A novel role for DNA methyltransferase 1 in regulating oocyte cytoplasmic maturation in pigs.
Maternal factors are required for oocyte maturation and embryo development. To better understand the role of DNA methyltransferase 1 (Dnmt1) in oocyte maturation and embryo development, small interfering RNA (siRNA) was conducted in porcine oocytes. In this study, our results showed that Dnmt1 local...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0127512 |
| _version_ | 1827379495597768704 |
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| author | Yanjun Huan Bingteng Xie Shichao Liu Qingran Kong Zhonghua Liu |
| author_facet | Yanjun Huan Bingteng Xie Shichao Liu Qingran Kong Zhonghua Liu |
| author_sort | Yanjun Huan |
| collection | DOAJ |
| description | Maternal factors are required for oocyte maturation and embryo development. To better understand the role of DNA methyltransferase 1 (Dnmt1) in oocyte maturation and embryo development, small interfering RNA (siRNA) was conducted in porcine oocytes. In this study, our results showed that Dnmt1 localized in oocyte cytoplasm and its expression displayed no obvious change during oocyte maturation. When siRNAs targeting Dnmt1 were injected into germinal vesicle (GV) stage oocytes, Dnmt1 transcripts significantly decreased in matured oocytes (P<0.05). After Dnmt1 knockdown in GV stage oocytes, the significant reduction of glutathione content, mitochondrial DNA copy number, glucose-6-phosphate dehydrogenase activity and expression profiles of maternal factors and the severely disrupted distribution of cortical granules were observed in MII stage oocytes (P<0.05), leading to the impaired oocyte cytoplasm. Further study displayed that Dnmt1 knockdown in GV stage oocytes significantly reduced the development of early embryos generated through parthenogenetic activation, in vitro fertilization and somatic cell nuclear transfer (P<0.05). In conclusion, Dnmt1 was indispensable for oocyte cytoplasmic maturation, providing a novel role for Dnmt1 in the regulation of oocyte maturation. |
| first_indexed | 2024-03-08T13:16:32Z |
| format | Article |
| id | doaj.art-9eaaccb3aeb147988c1aa213b4836c36 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-08T13:16:32Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj.art-9eaaccb3aeb147988c1aa213b4836c362024-01-18T06:25:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01105e012751210.1371/journal.pone.0127512A novel role for DNA methyltransferase 1 in regulating oocyte cytoplasmic maturation in pigs.Yanjun HuanBingteng XieShichao LiuQingran KongZhonghua LiuMaternal factors are required for oocyte maturation and embryo development. To better understand the role of DNA methyltransferase 1 (Dnmt1) in oocyte maturation and embryo development, small interfering RNA (siRNA) was conducted in porcine oocytes. In this study, our results showed that Dnmt1 localized in oocyte cytoplasm and its expression displayed no obvious change during oocyte maturation. When siRNAs targeting Dnmt1 were injected into germinal vesicle (GV) stage oocytes, Dnmt1 transcripts significantly decreased in matured oocytes (P<0.05). After Dnmt1 knockdown in GV stage oocytes, the significant reduction of glutathione content, mitochondrial DNA copy number, glucose-6-phosphate dehydrogenase activity and expression profiles of maternal factors and the severely disrupted distribution of cortical granules were observed in MII stage oocytes (P<0.05), leading to the impaired oocyte cytoplasm. Further study displayed that Dnmt1 knockdown in GV stage oocytes significantly reduced the development of early embryos generated through parthenogenetic activation, in vitro fertilization and somatic cell nuclear transfer (P<0.05). In conclusion, Dnmt1 was indispensable for oocyte cytoplasmic maturation, providing a novel role for Dnmt1 in the regulation of oocyte maturation.https://doi.org/10.1371/journal.pone.0127512 |
| spellingShingle | Yanjun Huan Bingteng Xie Shichao Liu Qingran Kong Zhonghua Liu A novel role for DNA methyltransferase 1 in regulating oocyte cytoplasmic maturation in pigs. PLoS ONE |
| title | A novel role for DNA methyltransferase 1 in regulating oocyte cytoplasmic maturation in pigs. |
| title_full | A novel role for DNA methyltransferase 1 in regulating oocyte cytoplasmic maturation in pigs. |
| title_fullStr | A novel role for DNA methyltransferase 1 in regulating oocyte cytoplasmic maturation in pigs. |
| title_full_unstemmed | A novel role for DNA methyltransferase 1 in regulating oocyte cytoplasmic maturation in pigs. |
| title_short | A novel role for DNA methyltransferase 1 in regulating oocyte cytoplasmic maturation in pigs. |
| title_sort | novel role for dna methyltransferase 1 in regulating oocyte cytoplasmic maturation in pigs |
| url | https://doi.org/10.1371/journal.pone.0127512 |
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