roles of TCF12 in GBM tumor progression. |
| first_indexed | 2024-03-11T05:41:07Z |
| format | Article |
| id | doaj.art-9e8d1802799f4b2895890c7b29d05e61 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-11T05:41:07Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-9e8d1802799f4b2895890c7b29d05e612023-11-17T16:25:06ZengMDPI AGCancers2072-66942023-03-01157203310.3390/cancers15072033TCF12 Deficiency Impairs the Proliferation of Glioblastoma Tumor Cells and Improves SurvivalYunong Pang0Sichang Zhou1Paul Zumbo2Doron Betel3Babacar Cisse4Department of Neurological Surgery, Weill Cornell Medicine, New York, NY 10065, USADepartment of Neurological Surgery, Weill Cornell Medicine, New York, NY 10065, USAInstitute of Computational Biomedicine, Weill Cornell Medicine, New York, NY 10021, USAInstitute of Computational Biomedicine, Weill Cornell Medicine, New York, NY 10021, USADepartment of Neurological Surgery, Weill Cornell Medicine, New York, NY 10065, USAIsocitrate dehydrogenase (IDH)-wild-type glioblastoma (GBM) is the most common and aggressive primary brain tumor which carries a very poor overall prognosis and is universally fatal. Understanding the transcriptional regulation of the proliferation of GBM tumor cells is critical for developing novel and effective treatments. In this study, we investigate the role of the transcription factor TCF12 in the regulation of GBM proliferation using human and murine GBM cell lines and an in vivo GBM xenograft model. Our study shows that TCF12 deficiency severely impairs proliferation of tumor cells in vitro by disrupting/blocking the G1 to S phase transition. We also discover that TCF12 loss significantly improves animal survival and that TCF12-deficient tumors grow much slower in vivo. Overexpression of TCF12, on the other hand, leads to an increase in the proliferation of tumor cells in vitro and more aggressive tumor progression in vivo. Interestingly, loss of TCF12 leads to upregulation of signature genes of the oligodendrocytic lineage in GBM stem cells, suggesting a role for TCF12 in inhibiting differentiation along the oligodendrocytic lineage. Transcriptomic data also reveals that loss of TCF12 leads to dysregulation of the expression of key genes in the cell cycle. Our work demonstrates critical roles of TCF12 in GBM tumor progression.https://www.mdpi.com/2072-6694/15/7/2033glioblastomas (GBMs)TCF12cell cycle and proliferationxenograftoligodendrocytic lineage |
| spellingShingle | Yunong Pang Sichang Zhou Paul Zumbo Doron Betel Babacar Cisse TCF12 Deficiency Impairs the Proliferation of Glioblastoma Tumor Cells and Improves Survival Cancers glioblastomas (GBMs) TCF12 cell cycle and proliferation xenograft oligodendrocytic lineage |
| title | TCF12 Deficiency Impairs the Proliferation of Glioblastoma Tumor Cells and Improves Survival |
| title_full | TCF12 Deficiency Impairs the Proliferation of Glioblastoma Tumor Cells and Improves Survival |
| title_fullStr | TCF12 Deficiency Impairs the Proliferation of Glioblastoma Tumor Cells and Improves Survival |
| title_full_unstemmed | TCF12 Deficiency Impairs the Proliferation of Glioblastoma Tumor Cells and Improves Survival |
| title_short | TCF12 Deficiency Impairs the Proliferation of Glioblastoma Tumor Cells and Improves Survival |
| title_sort | tcf12 deficiency impairs the proliferation of glioblastoma tumor cells and improves survival |
| topic | glioblastomas (GBMs) TCF12 cell cycle and proliferation xenograft oligodendrocytic lineage |
| url | https://www.mdpi.com/2072-6694/15/7/2033 |
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