Cytotoxicity, anti-inflammatory effect, and acute oral toxicity of a novel Attalea phalerata kernel oil-loaded nanocapsules
The kernel oil of the Attalea phalerata Mart. Ex Spreng (Acurí) is traditionally used in several Latin American countries to treat respiratory problems, inflammation, and fever. However, it cannot be found on the literature any attend to use this oil in pharmaceutical formulation. In this paper, it...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-05-01
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| Series: | Biomedicine & Pharmacotherapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332224001896 |
| _version_ | 1797203112851668992 |
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| author | Bianca Rodrigues Acácio Ariadna Lafourcade Prada Serafim Florentino Neto Giovana Bicudo Gomes Renata Trentin Perdomo Carlos Eduardo Domingues Nazario Eduardo Sobieski Neto Marco António Utrera Martines Danielle Ayr Tavares de Almeida Arquimedes Gasparotto Junior Jesus Rafael Rodriguez Amado |
| author_facet | Bianca Rodrigues Acácio Ariadna Lafourcade Prada Serafim Florentino Neto Giovana Bicudo Gomes Renata Trentin Perdomo Carlos Eduardo Domingues Nazario Eduardo Sobieski Neto Marco António Utrera Martines Danielle Ayr Tavares de Almeida Arquimedes Gasparotto Junior Jesus Rafael Rodriguez Amado |
| author_sort | Bianca Rodrigues Acácio |
| collection | DOAJ |
| description | The kernel oil of the Attalea phalerata Mart. Ex Spreng (Acurí) is traditionally used in several Latin American countries to treat respiratory problems, inflammation, and fever. However, it cannot be found on the literature any attend to use this oil in pharmaceutical formulation. In this paper, it was developed Acurí oil-loaded nanocapsules, and it was evaluated the cytotoxicity against cancer cells, the antinflammatory activity and the oral acute toxicity in rats. Acurí oil contains lauric acid as the predominant saturated fatty acid (433.26 mg/g) and oleic acid as the main unsaturated fatty acid (180.06 mg/g). The Acurí oil-loaded nanocapsules showed a size of 237 nm, a polydispersity index of 0.260, and a high ζ-potential of −78.75 mV. It was obtained an encapsulation efficiency of 88.77%, and the nanocapsules remain stable on the shelf for 180 days. The nanocapsules showed a rapid release profile (98.25% in 40 minutes). Nanocapsules at a dose of 10 mg/kg exhibit an anti-inflammatory effect similar to indomethacin at the same dose. The nanocapsules showed excellent antiproliferative effect and selectivity index against prostate tumor cells (IC50 2.09 µg/mL, SI=119.61) and kidney tumor cells (IC50 3.03 µg/mL, SI=82.50). Both Acurí oil and Acurí oil-loaded nanocapsules are nontoxic at a dose of 2000 mg/kg. Additionally, they reduce serum triglyceride and total cholesterol levels in rat and could find application in nutraceutical formulations. The Acurí oil-loaded nanocapsules emerge as a promising candidate for new antitumor therapies. |
| first_indexed | 2024-04-24T08:14:10Z |
| format | Article |
| id | doaj.art-9e970f676edb4af09fc6f8a0fa45000d |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-04-24T08:14:10Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-9e970f676edb4af09fc6f8a0fa45000d2024-04-17T04:48:30ZengElsevierBiomedicine & Pharmacotherapy0753-33222024-05-01174116308Cytotoxicity, anti-inflammatory effect, and acute oral toxicity of a novel Attalea phalerata kernel oil-loaded nanocapsulesBianca Rodrigues Acácio0Ariadna Lafourcade Prada1Serafim Florentino Neto2Giovana Bicudo Gomes3Renata Trentin Perdomo4Carlos Eduardo Domingues Nazario5Eduardo Sobieski Neto6Marco António Utrera Martines7Danielle Ayr Tavares de Almeida8Arquimedes Gasparotto Junior9Jesus Rafael Rodriguez Amado10Postgraduate Program in Pharmaceutical Sciences, Faculty of Pharmacy, Food, and Nutrition, Federal University of Mato Grosso do Sul, BrazilPostgraduate Program in Biotechnology, Faculty of Pharmacy, Food, and Nutrition, Federal University of Mato Grosso do Sul, BrazilLaboratory of Innovation in Pharmaceutical Technology, Federal University of Amazonas, Manaus, AM, BrazilPostgraduate Program in Pharmaceutical Sciences, Faculty of Pharmacy, Food, and Nutrition, Federal University of Mato Grosso do Sul, BrazilPostgraduate Program in Pharmaceutical Sciences, Faculty of Pharmacy, Food, and Nutrition, Federal University of Mato Grosso do Sul, BrazilPostgraduate Program in Biotechnology, Institute of Chemistry, Federal University of Mato Grosso do Sul, BrazilPostgraduate Program in Biotechnology, Institute of Chemistry, Federal University of Mato Grosso do Sul, BrazilPostgraduate Program in Biotechnology, Institute of Chemistry, Federal University of Mato Grosso do Sul, BrazilPostgraduate Program in Health Sciences. Faculty of Health Sciences, Federal University of Grande Dourados, Dourados, MS, BrazilPostgraduate Program in Health Sciences. Faculty of Health Sciences, Federal University of Grande Dourados, Dourados, MS, BrazilPostgraduate Program in Health Sciences. Faculty of Health Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil; Correspondence to: Postgraduate Program in Health Sciences. Faculty of Health Sciences, Federal University of Grande Dourados, RodovíaItahum, Km 12 - Cidade Universitária, Dourados, MS CEP 79804970, Brazil.The kernel oil of the Attalea phalerata Mart. Ex Spreng (Acurí) is traditionally used in several Latin American countries to treat respiratory problems, inflammation, and fever. However, it cannot be found on the literature any attend to use this oil in pharmaceutical formulation. In this paper, it was developed Acurí oil-loaded nanocapsules, and it was evaluated the cytotoxicity against cancer cells, the antinflammatory activity and the oral acute toxicity in rats. Acurí oil contains lauric acid as the predominant saturated fatty acid (433.26 mg/g) and oleic acid as the main unsaturated fatty acid (180.06 mg/g). The Acurí oil-loaded nanocapsules showed a size of 237 nm, a polydispersity index of 0.260, and a high ζ-potential of −78.75 mV. It was obtained an encapsulation efficiency of 88.77%, and the nanocapsules remain stable on the shelf for 180 days. The nanocapsules showed a rapid release profile (98.25% in 40 minutes). Nanocapsules at a dose of 10 mg/kg exhibit an anti-inflammatory effect similar to indomethacin at the same dose. The nanocapsules showed excellent antiproliferative effect and selectivity index against prostate tumor cells (IC50 2.09 µg/mL, SI=119.61) and kidney tumor cells (IC50 3.03 µg/mL, SI=82.50). Both Acurí oil and Acurí oil-loaded nanocapsules are nontoxic at a dose of 2000 mg/kg. Additionally, they reduce serum triglyceride and total cholesterol levels in rat and could find application in nutraceutical formulations. The Acurí oil-loaded nanocapsules emerge as a promising candidate for new antitumor therapies.http://www.sciencedirect.com/science/article/pii/S0753332224001896Attalea phalerataNanocapsulesCytotoxicityCancerAnti-inflammatoryAcurí oil |
| spellingShingle | Bianca Rodrigues Acácio Ariadna Lafourcade Prada Serafim Florentino Neto Giovana Bicudo Gomes Renata Trentin Perdomo Carlos Eduardo Domingues Nazario Eduardo Sobieski Neto Marco António Utrera Martines Danielle Ayr Tavares de Almeida Arquimedes Gasparotto Junior Jesus Rafael Rodriguez Amado Cytotoxicity, anti-inflammatory effect, and acute oral toxicity of a novel Attalea phalerata kernel oil-loaded nanocapsules Biomedicine & Pharmacotherapy Attalea phalerata Nanocapsules Cytotoxicity Cancer Anti-inflammatory Acurí oil |
| title | Cytotoxicity, anti-inflammatory effect, and acute oral toxicity of a novel Attalea phalerata kernel oil-loaded nanocapsules |
| title_full | Cytotoxicity, anti-inflammatory effect, and acute oral toxicity of a novel Attalea phalerata kernel oil-loaded nanocapsules |
| title_fullStr | Cytotoxicity, anti-inflammatory effect, and acute oral toxicity of a novel Attalea phalerata kernel oil-loaded nanocapsules |
| title_full_unstemmed | Cytotoxicity, anti-inflammatory effect, and acute oral toxicity of a novel Attalea phalerata kernel oil-loaded nanocapsules |
| title_short | Cytotoxicity, anti-inflammatory effect, and acute oral toxicity of a novel Attalea phalerata kernel oil-loaded nanocapsules |
| title_sort | cytotoxicity anti inflammatory effect and acute oral toxicity of a novel attalea phalerata kernel oil loaded nanocapsules |
| topic | Attalea phalerata Nanocapsules Cytotoxicity Cancer Anti-inflammatory Acurí oil |
| url | http://www.sciencedirect.com/science/article/pii/S0753332224001896 |
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