The effect of Green synthesized CuO Nanoparticles on callogenesis and regeneration of Oryza sativa L.
In this study, we have investigated the effect of copper oxide nanoparticles (CuO-NPs) on callogenesis and regeneration of Oryza sativa L (Super Basmati, Basmati 2000, Basmati 370 and Basmati 385). In this regard, CuO-NPs have been bio-synthesized via Azadirachta indica leaf extract. Scanning electr...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2016-08-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01330/full |
| _version_ | 1818335219539771392 |
|---|---|
| author | Sadaf Anwaar Qaisar Maqbool Qaisar Maqbool Nyla Jabeen Mudassar Nazar Fazal Abbas Fazal Abbas Bushra Nawaz Talib Hussain Syed Zaheer Hussain |
| author_facet | Sadaf Anwaar Qaisar Maqbool Qaisar Maqbool Nyla Jabeen Mudassar Nazar Fazal Abbas Fazal Abbas Bushra Nawaz Talib Hussain Syed Zaheer Hussain |
| author_sort | Sadaf Anwaar |
| collection | DOAJ |
| description | In this study, we have investigated the effect of copper oxide nanoparticles (CuO-NPs) on callogenesis and regeneration of Oryza sativa L (Super Basmati, Basmati 2000, Basmati 370 and Basmati 385). In this regard, CuO-NPs have been bio-synthesized via Azadirachta indica leaf extract. Scanning electron microscope (SEM) analysis depicts average particle size of 40±5 nm with highly homogenous and spherical morphology. X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) have been employed to confirm the phase purity of the synthesized NPs. It is found that CuO-NPs exhibit very promising results against callus induction. It is attributed to the fact that green synthesized CuO-NPs at optimum dosage possess very supportive effects on plant growth parameters. In contrast to callogenesis, differential regeneration pattern has been observed against all of the examined O. sativa L. indigenous verities. Overall observation concludes that CuO, being one of the essential plant nutrients, has greatly tailored the nutritive properties at nano-scale. |
| first_indexed | 2024-12-13T14:19:57Z |
| format | Article |
| id | doaj.art-9e9517e2cab24e47b510ff81a08003d3 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-13T14:19:57Z |
| publishDate | 2016-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-9e9517e2cab24e47b510ff81a08003d32022-12-21T23:42:06ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-08-01710.3389/fpls.2016.01330217278The effect of Green synthesized CuO Nanoparticles on callogenesis and regeneration of Oryza sativa L.Sadaf Anwaar0Qaisar Maqbool1Qaisar Maqbool2Nyla Jabeen3Mudassar Nazar4Fazal Abbas5Fazal Abbas6Bushra Nawaz7Talib Hussain8Syed Zaheer Hussain9International Islamic University Islamabad(IIUI), Islamabad, PakistanVirtual University of Pakistan, Lahore, PakistanInterdisciplinary Research Organization, University of Chakwal (UOC)International Islamic University Islamabad(IIUI), Islamabad, PakistanVirtual University of Pakistan, Lahore, PakistanInternational Islamic University Islamabad (IIUI), Islamabad, PakistanInterdisciplinary Research Organization, University of Chakwal (UOC)University of Engineering and Technology (UET), Sub-Campus Chakwal, PakistanNational Institute of Vacuum Science and Technology (NINVAST), Islamabad, PakistanQuaid-i-Azam University, Islamabad, PakistanIn this study, we have investigated the effect of copper oxide nanoparticles (CuO-NPs) on callogenesis and regeneration of Oryza sativa L (Super Basmati, Basmati 2000, Basmati 370 and Basmati 385). In this regard, CuO-NPs have been bio-synthesized via Azadirachta indica leaf extract. Scanning electron microscope (SEM) analysis depicts average particle size of 40±5 nm with highly homogenous and spherical morphology. X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) have been employed to confirm the phase purity of the synthesized NPs. It is found that CuO-NPs exhibit very promising results against callus induction. It is attributed to the fact that green synthesized CuO-NPs at optimum dosage possess very supportive effects on plant growth parameters. In contrast to callogenesis, differential regeneration pattern has been observed against all of the examined O. sativa L. indigenous verities. Overall observation concludes that CuO, being one of the essential plant nutrients, has greatly tailored the nutritive properties at nano-scale.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01330/fullRegenerationSEMFTIRcallus inductionXRDCopper oxide nanoparticles |
| spellingShingle | Sadaf Anwaar Qaisar Maqbool Qaisar Maqbool Nyla Jabeen Mudassar Nazar Fazal Abbas Fazal Abbas Bushra Nawaz Talib Hussain Syed Zaheer Hussain The effect of Green synthesized CuO Nanoparticles on callogenesis and regeneration of Oryza sativa L. Frontiers in Plant Science Regeneration SEM FTIR callus induction XRD Copper oxide nanoparticles |
| title | The effect of Green synthesized CuO Nanoparticles on callogenesis and regeneration of Oryza sativa L. |
| title_full | The effect of Green synthesized CuO Nanoparticles on callogenesis and regeneration of Oryza sativa L. |
| title_fullStr | The effect of Green synthesized CuO Nanoparticles on callogenesis and regeneration of Oryza sativa L. |
| title_full_unstemmed | The effect of Green synthesized CuO Nanoparticles on callogenesis and regeneration of Oryza sativa L. |
| title_short | The effect of Green synthesized CuO Nanoparticles on callogenesis and regeneration of Oryza sativa L. |
| title_sort | effect of green synthesized cuo nanoparticles on callogenesis and regeneration of oryza sativa l |
| topic | Regeneration SEM FTIR callus induction XRD Copper oxide nanoparticles |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01330/full |
| work_keys_str_mv | AT sadafanwaar theeffectofgreensynthesizedcuonanoparticlesoncallogenesisandregenerationoforyzasatival AT qaisarmaqbool theeffectofgreensynthesizedcuonanoparticlesoncallogenesisandregenerationoforyzasatival AT qaisarmaqbool theeffectofgreensynthesizedcuonanoparticlesoncallogenesisandregenerationoforyzasatival AT nylajabeen theeffectofgreensynthesizedcuonanoparticlesoncallogenesisandregenerationoforyzasatival AT mudassarnazar theeffectofgreensynthesizedcuonanoparticlesoncallogenesisandregenerationoforyzasatival AT fazalabbas theeffectofgreensynthesizedcuonanoparticlesoncallogenesisandregenerationoforyzasatival AT fazalabbas theeffectofgreensynthesizedcuonanoparticlesoncallogenesisandregenerationoforyzasatival AT bushranawaz theeffectofgreensynthesizedcuonanoparticlesoncallogenesisandregenerationoforyzasatival AT talibhussain theeffectofgreensynthesizedcuonanoparticlesoncallogenesisandregenerationoforyzasatival AT syedzaheerhussain theeffectofgreensynthesizedcuonanoparticlesoncallogenesisandregenerationoforyzasatival AT sadafanwaar effectofgreensynthesizedcuonanoparticlesoncallogenesisandregenerationoforyzasatival AT qaisarmaqbool effectofgreensynthesizedcuonanoparticlesoncallogenesisandregenerationoforyzasatival AT qaisarmaqbool effectofgreensynthesizedcuonanoparticlesoncallogenesisandregenerationoforyzasatival AT nylajabeen effectofgreensynthesizedcuonanoparticlesoncallogenesisandregenerationoforyzasatival AT mudassarnazar effectofgreensynthesizedcuonanoparticlesoncallogenesisandregenerationoforyzasatival AT fazalabbas effectofgreensynthesizedcuonanoparticlesoncallogenesisandregenerationoforyzasatival AT fazalabbas effectofgreensynthesizedcuonanoparticlesoncallogenesisandregenerationoforyzasatival AT bushranawaz effectofgreensynthesizedcuonanoparticlesoncallogenesisandregenerationoforyzasatival AT talibhussain effectofgreensynthesizedcuonanoparticlesoncallogenesisandregenerationoforyzasatival AT syedzaheerhussain effectofgreensynthesizedcuonanoparticlesoncallogenesisandregenerationoforyzasatival |