Effects of vardenafil on the kidney of Wistar rats submitted to acute ischemia and reperfusion
PURPOSE: To investigate the effect of vardenafil in kidney of rats submitted to acute ischemia and reperfusion.METHODS:Twenty-eight rats were randomly distributed into two groups. Right nephrectomy was performed and the vardenafil group received vardenafil solution (at a concentration of 1 mg/ml in...
| Główni autorzy: | , , , , |
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| Format: | Artykuł |
| Język: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2015-05-01
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| Seria: | Acta Cirúrgica Brasileira |
| Hasła przedmiotowe: | |
| Dostęp online: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502015000500339&tlng=en |
| _version_ | 1828428835725508608 |
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| author | Renato Chavasco de Sousa Antonio Augusto Moreira Neto Vera Luíza Capelozzi Alexandre Muxfeldt Ab'Saber Olavo Ribeiro Rodrigues |
| author_facet | Renato Chavasco de Sousa Antonio Augusto Moreira Neto Vera Luíza Capelozzi Alexandre Muxfeldt Ab'Saber Olavo Ribeiro Rodrigues |
| author_sort | Renato Chavasco de Sousa |
| collection | DOAJ |
| description | PURPOSE: To investigate the effect of vardenafil in kidney of rats submitted to acute ischemia and reperfusion.METHODS:Twenty-eight rats were randomly distributed into two groups. Right nephrectomy was performed and the vardenafil group received vardenafil solution (at a concentration of 1 mg/ml in 10 mg/kg) while the control group received 0.9% saline solution (SS) one hour prior to the ligature of the left renal pedicle. After one hour of ischemia, animals were submitted to twenty-four hours of reperfusion, followed by left nephrectomy. The kidney's histological parameters evaluated on the study included vacuolar degeneration and tubular necrosis. Apoptosis was assessed by immunohistochemistry for cleaved caspase-3 using the point-counting and digital methods (Cytophotometry). Also, a biochemical analysis for creatinine was conducted.RESULTS: There were statistically significant differences between groups only with regards to the vacuolar degeneration parameter and to the cleaved caspase-3 digital method.CONCLUSION: Vardenafil showed a protective effect on the kidney of rats subjected to acute ischemia and reperfusion in this model |
| first_indexed | 2024-12-10T17:23:50Z |
| format | Article |
| id | doaj.art-9e99a8b801b846c99dcad0f07e04b32c |
| institution | Directory Open Access Journal |
| issn | 0102-8650 |
| language | English |
| last_indexed | 2024-12-10T17:23:50Z |
| publishDate | 2015-05-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-9e99a8b801b846c99dcad0f07e04b32c2022-12-22T01:39:54ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira0102-86502015-05-0130533934410.1590/S0102-865020150050000005Effects of vardenafil on the kidney of Wistar rats submitted to acute ischemia and reperfusionRenato Chavasco de SousaAntonio Augusto Moreira NetoVera Luíza CapelozziAlexandre Muxfeldt Ab'SaberOlavo Ribeiro RodriguesPURPOSE: To investigate the effect of vardenafil in kidney of rats submitted to acute ischemia and reperfusion.METHODS:Twenty-eight rats were randomly distributed into two groups. Right nephrectomy was performed and the vardenafil group received vardenafil solution (at a concentration of 1 mg/ml in 10 mg/kg) while the control group received 0.9% saline solution (SS) one hour prior to the ligature of the left renal pedicle. After one hour of ischemia, animals were submitted to twenty-four hours of reperfusion, followed by left nephrectomy. The kidney's histological parameters evaluated on the study included vacuolar degeneration and tubular necrosis. Apoptosis was assessed by immunohistochemistry for cleaved caspase-3 using the point-counting and digital methods (Cytophotometry). Also, a biochemical analysis for creatinine was conducted.RESULTS: There were statistically significant differences between groups only with regards to the vacuolar degeneration parameter and to the cleaved caspase-3 digital method.CONCLUSION: Vardenafil showed a protective effect on the kidney of rats subjected to acute ischemia and reperfusion in this modelhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502015000500339&tlng=enIschemiaReperfusionImmunohistochemistryHistologyRats |
| spellingShingle | Renato Chavasco de Sousa Antonio Augusto Moreira Neto Vera Luíza Capelozzi Alexandre Muxfeldt Ab'Saber Olavo Ribeiro Rodrigues Effects of vardenafil on the kidney of Wistar rats submitted to acute ischemia and reperfusion Acta Cirúrgica Brasileira Ischemia Reperfusion Immunohistochemistry Histology Rats |
| title | Effects of vardenafil on the kidney of Wistar rats submitted to acute ischemia and reperfusion |
| title_full | Effects of vardenafil on the kidney of Wistar rats submitted to acute ischemia and reperfusion |
| title_fullStr | Effects of vardenafil on the kidney of Wistar rats submitted to acute ischemia and reperfusion |
| title_full_unstemmed | Effects of vardenafil on the kidney of Wistar rats submitted to acute ischemia and reperfusion |
| title_short | Effects of vardenafil on the kidney of Wistar rats submitted to acute ischemia and reperfusion |
| title_sort | effects of vardenafil on the kidney of wistar rats submitted to acute ischemia and reperfusion |
| topic | Ischemia Reperfusion Immunohistochemistry Histology Rats |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502015000500339&tlng=en |
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