Lipid transfer particle mediates the delivery of diacylglycerol from lipophorin to fat body in larval Manduca sexta
This work analyzed the process of lipid storage in fat body of larval Manduca sexta, focusing on the role of lipid transfer particle (LTP). Incubation of fat bodies with [3H]diacylglycerol-labeled lipophorin resulted in a significant accumulation of diacylglycerol (DAG) and triacylglycerol (TAG) in...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2004-03-01
|
| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520318733 |
| _version_ | 1829513943650926592 |
|---|---|
| author | Lilian E. Canavoso Hwa Kyung Yun Zeina E. Jouni Michael A. Wells |
| author_facet | Lilian E. Canavoso Hwa Kyung Yun Zeina E. Jouni Michael A. Wells |
| author_sort | Lilian E. Canavoso |
| collection | DOAJ |
| description | This work analyzed the process of lipid storage in fat body of larval Manduca sexta, focusing on the role of lipid transfer particle (LTP). Incubation of fat bodies with [3H]diacylglycerol-labeled lipophorin resulted in a significant accumulation of diacylglycerol (DAG) and triacylglycerol (TAG) in the tissue. Transfer of DAG to fat body and its storage as TAG was significantly inhibited (60%) by preincubating the tissue with anti-LTP antibody. Lipid transfer was restored to control values by adding LTP to fat body. Incubation of fat body with dual-labeled DAG lipophorin or its treatment with ammonium chloride showed that neither a membrane-bound lipoprotein lipase nor lipophorin endocytosis is a relevant pathway to transfer or to storage lipids into fat body, respectively. Treatment of fat body with suramin caused a 50% inhibition in [3H]DAG transfer from lipophorin. Treatment of [3H]DAG-labeled fat body with lipase significantly reduced the amount of [3H]DAG associated with the tissue, suggesting that the lipid is still on the external surface of the membrane.Whether this lipid represents irreversibly adsorbed lipophorin or a DAG lipase-sensitive pool is unknown. Nevertheless, these results indicate that the main pathway for DAG transfer from lipophorin to fat body is via LTP and receptor-mediated processes. |
| first_indexed | 2024-12-16T13:09:25Z |
| format | Article |
| id | doaj.art-9ea18df498574f39b37c7d8e28977b72 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-16T13:09:25Z |
| publishDate | 2004-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-9ea18df498574f39b37c7d8e28977b722022-12-21T22:30:40ZengElsevierJournal of Lipid Research0022-22752004-03-01453456465Lipid transfer particle mediates the delivery of diacylglycerol from lipophorin to fat body in larval Manduca sextaLilian E. Canavoso0Hwa Kyung Yun1Zeina E. Jouni2Michael A. Wells3Department of Biochemistry and Molecular Biophysics and Center for Insect Science, Biological Sciences West, University of Arizona, Tucson, AZ 85721-0088Department of Biochemistry and Molecular Biophysics and Center for Insect Science, Biological Sciences West, University of Arizona, Tucson, AZ 85721-0088Department of Biochemistry and Molecular Biophysics and Center for Insect Science, Biological Sciences West, University of Arizona, Tucson, AZ 85721-0088Department of Biochemistry and Molecular Biophysics and Center for Insect Science, Biological Sciences West, University of Arizona, Tucson, AZ 85721-0088This work analyzed the process of lipid storage in fat body of larval Manduca sexta, focusing on the role of lipid transfer particle (LTP). Incubation of fat bodies with [3H]diacylglycerol-labeled lipophorin resulted in a significant accumulation of diacylglycerol (DAG) and triacylglycerol (TAG) in the tissue. Transfer of DAG to fat body and its storage as TAG was significantly inhibited (60%) by preincubating the tissue with anti-LTP antibody. Lipid transfer was restored to control values by adding LTP to fat body. Incubation of fat body with dual-labeled DAG lipophorin or its treatment with ammonium chloride showed that neither a membrane-bound lipoprotein lipase nor lipophorin endocytosis is a relevant pathway to transfer or to storage lipids into fat body, respectively. Treatment of fat body with suramin caused a 50% inhibition in [3H]DAG transfer from lipophorin. Treatment of [3H]DAG-labeled fat body with lipase significantly reduced the amount of [3H]DAG associated with the tissue, suggesting that the lipid is still on the external surface of the membrane.Whether this lipid represents irreversibly adsorbed lipophorin or a DAG lipase-sensitive pool is unknown. Nevertheless, these results indicate that the main pathway for DAG transfer from lipophorin to fat body is via LTP and receptor-mediated processes.http://www.sciencedirect.com/science/article/pii/S0022227520318733lipoprotein lipasedevelopmental stagestriacylglycerolendocytosis |
| spellingShingle | Lilian E. Canavoso Hwa Kyung Yun Zeina E. Jouni Michael A. Wells Lipid transfer particle mediates the delivery of diacylglycerol from lipophorin to fat body in larval Manduca sexta Journal of Lipid Research lipoprotein lipase developmental stages triacylglycerol endocytosis |
| title | Lipid transfer particle mediates the delivery of diacylglycerol from lipophorin to fat body in larval Manduca sexta |
| title_full | Lipid transfer particle mediates the delivery of diacylglycerol from lipophorin to fat body in larval Manduca sexta |
| title_fullStr | Lipid transfer particle mediates the delivery of diacylglycerol from lipophorin to fat body in larval Manduca sexta |
| title_full_unstemmed | Lipid transfer particle mediates the delivery of diacylglycerol from lipophorin to fat body in larval Manduca sexta |
| title_short | Lipid transfer particle mediates the delivery of diacylglycerol from lipophorin to fat body in larval Manduca sexta |
| title_sort | lipid transfer particle mediates the delivery of diacylglycerol from lipophorin to fat body in larval manduca sexta |
| topic | lipoprotein lipase developmental stages triacylglycerol endocytosis |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520318733 |
| work_keys_str_mv | AT lilianecanavoso lipidtransferparticlemediatesthedeliveryofdiacylglycerolfromlipophorintofatbodyinlarvalmanducasexta AT hwakyungyun lipidtransferparticlemediatesthedeliveryofdiacylglycerolfromlipophorintofatbodyinlarvalmanducasexta AT zeinaejouni lipidtransferparticlemediatesthedeliveryofdiacylglycerolfromlipophorintofatbodyinlarvalmanducasexta AT michaelawells lipidtransferparticlemediatesthedeliveryofdiacylglycerolfromlipophorintofatbodyinlarvalmanducasexta |