SSR-Marker assisted evaluation of Genetic Diversity in Mungbean ( Vigna radiata (L.) Wilcezk) genotypes
ABSTRACT Mungbean (Vigna radiata (L.) Wilczek) also known as green gram is an important source of protein in the category of food legumes. In the present study, SSR marker is used to analyze the genetic diversity amongst 23 genotypes of mungbean. Out of a total of 10 primers used for SSR analysis re...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2018-06-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100400&lng=en&tlng=en |
| _version_ | 1818133970546589696 |
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| author | Gunnjeet Kaur Arunabh Joshi Devendra Jain |
| author_facet | Gunnjeet Kaur Arunabh Joshi Devendra Jain |
| author_sort | Gunnjeet Kaur |
| collection | DOAJ |
| description | ABSTRACT Mungbean (Vigna radiata (L.) Wilczek) also known as green gram is an important source of protein in the category of food legumes. In the present study, SSR marker is used to analyze the genetic diversity amongst 23 genotypes of mungbean. Out of a total of 10 primers used for SSR analysis revealed generation of 15 alleles. The number of alleles per locus ranged from one (CEDG006, CEDG010, CEDG050, CEDG088, CEDG092 and CEDG232) to three (CEDG 214), with an average of 1.5 allele per primer. The index for expected heterozygosity was 0.29 ranging from 0.15 to 0.49 revealed a deficit in heterozygosity. The size of amplification products varied in case of each primer and the range was found to be 100 bp to 190 bp. 13 out of 15 alleles were found polymorphic. The average PIC value of SSR marker was found to be 0.205. The value of Jaccard’s similarity coefficient had ranged from 0.28-1.00 with an average value of 0.64. The dendrogram constructed on SSR molecular marker data through UPGMA method and PCA using average linkage, had enabled grouping of the genotypes into three main clusters. Clustering pattern based on SSR marker data clearly indicated the narrow genetic base of mungbean genotypes that emphasizes the need to explore and exploit more number of germplasm from additional source to study genetic variation in mungbean for genetic improvement. The results indicated the marked usefulness of SSR in the assessment of genetic diversity in mungbean crop. |
| first_indexed | 2024-12-11T09:01:11Z |
| format | Article |
| id | doaj.art-9e8f1c7bfb1c492e91b9b6eee0bbb44f |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-12-11T09:01:11Z |
| publishDate | 2018-06-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-9e8f1c7bfb1c492e91b9b6eee0bbb44f2022-12-22T01:13:46ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242018-06-0161010.1590/1678-4324-2016160613S1516-89132018000100400SSR-Marker assisted evaluation of Genetic Diversity in Mungbean ( Vigna radiata (L.) Wilcezk) genotypesGunnjeet KaurArunabh JoshiDevendra JainABSTRACT Mungbean (Vigna radiata (L.) Wilczek) also known as green gram is an important source of protein in the category of food legumes. In the present study, SSR marker is used to analyze the genetic diversity amongst 23 genotypes of mungbean. Out of a total of 10 primers used for SSR analysis revealed generation of 15 alleles. The number of alleles per locus ranged from one (CEDG006, CEDG010, CEDG050, CEDG088, CEDG092 and CEDG232) to three (CEDG 214), with an average of 1.5 allele per primer. The index for expected heterozygosity was 0.29 ranging from 0.15 to 0.49 revealed a deficit in heterozygosity. The size of amplification products varied in case of each primer and the range was found to be 100 bp to 190 bp. 13 out of 15 alleles were found polymorphic. The average PIC value of SSR marker was found to be 0.205. The value of Jaccard’s similarity coefficient had ranged from 0.28-1.00 with an average value of 0.64. The dendrogram constructed on SSR molecular marker data through UPGMA method and PCA using average linkage, had enabled grouping of the genotypes into three main clusters. Clustering pattern based on SSR marker data clearly indicated the narrow genetic base of mungbean genotypes that emphasizes the need to explore and exploit more number of germplasm from additional source to study genetic variation in mungbean for genetic improvement. The results indicated the marked usefulness of SSR in the assessment of genetic diversity in mungbean crop.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100400&lng=en&tlng=enGenetic diversityMungbeanSSR Marker assisted selection |
| spellingShingle | Gunnjeet Kaur Arunabh Joshi Devendra Jain SSR-Marker assisted evaluation of Genetic Diversity in Mungbean ( Vigna radiata (L.) Wilcezk) genotypes Brazilian Archives of Biology and Technology Genetic diversity Mungbean SSR Marker assisted selection |
| title | SSR-Marker assisted evaluation of Genetic Diversity in Mungbean ( Vigna radiata (L.) Wilcezk) genotypes |
| title_full | SSR-Marker assisted evaluation of Genetic Diversity in Mungbean ( Vigna radiata (L.) Wilcezk) genotypes |
| title_fullStr | SSR-Marker assisted evaluation of Genetic Diversity in Mungbean ( Vigna radiata (L.) Wilcezk) genotypes |
| title_full_unstemmed | SSR-Marker assisted evaluation of Genetic Diversity in Mungbean ( Vigna radiata (L.) Wilcezk) genotypes |
| title_short | SSR-Marker assisted evaluation of Genetic Diversity in Mungbean ( Vigna radiata (L.) Wilcezk) genotypes |
| title_sort | ssr marker assisted evaluation of genetic diversity in mungbean vigna radiata l wilcezk genotypes |
| topic | Genetic diversity Mungbean SSR Marker assisted selection |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100400&lng=en&tlng=en |
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