Investigation of urinary metabolomics in a phase I hookworm vaccine trial in Gabon
Metabolomics provides a powerful tool to study physiological changes in response to various perturbations such as vaccination. We explored whether metabolomic changes could be seen after vaccination in a phase I trial where Gabonese adults living either in rural or semi-urban areas received the subu...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9512193/?tool=EBI |
| _version_ | 1818030096431185920 |
|---|---|
| author | Madeleine Eunice Betouke Ongwe Yoanne D. Mouwenda Koen A. Stam Peter G. Kremsner Bertrand Lell David Diemert Jeff Bethony Maria E. Bottazzi Peter J. Hotez Remko V. Leeuwen Martin P. Grobusch Ayola A. Adegnika Oleg A. Mayboroda Maria Yazdanbakhsh |
| author_facet | Madeleine Eunice Betouke Ongwe Yoanne D. Mouwenda Koen A. Stam Peter G. Kremsner Bertrand Lell David Diemert Jeff Bethony Maria E. Bottazzi Peter J. Hotez Remko V. Leeuwen Martin P. Grobusch Ayola A. Adegnika Oleg A. Mayboroda Maria Yazdanbakhsh |
| author_sort | Madeleine Eunice Betouke Ongwe |
| collection | DOAJ |
| description | Metabolomics provides a powerful tool to study physiological changes in response to various perturbations such as vaccination. We explored whether metabolomic changes could be seen after vaccination in a phase I trial where Gabonese adults living either in rural or semi-urban areas received the subunit hookworm vaccine candidates (Na-GST-1 and Na-APR-1 (M74) adjuvanted with Alhydrogel plus GLA-AF (n = 24) or the hepatitis B vaccine (n = 8) as control. Urine samples were collected and assayed using targeted 1H NMR spectroscopy. At baseline, a set of metabolites significantly distinguished rural from semi-urban individuals. The pre- and post-vaccination comparisons indicated significant changes in few metabolites but only one day after the first vaccination. There was no relationship with immunogenicity. In conclusion, in a small phase 1 trial, urinary metabolomics could distinguish volunteers with different environmental exposures and reflected the safety of the vaccines but did not show a relationship to immunogenicity. |
| first_indexed | 2024-12-10T05:30:09Z |
| format | Article |
| id | doaj.art-9ea1b5f296ca46f29284b62d2fc62b27 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T05:30:09Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9ea1b5f296ca46f29284b62d2fc62b272022-12-22T02:00:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01179Investigation of urinary metabolomics in a phase I hookworm vaccine trial in GabonMadeleine Eunice Betouke OngweYoanne D. MouwendaKoen A. StamPeter G. KremsnerBertrand LellDavid DiemertJeff BethonyMaria E. BottazziPeter J. HotezRemko V. LeeuwenMartin P. GrobuschAyola A. AdegnikaOleg A. MayborodaMaria YazdanbakhshMetabolomics provides a powerful tool to study physiological changes in response to various perturbations such as vaccination. We explored whether metabolomic changes could be seen after vaccination in a phase I trial where Gabonese adults living either in rural or semi-urban areas received the subunit hookworm vaccine candidates (Na-GST-1 and Na-APR-1 (M74) adjuvanted with Alhydrogel plus GLA-AF (n = 24) or the hepatitis B vaccine (n = 8) as control. Urine samples were collected and assayed using targeted 1H NMR spectroscopy. At baseline, a set of metabolites significantly distinguished rural from semi-urban individuals. The pre- and post-vaccination comparisons indicated significant changes in few metabolites but only one day after the first vaccination. There was no relationship with immunogenicity. In conclusion, in a small phase 1 trial, urinary metabolomics could distinguish volunteers with different environmental exposures and reflected the safety of the vaccines but did not show a relationship to immunogenicity.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9512193/?tool=EBI |
| spellingShingle | Madeleine Eunice Betouke Ongwe Yoanne D. Mouwenda Koen A. Stam Peter G. Kremsner Bertrand Lell David Diemert Jeff Bethony Maria E. Bottazzi Peter J. Hotez Remko V. Leeuwen Martin P. Grobusch Ayola A. Adegnika Oleg A. Mayboroda Maria Yazdanbakhsh Investigation of urinary metabolomics in a phase I hookworm vaccine trial in Gabon PLoS ONE |
| title | Investigation of urinary metabolomics in a phase I hookworm vaccine trial in Gabon |
| title_full | Investigation of urinary metabolomics in a phase I hookworm vaccine trial in Gabon |
| title_fullStr | Investigation of urinary metabolomics in a phase I hookworm vaccine trial in Gabon |
| title_full_unstemmed | Investigation of urinary metabolomics in a phase I hookworm vaccine trial in Gabon |
| title_short | Investigation of urinary metabolomics in a phase I hookworm vaccine trial in Gabon |
| title_sort | investigation of urinary metabolomics in a phase i hookworm vaccine trial in gabon |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9512193/?tool=EBI |
| work_keys_str_mv | AT madeleineeunicebetoukeongwe investigationofurinarymetabolomicsinaphaseihookwormvaccinetrialingabon AT yoannedmouwenda investigationofurinarymetabolomicsinaphaseihookwormvaccinetrialingabon AT koenastam investigationofurinarymetabolomicsinaphaseihookwormvaccinetrialingabon AT petergkremsner investigationofurinarymetabolomicsinaphaseihookwormvaccinetrialingabon AT bertrandlell investigationofurinarymetabolomicsinaphaseihookwormvaccinetrialingabon AT daviddiemert investigationofurinarymetabolomicsinaphaseihookwormvaccinetrialingabon AT jeffbethony investigationofurinarymetabolomicsinaphaseihookwormvaccinetrialingabon AT mariaebottazzi investigationofurinarymetabolomicsinaphaseihookwormvaccinetrialingabon AT peterjhotez investigationofurinarymetabolomicsinaphaseihookwormvaccinetrialingabon AT remkovleeuwen investigationofurinarymetabolomicsinaphaseihookwormvaccinetrialingabon AT martinpgrobusch investigationofurinarymetabolomicsinaphaseihookwormvaccinetrialingabon AT ayolaaadegnika investigationofurinarymetabolomicsinaphaseihookwormvaccinetrialingabon AT olegamayboroda investigationofurinarymetabolomicsinaphaseihookwormvaccinetrialingabon AT mariayazdanbakhsh investigationofurinarymetabolomicsinaphaseihookwormvaccinetrialingabon |