Deep sequencing identification of miRNAs in pigeon ovaries illuminated with monochromatic light
Abstract Background The use of light of different wavelengths has grown popular in the poultry industry. An optimum wavelength is believed to improve pigeon egg production, but little is known about the role of microRNAs (miRNAs) in the effects of monochromatic light on ovarian pigeon function. Here...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-06-01
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| Series: | BMC Genomics |
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| Online Access: | http://link.springer.com/article/10.1186/s12864-018-4831-6 |
| _version_ | 1811264653144096768 |
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| author | Ying Wang Hai-ming Yang Wei Cao Yang-bai Li Zhi-yue Wang |
| author_facet | Ying Wang Hai-ming Yang Wei Cao Yang-bai Li Zhi-yue Wang |
| author_sort | Ying Wang |
| collection | DOAJ |
| description | Abstract Background The use of light of different wavelengths has grown popular in the poultry industry. An optimum wavelength is believed to improve pigeon egg production, but little is known about the role of microRNAs (miRNAs) in the effects of monochromatic light on ovarian pigeon function. Herein, we harvested ovaries from pigeons reared under monochromatic light of different wavelength and performed deep sequencing on various tissues using an Illumina Solexa high-throughput instrument. Results We obtained 66,148,548, 67,873,805, and 71,661,771 clean reads from ovaries of pigeons reared under red light (RL), blue light (BL), and white light (WL), respectively. We identified 1917 known miRNAs in nine libraries, of which 524 were novel. Three and five differentially expressed miRNAs were identified in BL vs. WL and RL vs. WL groups, respectively. Quantitative reverse transcription PCR was used to validate differentially expressed miRNAs (miR-200, miR-122, and miR-205b). In addition, 5824 target genes were annotated as differentially expressed miRNAs, most of which are involved in reproductive pathways including oestrogen signalling, cell cycle, and oocyte maturation. Notably, ovarian miR-205b expression was significantly negatively correlated with its target 11β-hydroxysteroid dehydrogenase type 1 (HSD11B1). Conclusions miRNA–mRNA network analysis suggests that miR-205b targeting of HSD11B1 plays a key role in the effects of monochromatic light on pigeon egg production. These findings indicate that monochromatic light shortens the oviposition interval of pigeons, which may be useful for egg production and pigeon breeding. |
| first_indexed | 2024-04-12T20:08:16Z |
| format | Article |
| id | doaj.art-9e98b54baab74264be570f0b0972f965 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-12T20:08:16Z |
| publishDate | 2018-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-9e98b54baab74264be570f0b0972f9652022-12-22T03:18:19ZengBMCBMC Genomics1471-21642018-06-011911910.1186/s12864-018-4831-6Deep sequencing identification of miRNAs in pigeon ovaries illuminated with monochromatic lightYing Wang0Hai-ming Yang1Wei Cao2Yang-bai Li3Zhi-yue Wang4College of Animal Science and Technology, Yangzhou UniversityCollege of Animal Science and Technology, Yangzhou UniversityCollege of Animal Science and Technology, Yangzhou UniversityCollege of Animal Science and Technology, Yangzhou UniversityCollege of Animal Science and Technology, Yangzhou UniversityAbstract Background The use of light of different wavelengths has grown popular in the poultry industry. An optimum wavelength is believed to improve pigeon egg production, but little is known about the role of microRNAs (miRNAs) in the effects of monochromatic light on ovarian pigeon function. Herein, we harvested ovaries from pigeons reared under monochromatic light of different wavelength and performed deep sequencing on various tissues using an Illumina Solexa high-throughput instrument. Results We obtained 66,148,548, 67,873,805, and 71,661,771 clean reads from ovaries of pigeons reared under red light (RL), blue light (BL), and white light (WL), respectively. We identified 1917 known miRNAs in nine libraries, of which 524 were novel. Three and five differentially expressed miRNAs were identified in BL vs. WL and RL vs. WL groups, respectively. Quantitative reverse transcription PCR was used to validate differentially expressed miRNAs (miR-200, miR-122, and miR-205b). In addition, 5824 target genes were annotated as differentially expressed miRNAs, most of which are involved in reproductive pathways including oestrogen signalling, cell cycle, and oocyte maturation. Notably, ovarian miR-205b expression was significantly negatively correlated with its target 11β-hydroxysteroid dehydrogenase type 1 (HSD11B1). Conclusions miRNA–mRNA network analysis suggests that miR-205b targeting of HSD11B1 plays a key role in the effects of monochromatic light on pigeon egg production. These findings indicate that monochromatic light shortens the oviposition interval of pigeons, which may be useful for egg production and pigeon breeding.http://link.springer.com/article/10.1186/s12864-018-4831-6PigeonmicroRNAMonochromatic lightEgg production |
| spellingShingle | Ying Wang Hai-ming Yang Wei Cao Yang-bai Li Zhi-yue Wang Deep sequencing identification of miRNAs in pigeon ovaries illuminated with monochromatic light BMC Genomics Pigeon microRNA Monochromatic light Egg production |
| title | Deep sequencing identification of miRNAs in pigeon ovaries illuminated with monochromatic light |
| title_full | Deep sequencing identification of miRNAs in pigeon ovaries illuminated with monochromatic light |
| title_fullStr | Deep sequencing identification of miRNAs in pigeon ovaries illuminated with monochromatic light |
| title_full_unstemmed | Deep sequencing identification of miRNAs in pigeon ovaries illuminated with monochromatic light |
| title_short | Deep sequencing identification of miRNAs in pigeon ovaries illuminated with monochromatic light |
| title_sort | deep sequencing identification of mirnas in pigeon ovaries illuminated with monochromatic light |
| topic | Pigeon microRNA Monochromatic light Egg production |
| url | http://link.springer.com/article/10.1186/s12864-018-4831-6 |
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