Anti-Inflammatory Effects of Camellia fascicularis Polyphenols via Attenuation of NF-κB and MAPK Pathways in LPS-Induced THP-1 Macrophages
Miaozi Gao,1,2 Xiaowei Peng,3 Junrong Tang,1 Jia Deng,1 Fang Wang,1 Yingjun Zhang,4 Ping Zhao,1,2 Huan Kan,1,3 Yun Liu1,3 1Key Laboratory of Forest Resources Conservation and Utilization in the Southwest Mountains of China Ministry of Education, Southwest Forestry University, Kunming, People’s Repub...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2022-02-01
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| Series: | Journal of Inflammation Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/anti-inflammatory-effects-of-camellia-fascicularis-polyphenols-via-att-peer-reviewed-fulltext-article-JIR |
| _version_ | 1818284922867023872 |
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| author | Gao M Peng X Tang J Deng J Wang F Zhang Y Zhao P Kan H Liu Y |
| author_facet | Gao M Peng X Tang J Deng J Wang F Zhang Y Zhao P Kan H Liu Y |
| author_sort | Gao M |
| collection | DOAJ |
| description | Miaozi Gao,1,2 Xiaowei Peng,3 Junrong Tang,1 Jia Deng,1 Fang Wang,1 Yingjun Zhang,4 Ping Zhao,1,2 Huan Kan,1,3 Yun Liu1,3 1Key Laboratory of Forest Resources Conservation and Utilization in the Southwest Mountains of China Ministry of Education, Southwest Forestry University, Kunming, People’s Republic of China; 2Key Laboratory of State Forestry and Grassland Administration on Highly-efficient Utilization of Forestry Biomass Resources in Southwest China, Southwest Forestry University, Kunming, People’s Republic of China; 3School of Life Science, Southwest Forestry University, Kunming, People’s Republic of China; 4State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, People’s Republic of ChinaCorrespondence: Ping Zhao; Yun LiuKey Laboratory of Forest Resources Conservation and Utilization in the Southwest Mountains of China Ministry of Education, Southwest Forestry University, #300, Bailong Road, Kunming, 650224, People’s Republic of China, Email hypzhao@yahoo.com; liuyun@swfu.edu.cnPurpose: Plant polyphenols possess beneficial functions against various diseases. This study aimed to identify phenolic ingredients in Camellia fascicularis (C. fascicularis) and investigate its possible underlying anti-inflammatory mechanism in lipopolysaccharide (LPS)-induced human monocytes (THP-1) macrophages.Methods: C. fascicularis polyphenols (CFP) were characterized by ultra-performance liquid chromatography (UPLC) combined with quadrupole-time-of-flight mass/mass spectrometry (Q-TOF-MS/MS). The THP-1 cells were differentiated into macrophages under the stimulation of phorbol 12-myristate 13-acetate (PMA) and then treated with LPS to build a cellular inflammation model. The cell viability was detected by CCK-8 assay. The levels of reactive oxygen species (ROS) were assessed by flow cytometry. The secretion and expression of inflammatory cytokines were tested by enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (RT-PCR). In addition, the nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways were analyzed by Western blotting.Results: Twelve phenolic constituents including (–)-epicatechin, casuariin, agastachoside, etc. in CFP were identified. The CCK-8 assay showed that CFP exhibited no significant cytotoxicity between 100 and 300 μg/mL. After treated with CFP, the release of ROS was significantly suppressed. CFP inhibited inflammation in macrophages by attenuating the polarization of LPS-induced THP-1 macrophages, down-regulating the expression of the pro-inflammatory cytokines IL-6, IL-1β and TNF-α, and up-regulating the expression of the anti-inflammatory cytokine IL-10. Western blotting experiments manifested that CFP could markedly inhibit the phosphorylation of p65, ERK and JNK, thereby suppressing the activation of NF-κB and MAPK signaling pathways.Conclusion: These findings indicated that CFP exerted anti-inflammatory activity by inhibiting the activation NF-κB and MAPK pathways which may induce the secretion of pro-inflammatory cytokines. This study offers a reference for C. fascicularis as the source of developing natural, safe anti-inflammatory agents in the future.Keywords: Camellia fascicularis, polyphenols, anti-inflammatory activity, NF-κB, MAPK |
| first_indexed | 2024-12-13T01:00:30Z |
| format | Article |
| id | doaj.art-9e9c4298c46c4c7cb022776ff80823a6 |
| institution | Directory Open Access Journal |
| issn | 1178-7031 |
| language | English |
| last_indexed | 2024-12-13T01:00:30Z |
| publishDate | 2022-02-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Inflammation Research |
| spelling | doaj.art-9e9c4298c46c4c7cb022776ff80823a62022-12-22T00:04:41ZengDove Medical PressJournal of Inflammation Research1178-70312022-02-01Volume 1585186472777Anti-Inflammatory Effects of Camellia fascicularis Polyphenols via Attenuation of NF-κB and MAPK Pathways in LPS-Induced THP-1 MacrophagesGao MPeng XTang JDeng JWang FZhang YZhao PKan HLiu YMiaozi Gao,1,2 Xiaowei Peng,3 Junrong Tang,1 Jia Deng,1 Fang Wang,1 Yingjun Zhang,4 Ping Zhao,1,2 Huan Kan,1,3 Yun Liu1,3 1Key Laboratory of Forest Resources Conservation and Utilization in the Southwest Mountains of China Ministry of Education, Southwest Forestry University, Kunming, People’s Republic of China; 2Key Laboratory of State Forestry and Grassland Administration on Highly-efficient Utilization of Forestry Biomass Resources in Southwest China, Southwest Forestry University, Kunming, People’s Republic of China; 3School of Life Science, Southwest Forestry University, Kunming, People’s Republic of China; 4State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, People’s Republic of ChinaCorrespondence: Ping Zhao; Yun LiuKey Laboratory of Forest Resources Conservation and Utilization in the Southwest Mountains of China Ministry of Education, Southwest Forestry University, #300, Bailong Road, Kunming, 650224, People’s Republic of China, Email hypzhao@yahoo.com; liuyun@swfu.edu.cnPurpose: Plant polyphenols possess beneficial functions against various diseases. This study aimed to identify phenolic ingredients in Camellia fascicularis (C. fascicularis) and investigate its possible underlying anti-inflammatory mechanism in lipopolysaccharide (LPS)-induced human monocytes (THP-1) macrophages.Methods: C. fascicularis polyphenols (CFP) were characterized by ultra-performance liquid chromatography (UPLC) combined with quadrupole-time-of-flight mass/mass spectrometry (Q-TOF-MS/MS). The THP-1 cells were differentiated into macrophages under the stimulation of phorbol 12-myristate 13-acetate (PMA) and then treated with LPS to build a cellular inflammation model. The cell viability was detected by CCK-8 assay. The levels of reactive oxygen species (ROS) were assessed by flow cytometry. The secretion and expression of inflammatory cytokines were tested by enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (RT-PCR). In addition, the nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways were analyzed by Western blotting.Results: Twelve phenolic constituents including (–)-epicatechin, casuariin, agastachoside, etc. in CFP were identified. The CCK-8 assay showed that CFP exhibited no significant cytotoxicity between 100 and 300 μg/mL. After treated with CFP, the release of ROS was significantly suppressed. CFP inhibited inflammation in macrophages by attenuating the polarization of LPS-induced THP-1 macrophages, down-regulating the expression of the pro-inflammatory cytokines IL-6, IL-1β and TNF-α, and up-regulating the expression of the anti-inflammatory cytokine IL-10. Western blotting experiments manifested that CFP could markedly inhibit the phosphorylation of p65, ERK and JNK, thereby suppressing the activation of NF-κB and MAPK signaling pathways.Conclusion: These findings indicated that CFP exerted anti-inflammatory activity by inhibiting the activation NF-κB and MAPK pathways which may induce the secretion of pro-inflammatory cytokines. This study offers a reference for C. fascicularis as the source of developing natural, safe anti-inflammatory agents in the future.Keywords: Camellia fascicularis, polyphenols, anti-inflammatory activity, NF-κB, MAPKhttps://www.dovepress.com/anti-inflammatory-effects-of-camellia-fascicularis-polyphenols-via-att-peer-reviewed-fulltext-article-JIRcamellia fascicularispolyphenolsanti-inflammatory activitynf-κbmapk |
| spellingShingle | Gao M Peng X Tang J Deng J Wang F Zhang Y Zhao P Kan H Liu Y Anti-Inflammatory Effects of Camellia fascicularis Polyphenols via Attenuation of NF-κB and MAPK Pathways in LPS-Induced THP-1 Macrophages Journal of Inflammation Research camellia fascicularis polyphenols anti-inflammatory activity nf-κb mapk |
| title | Anti-Inflammatory Effects of Camellia fascicularis Polyphenols via Attenuation of NF-κB and MAPK Pathways in LPS-Induced THP-1 Macrophages |
| title_full | Anti-Inflammatory Effects of Camellia fascicularis Polyphenols via Attenuation of NF-κB and MAPK Pathways in LPS-Induced THP-1 Macrophages |
| title_fullStr | Anti-Inflammatory Effects of Camellia fascicularis Polyphenols via Attenuation of NF-κB and MAPK Pathways in LPS-Induced THP-1 Macrophages |
| title_full_unstemmed | Anti-Inflammatory Effects of Camellia fascicularis Polyphenols via Attenuation of NF-κB and MAPK Pathways in LPS-Induced THP-1 Macrophages |
| title_short | Anti-Inflammatory Effects of Camellia fascicularis Polyphenols via Attenuation of NF-κB and MAPK Pathways in LPS-Induced THP-1 Macrophages |
| title_sort | anti inflammatory effects of camellia fascicularis polyphenols via attenuation of nf kappa b and mapk pathways in lps induced thp 1 macrophages |
| topic | camellia fascicularis polyphenols anti-inflammatory activity nf-κb mapk |
| url | https://www.dovepress.com/anti-inflammatory-effects-of-camellia-fascicularis-polyphenols-via-att-peer-reviewed-fulltext-article-JIR |
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