Investigation of osmotic shock effect on pulsed electric field treated S. cerevisiae yeast cells
Abstract Pulsed electric field (PEF) treatment is known to cause plasma membrane permeabilization of microorganisms, an effect known as electroporation. PEF treatment is very attractive since it can achieve permeabilization with or without lethal damage in accordance with desired results. This study...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-06-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-37719-4 |
| _version_ | 1797789792081018880 |
|---|---|
| author | Greta Gančytė Povilas Šimonis Arūnas Stirkė |
| author_facet | Greta Gančytė Povilas Šimonis Arūnas Stirkė |
| author_sort | Greta Gančytė |
| collection | DOAJ |
| description | Abstract Pulsed electric field (PEF) treatment is known to cause plasma membrane permeabilization of microorganisms, an effect known as electroporation. PEF treatment is very attractive since it can achieve permeabilization with or without lethal damage in accordance with desired results. This study aimed to expand the accomplishment of electroporation outcomes by applying sudden post-PEF osmotic composition change of the media. Changes in yeast cells’ viability, size and plasma membrane regeneration rate were evaluated. However, we still have questions about the intracellular biochemical processes responsible for plasma membrane recovery after electroporation. Our suggested candidate is the high osmolarity glycerol (HOG) kinase pathway. The HOG pathway in Saccharomyces cerevisiae yeasts is responsible for volume recovery after dangerous shape modifications and intracellular water disbalance caused by environmental osmotic pressure changes. Thus, we evaluated the HOG pathway inactivation effect on S. cerevisiae’s reaction to PEF treatment. Results showed that Hog1 deficient S. cerevisiae cells were considerably more sensitive to electric field treatment, confirming a link between the HOG pathway and S. cerevisiae recovery process after electroporation. By suddenly changing the osmolarity of the media after PEF we influenced the cells’ plasma membrane recovery rate, severity of permeabilization and survivability of yeast cells. Studies of electroporation in combination with various treatments might improve electric field application range, efficiency, and optimization of the process. |
| first_indexed | 2024-03-13T01:55:41Z |
| format | Article |
| id | doaj.art-9e956591dac6483ca34894b3e36e2a8b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T01:55:41Z |
| publishDate | 2023-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9e956591dac6483ca34894b3e36e2a8b2023-07-02T11:14:06ZengNature PortfolioScientific Reports2045-23222023-06-0113111110.1038/s41598-023-37719-4Investigation of osmotic shock effect on pulsed electric field treated S. cerevisiae yeast cellsGreta Gančytė0Povilas Šimonis1Arūnas Stirkė2Laboratory of Bioelectrics, Center for Physical Sciences and Technology, State Research InstituteLaboratory of Bioelectrics, Center for Physical Sciences and Technology, State Research InstituteLaboratory of Bioelectrics, Center for Physical Sciences and Technology, State Research InstituteAbstract Pulsed electric field (PEF) treatment is known to cause plasma membrane permeabilization of microorganisms, an effect known as electroporation. PEF treatment is very attractive since it can achieve permeabilization with or without lethal damage in accordance with desired results. This study aimed to expand the accomplishment of electroporation outcomes by applying sudden post-PEF osmotic composition change of the media. Changes in yeast cells’ viability, size and plasma membrane regeneration rate were evaluated. However, we still have questions about the intracellular biochemical processes responsible for plasma membrane recovery after electroporation. Our suggested candidate is the high osmolarity glycerol (HOG) kinase pathway. The HOG pathway in Saccharomyces cerevisiae yeasts is responsible for volume recovery after dangerous shape modifications and intracellular water disbalance caused by environmental osmotic pressure changes. Thus, we evaluated the HOG pathway inactivation effect on S. cerevisiae’s reaction to PEF treatment. Results showed that Hog1 deficient S. cerevisiae cells were considerably more sensitive to electric field treatment, confirming a link between the HOG pathway and S. cerevisiae recovery process after electroporation. By suddenly changing the osmolarity of the media after PEF we influenced the cells’ plasma membrane recovery rate, severity of permeabilization and survivability of yeast cells. Studies of electroporation in combination with various treatments might improve electric field application range, efficiency, and optimization of the process.https://doi.org/10.1038/s41598-023-37719-4 |
| spellingShingle | Greta Gančytė Povilas Šimonis Arūnas Stirkė Investigation of osmotic shock effect on pulsed electric field treated S. cerevisiae yeast cells Scientific Reports |
| title | Investigation of osmotic shock effect on pulsed electric field treated S. cerevisiae yeast cells |
| title_full | Investigation of osmotic shock effect on pulsed electric field treated S. cerevisiae yeast cells |
| title_fullStr | Investigation of osmotic shock effect on pulsed electric field treated S. cerevisiae yeast cells |
| title_full_unstemmed | Investigation of osmotic shock effect on pulsed electric field treated S. cerevisiae yeast cells |
| title_short | Investigation of osmotic shock effect on pulsed electric field treated S. cerevisiae yeast cells |
| title_sort | investigation of osmotic shock effect on pulsed electric field treated s cerevisiae yeast cells |
| url | https://doi.org/10.1038/s41598-023-37719-4 |
| work_keys_str_mv | AT gretagancyte investigationofosmoticshockeffectonpulsedelectricfieldtreatedscerevisiaeyeastcells AT povilassimonis investigationofosmoticshockeffectonpulsedelectricfieldtreatedscerevisiaeyeastcells AT arunasstirke investigationofosmoticshockeffectonpulsedelectricfieldtreatedscerevisiaeyeastcells |