Gut microbiota analysis in colorectal diseased patients in Menoufia University Hospitals, Egypt.
Background: Gut microbiota is a diverse group of bacteria living in digestive tract of human. Imbalance of this community (dysbiosis) was linked to several gastrointestinal diseases. Our objective was to assess the alterations in gut microbiota among patients with colorectal disorders. Methodology:...
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Zagazig University, Faculty of Medicine
2024-02-01
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| Series: | Microbes and Infectious Diseases |
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| Online Access: | https://mid.journals.ekb.eg/article_337252_b532d612fb5e59e99631cf9669388172.pdf |
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| author | Shymaa Elaskary Ayman Elgamal Hanem Badawy Marwa khalil Doaa Elgendy Heba Elhagary Amal Dawoud |
| author_facet | Shymaa Elaskary Ayman Elgamal Hanem Badawy Marwa khalil Doaa Elgendy Heba Elhagary Amal Dawoud |
| author_sort | Shymaa Elaskary |
| collection | DOAJ |
| description | Background: Gut microbiota is a diverse group of bacteria living in digestive tract of human. Imbalance of this community (dysbiosis) was linked to several gastrointestinal diseases. Our objective was to assess the alterations in gut microbiota among patients with colorectal disorders. Methodology: This study enrolled 70 patients with colorectal diseases and 30 controls. All participants were subjected to total colonoscopy and biopsy taking for histopathology investigation. Stool samples were collected, homogenized and divided to four portions for aerobic, anaerobic culture and 16S rRNA PCR based sequencing analysis. Results: This study included 30 patients with ulcerative colitis (UC), 20 patients with colorectal adenoma (CRA) and 20 patients with colorectal carcinoma (CRC). Regarding microbiota analysis in controls, Firmicutes, Actinobacteria, Proteobacteria and Bacteroidetes represented 72.7%, 15.1%, 9.1% and 3.0% respectively. None of the potential pathogens H. pylori and Pseudomonas spp. were isolated. For UC patients, Firmicutes, Proteobacteria and Actinobacteria represented 51.4%, 32.4% and 14.3% respectively. None of Bifidobacterium spp. was isolated from UC patients. For CRA and CRC patients, Proteobacteria was the most frequently isolated (38.7%, 56.7%) followed by Firmicutes (29.0%, 17.8%) and then the Bacteroidetes (20.9%, 13.4%) respectively. Isolated H. pylori and Pseudomonas spp. represented (9.6% &16.4%) and (8.1% & 14.9%) from CRA and CRC patients respectively. The totally isolated Firmicutes in controls, UC, CRA and CRC patients were 24, 3.6, 1.4 and 2 times the isolated Bacteroidetes respectively. Conclusion: Gut microbiota differs between patients and controls. Future studies can assess modifying gut microbiota in high-risk CRC patients as a preventative intervention. |
| first_indexed | 2024-03-08T03:32:28Z |
| format | Article |
| id | doaj.art-9ea093b7c0f847ed9c4ffc7553fb0514 |
| institution | Directory Open Access Journal |
| issn | 2682-4132 2682-4140 |
| language | English |
| last_indexed | 2024-03-08T03:32:28Z |
| publishDate | 2024-02-01 |
| publisher | Zagazig University, Faculty of Medicine |
| record_format | Article |
| series | Microbes and Infectious Diseases |
| spelling | doaj.art-9ea093b7c0f847ed9c4ffc7553fb05142024-02-10T21:57:30ZengZagazig University, Faculty of MedicineMicrobes and Infectious Diseases2682-41322682-41402024-02-015123024610.21608/mid.2024.257269.1726337252Gut microbiota analysis in colorectal diseased patients in Menoufia University Hospitals, Egypt.Shymaa Elaskary0Ayman Elgamal1Hanem Badawy2Marwa khalil3Doaa Elgendy4Heba Elhagary5Amal Dawoud6Medical Microbiology and Immunology Department, Faculty of Medicine, Menoufia University, EgyptTropical Medicine Department, Faculty of Medicine, Menoufia University, EgyptClinical Pathology Department, Faculty of Medicine, Menoufia University, EgyptMedical Biochemistry and Molecular Biology department, Faculty of Medicine, Menoufia University, EgyptInternal Medicine Department, Faculty of Medicine, Menoufia University, EgyptTropical Medicine Department, Faculty of Medicine, Menoufia University, EgyptMedical Microbiology and Immunology Department, Faculty of Medicine, Menoufia University, EgyptBackground: Gut microbiota is a diverse group of bacteria living in digestive tract of human. Imbalance of this community (dysbiosis) was linked to several gastrointestinal diseases. Our objective was to assess the alterations in gut microbiota among patients with colorectal disorders. Methodology: This study enrolled 70 patients with colorectal diseases and 30 controls. All participants were subjected to total colonoscopy and biopsy taking for histopathology investigation. Stool samples were collected, homogenized and divided to four portions for aerobic, anaerobic culture and 16S rRNA PCR based sequencing analysis. Results: This study included 30 patients with ulcerative colitis (UC), 20 patients with colorectal adenoma (CRA) and 20 patients with colorectal carcinoma (CRC). Regarding microbiota analysis in controls, Firmicutes, Actinobacteria, Proteobacteria and Bacteroidetes represented 72.7%, 15.1%, 9.1% and 3.0% respectively. None of the potential pathogens H. pylori and Pseudomonas spp. were isolated. For UC patients, Firmicutes, Proteobacteria and Actinobacteria represented 51.4%, 32.4% and 14.3% respectively. None of Bifidobacterium spp. was isolated from UC patients. For CRA and CRC patients, Proteobacteria was the most frequently isolated (38.7%, 56.7%) followed by Firmicutes (29.0%, 17.8%) and then the Bacteroidetes (20.9%, 13.4%) respectively. Isolated H. pylori and Pseudomonas spp. represented (9.6% &16.4%) and (8.1% & 14.9%) from CRA and CRC patients respectively. The totally isolated Firmicutes in controls, UC, CRA and CRC patients were 24, 3.6, 1.4 and 2 times the isolated Bacteroidetes respectively. Conclusion: Gut microbiota differs between patients and controls. Future studies can assess modifying gut microbiota in high-risk CRC patients as a preventative intervention.https://mid.journals.ekb.eg/article_337252_b532d612fb5e59e99631cf9669388172.pdfcolorectal disordersdysbiosismicrobiotasequencing |
| spellingShingle | Shymaa Elaskary Ayman Elgamal Hanem Badawy Marwa khalil Doaa Elgendy Heba Elhagary Amal Dawoud Gut microbiota analysis in colorectal diseased patients in Menoufia University Hospitals, Egypt. Microbes and Infectious Diseases colorectal disorders dysbiosis microbiota sequencing |
| title | Gut microbiota analysis in colorectal diseased patients in Menoufia University Hospitals, Egypt. |
| title_full | Gut microbiota analysis in colorectal diseased patients in Menoufia University Hospitals, Egypt. |
| title_fullStr | Gut microbiota analysis in colorectal diseased patients in Menoufia University Hospitals, Egypt. |
| title_full_unstemmed | Gut microbiota analysis in colorectal diseased patients in Menoufia University Hospitals, Egypt. |
| title_short | Gut microbiota analysis in colorectal diseased patients in Menoufia University Hospitals, Egypt. |
| title_sort | gut microbiota analysis in colorectal diseased patients in menoufia university hospitals egypt |
| topic | colorectal disorders dysbiosis microbiota sequencing |
| url | https://mid.journals.ekb.eg/article_337252_b532d612fb5e59e99631cf9669388172.pdf |
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