A draft map of rhesus monkey tissue proteome for biomedical research.
Though the rhesus monkey is one of the most valuable non-human primate animal models for various human diseases because of its manageable size and genetic and proteomic similarities with humans, proteomic research using rhesus monkeys still remains challenging due to the lack of a complete protein s...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4431823?pdf=render |
| _version_ | 1818874304399409152 |
|---|---|
| author | Jin-Gyun Lee Kimberly Q McKinney Yong-Yook Lee Hae-Na Chung Antonis J Pavlopoulos Kook Y Jung Woong-Ki Kim Marcelo J Kuroda David K Han Sunil Hwang |
| author_facet | Jin-Gyun Lee Kimberly Q McKinney Yong-Yook Lee Hae-Na Chung Antonis J Pavlopoulos Kook Y Jung Woong-Ki Kim Marcelo J Kuroda David K Han Sunil Hwang |
| author_sort | Jin-Gyun Lee |
| collection | DOAJ |
| description | Though the rhesus monkey is one of the most valuable non-human primate animal models for various human diseases because of its manageable size and genetic and proteomic similarities with humans, proteomic research using rhesus monkeys still remains challenging due to the lack of a complete protein sequence database and effective strategy. To investigate the most effective and high-throughput proteomic strategy, comparative data analysis was performed employing various protein databases and search engines. The UniProt databases of monkey, human, bovine, rat and mouse were used for the comparative analysis and also a universal database with all protein sequences from all available species was tested. At the same time, de novo sequencing was compared to the SEQUEST search algorithm to identify an optimal work flow for monkey proteomics. Employing the most effective strategy, proteomic profiling of monkey organs identified 3,481 proteins at 0.5% FDR from 9 male and 10 female tissues in an automated, high-throughput manner. Data are available via ProteomeXchange with identifier PXD001972. Based on the success of this alternative interpretation of MS data, the list of proteins identified from 12 organs of male and female subjects will benefit future rhesus monkey proteome research. |
| first_indexed | 2024-12-19T13:08:28Z |
| format | Article |
| id | doaj.art-9e8ebc1bbd274583893242e49d231c59 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T13:08:28Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9e8ebc1bbd274583893242e49d231c592022-12-21T20:19:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01105e012624310.1371/journal.pone.0126243A draft map of rhesus monkey tissue proteome for biomedical research.Jin-Gyun LeeKimberly Q McKinneyYong-Yook LeeHae-Na ChungAntonis J PavlopoulosKook Y JungWoong-Ki KimMarcelo J KurodaDavid K HanSunil HwangThough the rhesus monkey is one of the most valuable non-human primate animal models for various human diseases because of its manageable size and genetic and proteomic similarities with humans, proteomic research using rhesus monkeys still remains challenging due to the lack of a complete protein sequence database and effective strategy. To investigate the most effective and high-throughput proteomic strategy, comparative data analysis was performed employing various protein databases and search engines. The UniProt databases of monkey, human, bovine, rat and mouse were used for the comparative analysis and also a universal database with all protein sequences from all available species was tested. At the same time, de novo sequencing was compared to the SEQUEST search algorithm to identify an optimal work flow for monkey proteomics. Employing the most effective strategy, proteomic profiling of monkey organs identified 3,481 proteins at 0.5% FDR from 9 male and 10 female tissues in an automated, high-throughput manner. Data are available via ProteomeXchange with identifier PXD001972. Based on the success of this alternative interpretation of MS data, the list of proteins identified from 12 organs of male and female subjects will benefit future rhesus monkey proteome research.http://europepmc.org/articles/PMC4431823?pdf=render |
| spellingShingle | Jin-Gyun Lee Kimberly Q McKinney Yong-Yook Lee Hae-Na Chung Antonis J Pavlopoulos Kook Y Jung Woong-Ki Kim Marcelo J Kuroda David K Han Sunil Hwang A draft map of rhesus monkey tissue proteome for biomedical research. PLoS ONE |
| title | A draft map of rhesus monkey tissue proteome for biomedical research. |
| title_full | A draft map of rhesus monkey tissue proteome for biomedical research. |
| title_fullStr | A draft map of rhesus monkey tissue proteome for biomedical research. |
| title_full_unstemmed | A draft map of rhesus monkey tissue proteome for biomedical research. |
| title_short | A draft map of rhesus monkey tissue proteome for biomedical research. |
| title_sort | draft map of rhesus monkey tissue proteome for biomedical research |
| url | http://europepmc.org/articles/PMC4431823?pdf=render |
| work_keys_str_mv | AT jingyunlee adraftmapofrhesusmonkeytissueproteomeforbiomedicalresearch AT kimberlyqmckinney adraftmapofrhesusmonkeytissueproteomeforbiomedicalresearch AT yongyooklee adraftmapofrhesusmonkeytissueproteomeforbiomedicalresearch AT haenachung adraftmapofrhesusmonkeytissueproteomeforbiomedicalresearch AT antonisjpavlopoulos adraftmapofrhesusmonkeytissueproteomeforbiomedicalresearch AT kookyjung adraftmapofrhesusmonkeytissueproteomeforbiomedicalresearch AT woongkikim adraftmapofrhesusmonkeytissueproteomeforbiomedicalresearch AT marcelojkuroda adraftmapofrhesusmonkeytissueproteomeforbiomedicalresearch AT davidkhan adraftmapofrhesusmonkeytissueproteomeforbiomedicalresearch AT sunilhwang adraftmapofrhesusmonkeytissueproteomeforbiomedicalresearch AT jingyunlee draftmapofrhesusmonkeytissueproteomeforbiomedicalresearch AT kimberlyqmckinney draftmapofrhesusmonkeytissueproteomeforbiomedicalresearch AT yongyooklee draftmapofrhesusmonkeytissueproteomeforbiomedicalresearch AT haenachung draftmapofrhesusmonkeytissueproteomeforbiomedicalresearch AT antonisjpavlopoulos draftmapofrhesusmonkeytissueproteomeforbiomedicalresearch AT kookyjung draftmapofrhesusmonkeytissueproteomeforbiomedicalresearch AT woongkikim draftmapofrhesusmonkeytissueproteomeforbiomedicalresearch AT marcelojkuroda draftmapofrhesusmonkeytissueproteomeforbiomedicalresearch AT davidkhan draftmapofrhesusmonkeytissueproteomeforbiomedicalresearch AT sunilhwang draftmapofrhesusmonkeytissueproteomeforbiomedicalresearch |