Cytotoxicity evaluation of Launaea procumbens on two cell lines
Background and objectives: Launaea procumbens is a member of Asteraceae family which can be found in the south part of Iran especially the province of Hormozgan. This plant has got a variety of usage in traditional medicine for curing the skin diseases. In this study, we have investigated the cytoto...
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Iranian Society of Pharmacognosy
2017-11-01
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| Series: | Research Journal of Pharmacognosy |
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| Online Access: | http://www.rjpharmacognosy.ir/article_52983_b3e6ebfe92bb758ea013e6ea4c4fcc35.pdf |
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| author | M. Khomayezian N. Rahimifard F. Mojab F. Bagheri S.R. Pakzad* |
| author_facet | M. Khomayezian N. Rahimifard F. Mojab F. Bagheri S.R. Pakzad* |
| author_sort | M. Khomayezian |
| collection | DOAJ |
| description | Background and objectives: Launaea procumbens is a member of Asteraceae family which can be found in the south part of Iran especially the province of Hormozgan. This plant has got a variety of usage in traditional medicine for curing the skin diseases. In this study, we have investigated the cytotoxic effect of its various extracts at concentrations bracketing the minimum antibacterial concentration that has been found in a previous study to be 450 ug/mL. Methods: Total methanol extract was prepared with the standard method of maceration. Different fractions were prepared by liquid-liquid fractionation. The extracts were then dried with rotary evaporator. The dried concentrated extracts were kept within glass vials under standard conditions until used. Hep-2c and VERO cell lines were used in MTT test. A range of concentrations (10-500 ug/mL) of the extracts were prepared and were added to about 70% confluent 96 well plates. After exposure for 48 h, MTT solution was added to the wells, and 4 h later formazan crystals were solubilized and optical densities were read at 570 nm. Results: The MTT results indicated that total methanol extract and methanol and petroleum ether fractions of this plant showed acceptable safety at concentrations that were already shown to be bactericidal. Conclusion: According to the results L. procumbens extract and fractions can be used at concentrations up to 500 ug/mL. |
| first_indexed | 2024-04-14T02:44:53Z |
| format | Article |
| id | doaj.art-9eabf7e879a44483aae88334b47f751d |
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| issn | 2345-4458 2345-5977 |
| language | English |
| last_indexed | 2024-04-14T02:44:53Z |
| publishDate | 2017-11-01 |
| publisher | Iranian Society of Pharmacognosy |
| record_format | Article |
| series | Research Journal of Pharmacognosy |
| spelling | doaj.art-9eabf7e879a44483aae88334b47f751d2022-12-22T02:16:35ZengIranian Society of PharmacognosyResearch Journal of Pharmacognosy2345-44582345-59772017-11-014Supplement6652983Cytotoxicity evaluation of Launaea procumbens on two cell linesM. Khomayezian0N. Rahimifard1F. Mojab2F. Bagheri3S.R. Pakzad*4Pharmacy Sciences Branch, Islamic Azad University, Tehran, Iran.Microbiology Lab, Food and Drug Control Laboratory, Ministry of Health, Tehran, Iran.Pharmacy Faculty, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Pharmacy Sciences Branch, Islamic Azad University, Tehran, Iran.Biologicals Lab, Food and Drug Control Laboratory, Ministry of Health, Tehran, Iran.Background and objectives: Launaea procumbens is a member of Asteraceae family which can be found in the south part of Iran especially the province of Hormozgan. This plant has got a variety of usage in traditional medicine for curing the skin diseases. In this study, we have investigated the cytotoxic effect of its various extracts at concentrations bracketing the minimum antibacterial concentration that has been found in a previous study to be 450 ug/mL. Methods: Total methanol extract was prepared with the standard method of maceration. Different fractions were prepared by liquid-liquid fractionation. The extracts were then dried with rotary evaporator. The dried concentrated extracts were kept within glass vials under standard conditions until used. Hep-2c and VERO cell lines were used in MTT test. A range of concentrations (10-500 ug/mL) of the extracts were prepared and were added to about 70% confluent 96 well plates. After exposure for 48 h, MTT solution was added to the wells, and 4 h later formazan crystals were solubilized and optical densities were read at 570 nm. Results: The MTT results indicated that total methanol extract and methanol and petroleum ether fractions of this plant showed acceptable safety at concentrations that were already shown to be bactericidal. Conclusion: According to the results L. procumbens extract and fractions can be used at concentrations up to 500 ug/mL.http://www.rjpharmacognosy.ir/article_52983_b3e6ebfe92bb758ea013e6ea4c4fcc35.pdfcytotoxicityLaunaea procumbensMTT |
| spellingShingle | M. Khomayezian N. Rahimifard F. Mojab F. Bagheri S.R. Pakzad* Cytotoxicity evaluation of Launaea procumbens on two cell lines Research Journal of Pharmacognosy cytotoxicity Launaea procumbens MTT |
| title | Cytotoxicity evaluation of Launaea procumbens on two cell lines |
| title_full | Cytotoxicity evaluation of Launaea procumbens on two cell lines |
| title_fullStr | Cytotoxicity evaluation of Launaea procumbens on two cell lines |
| title_full_unstemmed | Cytotoxicity evaluation of Launaea procumbens on two cell lines |
| title_short | Cytotoxicity evaluation of Launaea procumbens on two cell lines |
| title_sort | cytotoxicity evaluation of launaea procumbens on two cell lines |
| topic | cytotoxicity Launaea procumbens MTT |
| url | http://www.rjpharmacognosy.ir/article_52983_b3e6ebfe92bb758ea013e6ea4c4fcc35.pdf |
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