Yeast Surface Display System: Strategies for Improvement and Biotechnological Applications
Yeast surface display (YSD) is a “whole-cell” platform used for the heterologous expression of proteins immobilized on the yeast’s cell surface. YSD combines the advantages eukaryotic systems offer such as post-translational modifications, correct folding and glycosylation of proteins, with ease of...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2021.794742/full |
| _version_ | 1830373832623390720 |
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| author | Karla V. Teymennet-Ramírez Fernando Martínez-Morales María R. Trejo-Hernández |
| author_facet | Karla V. Teymennet-Ramírez Fernando Martínez-Morales María R. Trejo-Hernández |
| author_sort | Karla V. Teymennet-Ramírez |
| collection | DOAJ |
| description | Yeast surface display (YSD) is a “whole-cell” platform used for the heterologous expression of proteins immobilized on the yeast’s cell surface. YSD combines the advantages eukaryotic systems offer such as post-translational modifications, correct folding and glycosylation of proteins, with ease of cell culturing and genetic manipulation, and allows of protein immobilization and recovery. Additionally, proteins displayed on the surface of yeast cells may show enhanced stability against changes in temperature, pH, organic solvents, and proteases. This platform has been used to study protein-protein interactions, antibody design and protein engineering. Other applications for YSD include library screening, whole-proteome studies, bioremediation, vaccine and antibiotics development, production of biosensors, ethanol production and biocatalysis. YSD is a promising technology that is not yet optimized for biotechnological applications. This mini review is focused on recent strategies to improve the efficiency and selection of displayed proteins. YSD is presented as a cutting-edge technology for the vectorial expression of proteins and peptides. Finally, recent biotechnological applications are summarized. The different approaches described herein could allow for a better strategy cascade for increasing protein/peptide interaction and production. |
| first_indexed | 2024-12-20T07:25:41Z |
| format | Article |
| id | doaj.art-9e9e4933a7904b91acfa87f790e06cb7 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-20T07:25:41Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-9e9e4933a7904b91acfa87f790e06cb72022-12-21T19:48:32ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-01-01910.3389/fbioe.2021.794742794742Yeast Surface Display System: Strategies for Improvement and Biotechnological ApplicationsKarla V. Teymennet-RamírezFernando Martínez-MoralesMaría R. Trejo-HernándezYeast surface display (YSD) is a “whole-cell” platform used for the heterologous expression of proteins immobilized on the yeast’s cell surface. YSD combines the advantages eukaryotic systems offer such as post-translational modifications, correct folding and glycosylation of proteins, with ease of cell culturing and genetic manipulation, and allows of protein immobilization and recovery. Additionally, proteins displayed on the surface of yeast cells may show enhanced stability against changes in temperature, pH, organic solvents, and proteases. This platform has been used to study protein-protein interactions, antibody design and protein engineering. Other applications for YSD include library screening, whole-proteome studies, bioremediation, vaccine and antibiotics development, production of biosensors, ethanol production and biocatalysis. YSD is a promising technology that is not yet optimized for biotechnological applications. This mini review is focused on recent strategies to improve the efficiency and selection of displayed proteins. YSD is presented as a cutting-edge technology for the vectorial expression of proteins and peptides. Finally, recent biotechnological applications are summarized. The different approaches described herein could allow for a better strategy cascade for increasing protein/peptide interaction and production.https://www.frontiersin.org/articles/10.3389/fbioe.2021.794742/fullcell surface displayyeastanchormicrobial engineeringwhole cell biocatalyst |
| spellingShingle | Karla V. Teymennet-Ramírez Fernando Martínez-Morales María R. Trejo-Hernández Yeast Surface Display System: Strategies for Improvement and Biotechnological Applications Frontiers in Bioengineering and Biotechnology cell surface display yeast anchor microbial engineering whole cell biocatalyst |
| title | Yeast Surface Display System: Strategies for Improvement and Biotechnological Applications |
| title_full | Yeast Surface Display System: Strategies for Improvement and Biotechnological Applications |
| title_fullStr | Yeast Surface Display System: Strategies for Improvement and Biotechnological Applications |
| title_full_unstemmed | Yeast Surface Display System: Strategies for Improvement and Biotechnological Applications |
| title_short | Yeast Surface Display System: Strategies for Improvement and Biotechnological Applications |
| title_sort | yeast surface display system strategies for improvement and biotechnological applications |
| topic | cell surface display yeast anchor microbial engineering whole cell biocatalyst |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2021.794742/full |
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