Invitro inhibitory effect of Lactic acid bacteria isolated from Kunun zaki against multi-drug resistant diarrhogenic bacteria in HIV patients in Jos, Nigeria
Background: Claims from locals in Nigeria hold that Kunun zaki has some medicinal properties. The study was therefore carried out to investigate the inhibitory effect of lactic acid bacteria (LAB) on multidrug resistant diarrheagenic bacteria in human immunodeficiency virus (HIV) patients. Method: T...
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| Format: | Article |
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Zagazig University, Faculty of Medicine
2023-08-01
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| Series: | Microbes and Infectious Diseases |
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| Online Access: | https://mid.journals.ekb.eg/article_287014_73c1a579082fb62bc18bff034c39eaf2.pdf |
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| author | Anayochukwu Ngene Solomon Ebuga John Egbere Collins Odu Margaret Danladi Isaac Onyimba Michael Dashen Edo Ochiheche Hemen Nguhemen Amos Ogaji |
| author_facet | Anayochukwu Ngene Solomon Ebuga John Egbere Collins Odu Margaret Danladi Isaac Onyimba Michael Dashen Edo Ochiheche Hemen Nguhemen Amos Ogaji |
| author_sort | Anayochukwu Ngene |
| collection | DOAJ |
| description | Background: Claims from locals in Nigeria hold that Kunun zaki has some medicinal properties. The study was therefore carried out to investigate the inhibitory effect of lactic acid bacteria (LAB) on multidrug resistant diarrheagenic bacteria in human immunodeficiency virus (HIV) patients. Method: Twenty-five stool samples of seropositive HIV patients from Plateau State Specialist Hospital confirmed to have chronic diarrhea were collected aseptically and bacteria were isolated and identified using microscopic and biochemical techniques. The antibiotics susceptibility tests of the isolates were also carried out using the disc diffusion method to determine drug resistance of the bacteria. The lactic acid bacteria (LAB) used were isolated and identified using standard bacteriological techniques and analytical profile index (API) kits. Diarrheagenic bacteria which showed multiple resistance to antibiotics were tested against lactic acid bacteria using agar well diffusion method. Results: The results showed that Shigella spp (36.0%), Salmonella spp (16.0%) and Escherichia coli (E. coli) (48.0%) were the diarrheagenic bacteria isolated from the HIV patients. The pathogens were most resistant to ampicillin (60%) and least resistant to tarivid (8%). Lactobacillus brevis, Lactobacillus lactis, Lactobacillus casei and Lactobacillus plantarum isolated from Kunun zaki demonstrated antibacterial activity against the pathogens with the effect of the two lactic acid bacteria (L. lactis Gb3ii and L. plantarum Ar1) being significantly higher than the individual LAB used respectively. Conclusion: Lactic acid bacteria from Kunun zaki had demonstrated antibacterial effects against multidrug resistant pathogens, hence could be potential probiotics for inclusion in the fermentation of Kunun zaki that HIV patients could consume. |
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| id | doaj.art-9eaccafd86644ccf9e5cea6b3d68708a |
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| issn | 2682-4132 2682-4140 |
| language | English |
| last_indexed | 2024-03-12T15:15:11Z |
| publishDate | 2023-08-01 |
| publisher | Zagazig University, Faculty of Medicine |
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| series | Microbes and Infectious Diseases |
| spelling | doaj.art-9eaccafd86644ccf9e5cea6b3d68708a2023-08-11T15:20:52ZengZagazig University, Faculty of MedicineMicrobes and Infectious Diseases2682-41322682-41402023-08-01431008102110.21608/mid.2023.175533.1418287014Invitro inhibitory effect of Lactic acid bacteria isolated from Kunun zaki against multi-drug resistant diarrhogenic bacteria in HIV patients in Jos, NigeriaAnayochukwu Ngene0Solomon Ebuga1John Egbere2Collins Odu3Margaret Danladi4Isaac Onyimba5Michael Dashen6Edo Ochiheche7Hemen Nguhemen8Amos Ogaji9Department of Microbiology, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, Nigeria.Department of Microbiology, Faculty of Natural Sciences, University of Jos, Jos, NigeriaDepartment of Microbiology, Faculty of Natural Sciences, University of Jos, Jos, NigeriaDepartment of Biological Sciences, Faculty of Natural Sciences, University of Maiduguri, Nigeria.Department of Microbiology, Plateau State University, Bokkos, Nigeria.Department of Science Laboratory Technology, Faculty of Natural Sciences, University of Jos, Jos, Nigeria.Department of Microbiology, Faculty of Natural Sciences, University of Jos, Jos, NigeriaDepartment of Science Laboratory Technology, Fidei Polytechnic, Gboko, Nigeria.Department of Science Laboratory Technology, Fidei Polytechnic, Gboko, Nigeria.Department of Microbiology, Faculty of Natural Sciences, University of Jos, Jos, NigeriaBackground: Claims from locals in Nigeria hold that Kunun zaki has some medicinal properties. The study was therefore carried out to investigate the inhibitory effect of lactic acid bacteria (LAB) on multidrug resistant diarrheagenic bacteria in human immunodeficiency virus (HIV) patients. Method: Twenty-five stool samples of seropositive HIV patients from Plateau State Specialist Hospital confirmed to have chronic diarrhea were collected aseptically and bacteria were isolated and identified using microscopic and biochemical techniques. The antibiotics susceptibility tests of the isolates were also carried out using the disc diffusion method to determine drug resistance of the bacteria. The lactic acid bacteria (LAB) used were isolated and identified using standard bacteriological techniques and analytical profile index (API) kits. Diarrheagenic bacteria which showed multiple resistance to antibiotics were tested against lactic acid bacteria using agar well diffusion method. Results: The results showed that Shigella spp (36.0%), Salmonella spp (16.0%) and Escherichia coli (E. coli) (48.0%) were the diarrheagenic bacteria isolated from the HIV patients. The pathogens were most resistant to ampicillin (60%) and least resistant to tarivid (8%). Lactobacillus brevis, Lactobacillus lactis, Lactobacillus casei and Lactobacillus plantarum isolated from Kunun zaki demonstrated antibacterial activity against the pathogens with the effect of the two lactic acid bacteria (L. lactis Gb3ii and L. plantarum Ar1) being significantly higher than the individual LAB used respectively. Conclusion: Lactic acid bacteria from Kunun zaki had demonstrated antibacterial effects against multidrug resistant pathogens, hence could be potential probiotics for inclusion in the fermentation of Kunun zaki that HIV patients could consume.https://mid.journals.ekb.eg/article_287014_73c1a579082fb62bc18bff034c39eaf2.pdfkunun zakimultidrug resistant bacterialactic acid bacteriahiv patients |
| spellingShingle | Anayochukwu Ngene Solomon Ebuga John Egbere Collins Odu Margaret Danladi Isaac Onyimba Michael Dashen Edo Ochiheche Hemen Nguhemen Amos Ogaji Invitro inhibitory effect of Lactic acid bacteria isolated from Kunun zaki against multi-drug resistant diarrhogenic bacteria in HIV patients in Jos, Nigeria Microbes and Infectious Diseases kunun zaki multidrug resistant bacteria lactic acid bacteria hiv patients |
| title | Invitro inhibitory effect of Lactic acid bacteria isolated from Kunun zaki against multi-drug resistant diarrhogenic bacteria in HIV patients in Jos, Nigeria |
| title_full | Invitro inhibitory effect of Lactic acid bacteria isolated from Kunun zaki against multi-drug resistant diarrhogenic bacteria in HIV patients in Jos, Nigeria |
| title_fullStr | Invitro inhibitory effect of Lactic acid bacteria isolated from Kunun zaki against multi-drug resistant diarrhogenic bacteria in HIV patients in Jos, Nigeria |
| title_full_unstemmed | Invitro inhibitory effect of Lactic acid bacteria isolated from Kunun zaki against multi-drug resistant diarrhogenic bacteria in HIV patients in Jos, Nigeria |
| title_short | Invitro inhibitory effect of Lactic acid bacteria isolated from Kunun zaki against multi-drug resistant diarrhogenic bacteria in HIV patients in Jos, Nigeria |
| title_sort | invitro inhibitory effect of lactic acid bacteria isolated from kunun zaki against multi drug resistant diarrhogenic bacteria in hiv patients in jos nigeria |
| topic | kunun zaki multidrug resistant bacteria lactic acid bacteria hiv patients |
| url | https://mid.journals.ekb.eg/article_287014_73c1a579082fb62bc18bff034c39eaf2.pdf |
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