Oral Administration of Achyranthis radix Extract Prevents TMA-induced Allergic Contact Dermatitis by Regulating Th2 Cytokine and Chemokine Production in Vivo
Allergic contact dermatitis (ACD) remains a major skin disease in many countries, necessitating the discovery of novel and effective anti-ACD agents. In this study, we investigated the preventive effects of Achyranthis radix extract (AcRE) on trimellitic anhydride (TMA)-induced dermatitis and the po...
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| Format: | Article |
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MDPI AG
2015-12-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/20/12/19788 |
| _version_ | 1828378207525535744 |
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| author | Sung Keun Jung Dae Woon Choi Da-Ae Kwon Min Jung Kim Ki Seung Seong Dong-Hwa Shon |
| author_facet | Sung Keun Jung Dae Woon Choi Da-Ae Kwon Min Jung Kim Ki Seung Seong Dong-Hwa Shon |
| author_sort | Sung Keun Jung |
| collection | DOAJ |
| description | Allergic contact dermatitis (ACD) remains a major skin disease in many countries, necessitating the discovery of novel and effective anti-ACD agents. In this study, we investigated the preventive effects of Achyranthis radix extract (AcRE) on trimellitic anhydride (TMA)-induced dermatitis and the potential mechanism of action involved. Oral administration of AcRE and prednisolone (PS) significantly suppressed TMA-induced increases in ear and epidermal thickness, and IgE expression. In addition, abnormal expression of IL-1β and TNF-α protein and mRNA was also significantly attenuated by oral administration of AcRE. Treatment with AcRE also significantly suppressed TMA-induced IL-4 and IL-13 cytokines and mRNA expression in vivo. Moreover, AcRE strongly suppressed TMA-induced IL-4 and IL-5 production in draining lymph nodes, as well as OVA-induced IL-4 and IL-5 expression in primary cultured splenocytes. Interestingly, AcRE suppressed IL-4-induced STAT6 phosphorylation in both primary cultured splenocytes and HaCaT cells, and TMA-induced GATA3 mRNA expression ex vivo. AcRE also suppressed TMA-mediated CCL11 and IL-4-induced CCL26 mRNA expression and infiltration of CCR3 positive cells. The major compounds from AcRE were identified as gentisic acid (0.64 ± 0.2 μg/g dry weight of AcRE), protocatechuic acid (2.69 ± 0.1 μg/g dry weight of AcRE), 4-hydroxybenzoic acid (5.59 ± 0.3 μg/g dry weight of AcRE), caffeic acid (4.21 ± 0.1 μg/g dry weight of AcRE), and ferulic acid (14.78 ± 0.4 ± 0.3 μg/g dry weight of AcRE). Taken together, these results suggest that AcRE has potential for development as an agent to prevent and treat allergic contact dermatitis. |
| first_indexed | 2024-04-14T08:22:16Z |
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| language | English |
| last_indexed | 2024-04-14T08:22:16Z |
| publishDate | 2015-12-01 |
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| series | Molecules |
| spelling | doaj.art-9e8d340550094b11b92ecab39767955d2022-12-22T02:04:10ZengMDPI AGMolecules1420-30492015-12-012012215842159610.3390/molecules201219788molecules201219788Oral Administration of Achyranthis radix Extract Prevents TMA-induced Allergic Contact Dermatitis by Regulating Th2 Cytokine and Chemokine Production in VivoSung Keun Jung0Dae Woon Choi1Da-Ae Kwon2Min Jung Kim3Ki Seung Seong4Dong-Hwa Shon5Research Group of Nutraceuticals for Metabolic Syndrome, Korea Food Research Institute, Seongnam 13539, KoreaResearch Group of Nutraceuticals for Metabolic Syndrome, Korea Food Research Institute, Seongnam 13539, KoreaResearch Group of Nutraceuticals for Metabolic Syndrome, Korea Food Research Institute, Seongnam 13539, KoreaResearch Group of Metabolic Mechanism, Korea Food Research Institute, Seongnam 13539, KoreaResearch Group of Nutraceuticals for Metabolic Syndrome, Korea Food Research Institute, Seongnam 13539, KoreaResearch Group of Nutraceuticals for Metabolic Syndrome, Korea Food Research Institute, Seongnam 13539, KoreaAllergic contact dermatitis (ACD) remains a major skin disease in many countries, necessitating the discovery of novel and effective anti-ACD agents. In this study, we investigated the preventive effects of Achyranthis radix extract (AcRE) on trimellitic anhydride (TMA)-induced dermatitis and the potential mechanism of action involved. Oral administration of AcRE and prednisolone (PS) significantly suppressed TMA-induced increases in ear and epidermal thickness, and IgE expression. In addition, abnormal expression of IL-1β and TNF-α protein and mRNA was also significantly attenuated by oral administration of AcRE. Treatment with AcRE also significantly suppressed TMA-induced IL-4 and IL-13 cytokines and mRNA expression in vivo. Moreover, AcRE strongly suppressed TMA-induced IL-4 and IL-5 production in draining lymph nodes, as well as OVA-induced IL-4 and IL-5 expression in primary cultured splenocytes. Interestingly, AcRE suppressed IL-4-induced STAT6 phosphorylation in both primary cultured splenocytes and HaCaT cells, and TMA-induced GATA3 mRNA expression ex vivo. AcRE also suppressed TMA-mediated CCL11 and IL-4-induced CCL26 mRNA expression and infiltration of CCR3 positive cells. The major compounds from AcRE were identified as gentisic acid (0.64 ± 0.2 μg/g dry weight of AcRE), protocatechuic acid (2.69 ± 0.1 μg/g dry weight of AcRE), 4-hydroxybenzoic acid (5.59 ± 0.3 μg/g dry weight of AcRE), caffeic acid (4.21 ± 0.1 μg/g dry weight of AcRE), and ferulic acid (14.78 ± 0.4 ± 0.3 μg/g dry weight of AcRE). Taken together, these results suggest that AcRE has potential for development as an agent to prevent and treat allergic contact dermatitis.http://www.mdpi.com/1420-3049/20/12/19788Achyranthis radixallergic contact dermatitisTh2 cytokineschemokinesSTAT6 |
| spellingShingle | Sung Keun Jung Dae Woon Choi Da-Ae Kwon Min Jung Kim Ki Seung Seong Dong-Hwa Shon Oral Administration of Achyranthis radix Extract Prevents TMA-induced Allergic Contact Dermatitis by Regulating Th2 Cytokine and Chemokine Production in Vivo Molecules Achyranthis radix allergic contact dermatitis Th2 cytokines chemokines STAT6 |
| title | Oral Administration of Achyranthis radix Extract Prevents TMA-induced Allergic Contact Dermatitis by Regulating Th2 Cytokine and Chemokine Production in Vivo |
| title_full | Oral Administration of Achyranthis radix Extract Prevents TMA-induced Allergic Contact Dermatitis by Regulating Th2 Cytokine and Chemokine Production in Vivo |
| title_fullStr | Oral Administration of Achyranthis radix Extract Prevents TMA-induced Allergic Contact Dermatitis by Regulating Th2 Cytokine and Chemokine Production in Vivo |
| title_full_unstemmed | Oral Administration of Achyranthis radix Extract Prevents TMA-induced Allergic Contact Dermatitis by Regulating Th2 Cytokine and Chemokine Production in Vivo |
| title_short | Oral Administration of Achyranthis radix Extract Prevents TMA-induced Allergic Contact Dermatitis by Regulating Th2 Cytokine and Chemokine Production in Vivo |
| title_sort | oral administration of achyranthis radix extract prevents tma induced allergic contact dermatitis by regulating th2 cytokine and chemokine production in vivo |
| topic | Achyranthis radix allergic contact dermatitis Th2 cytokines chemokines STAT6 |
| url | http://www.mdpi.com/1420-3049/20/12/19788 |
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