Exposure to salinity induces oxidative damage and changes in the expression of genes related to appetite regulation in Nile tilapia (Oreochromis niloticus)
Variations in water salinity and other extrinsic factors have been shown to induce changes in feeding rhythms and growth in fish. However, it is unknown whether appetite-related hormones mediate these changes in Nile tilapia (Oreochromis niloticus), an important species for aquaculture in several co...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.948228/full |
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| author | Amanda W. S. Martins Eduardo N. Dellagostin Eduardo B. Blödorn Tony Leandro R. Silveira Luis A. Sampaio Eliza R. Komninou Antonio S. Varela Junior Carine D. Corcini Leandro S. Nunes Mariana H. Remião Gilberto L. Collares William B. Domingues Vinicius F. Campos |
| author_facet | Amanda W. S. Martins Eduardo N. Dellagostin Eduardo B. Blödorn Tony Leandro R. Silveira Luis A. Sampaio Eliza R. Komninou Antonio S. Varela Junior Carine D. Corcini Leandro S. Nunes Mariana H. Remião Gilberto L. Collares William B. Domingues Vinicius F. Campos |
| author_sort | Amanda W. S. Martins |
| collection | DOAJ |
| description | Variations in water salinity and other extrinsic factors have been shown to induce changes in feeding rhythms and growth in fish. However, it is unknown whether appetite-related hormones mediate these changes in Nile tilapia (Oreochromis niloticus), an important species for aquaculture in several countries. This study aimed to evaluate the expression of genes responsible for appetite regulation and genes related to metabolic and physiological changes in tilapia exposed to different salinities. Moreover, the study proposed to sequence and to characterize the cart, cck, and pyy genes, and to quantify their expression in the brain and intestine of the fish by quantitative polymerase chain reaction (qPCR). The animals were exposed to three salinities: 0, 6, and 12 parts per thousand (ppt) of salt for 21 days. Furthermore, lipid peroxidation, reactive oxygen species, DNA damage, and membrane fluidity in blood cells were quantified by flow cytometry. The results indicated an increased expression of cart, pyy, and cck and a decreased expression of npy in the brain, and the same with cck and npy in the intestine of fish treated with 12 ppt. This modulation and other adaptive responses may have contributed to the decrease in weight gain, specific growth rate, and final weight. In addition, we showed oxidative damage in blood cells resulting from increasing salinity. These results provide essential data on O. niloticus when exposed to high salinities that have never been described before and generate knowledge necessary for developing biotechnologies that may help improve the production of economically important farmed fish. |
| first_indexed | 2024-12-10T11:28:01Z |
| format | Article |
| id | doaj.art-9eb3d229835a4871beb22075f8474d4a |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-10T11:28:01Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-9eb3d229835a4871beb22075f8474d4a2022-12-22T01:50:40ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-09-011310.3389/fgene.2022.948228948228Exposure to salinity induces oxidative damage and changes in the expression of genes related to appetite regulation in Nile tilapia (Oreochromis niloticus)Amanda W. S. Martins0Eduardo N. Dellagostin1Eduardo B. Blödorn2Tony Leandro R. Silveira3Luis A. Sampaio4Eliza R. Komninou5Antonio S. Varela Junior6Carine D. Corcini7Leandro S. Nunes8Mariana H. Remião9Gilberto L. Collares10William B. Domingues11Vinicius F. Campos12Laboratório de Genômica Estrutural, Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, BrazilLaboratório de Genômica Estrutural, Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, BrazilLaboratório de Genômica Estrutural, Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, BrazilInstituto de Ciências Biológicas, Universidade Federal do Rio Grande, Rio Grande, RS, BrazilLaboratório de Piscicultura Estuarina e Marinha, Programa de Pós-graduação em Aquicultura, Instituto de Oceanografia, Universidade Federal do Rio Grande, Rio Grande, RS, BrazilLaboratório de Genômica Estrutural, Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, BrazilLaboratório de Reprodução Animal, Programa de Pós-Graduação em Biologia de Ambientes Aquáticos Continentais, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Rio Grande, RS, BrazilReproPel, Programa de Pós-Graduação em Veterinária, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, BrazilLaboratório de Genômica Estrutural, Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, BrazilLaboratório de Genômica Estrutural, Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, BrazilAgência de Desenvolvimento da Bacia da Lagoa Mirim, Universidade Federal de Pelotas, Pelotas, RS, BrazilLaboratório de Genômica Estrutural, Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, BrazilLaboratório de Genômica Estrutural, Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, BrazilVariations in water salinity and other extrinsic factors have been shown to induce changes in feeding rhythms and growth in fish. However, it is unknown whether appetite-related hormones mediate these changes in Nile tilapia (Oreochromis niloticus), an important species for aquaculture in several countries. This study aimed to evaluate the expression of genes responsible for appetite regulation and genes related to metabolic and physiological changes in tilapia exposed to different salinities. Moreover, the study proposed to sequence and to characterize the cart, cck, and pyy genes, and to quantify their expression in the brain and intestine of the fish by quantitative polymerase chain reaction (qPCR). The animals were exposed to three salinities: 0, 6, and 12 parts per thousand (ppt) of salt for 21 days. Furthermore, lipid peroxidation, reactive oxygen species, DNA damage, and membrane fluidity in blood cells were quantified by flow cytometry. The results indicated an increased expression of cart, pyy, and cck and a decreased expression of npy in the brain, and the same with cck and npy in the intestine of fish treated with 12 ppt. This modulation and other adaptive responses may have contributed to the decrease in weight gain, specific growth rate, and final weight. In addition, we showed oxidative damage in blood cells resulting from increasing salinity. These results provide essential data on O. niloticus when exposed to high salinities that have never been described before and generate knowledge necessary for developing biotechnologies that may help improve the production of economically important farmed fish.https://www.frontiersin.org/articles/10.3389/fgene.2022.948228/fullCARTPYYNPYCCKgene expressionfood intake |
| spellingShingle | Amanda W. S. Martins Eduardo N. Dellagostin Eduardo B. Blödorn Tony Leandro R. Silveira Luis A. Sampaio Eliza R. Komninou Antonio S. Varela Junior Carine D. Corcini Leandro S. Nunes Mariana H. Remião Gilberto L. Collares William B. Domingues Vinicius F. Campos Exposure to salinity induces oxidative damage and changes in the expression of genes related to appetite regulation in Nile tilapia (Oreochromis niloticus) Frontiers in Genetics CART PYY NPY CCK gene expression food intake |
| title | Exposure to salinity induces oxidative damage and changes in the expression of genes related to appetite regulation in Nile tilapia (Oreochromis niloticus) |
| title_full | Exposure to salinity induces oxidative damage and changes in the expression of genes related to appetite regulation in Nile tilapia (Oreochromis niloticus) |
| title_fullStr | Exposure to salinity induces oxidative damage and changes in the expression of genes related to appetite regulation in Nile tilapia (Oreochromis niloticus) |
| title_full_unstemmed | Exposure to salinity induces oxidative damage and changes in the expression of genes related to appetite regulation in Nile tilapia (Oreochromis niloticus) |
| title_short | Exposure to salinity induces oxidative damage and changes in the expression of genes related to appetite regulation in Nile tilapia (Oreochromis niloticus) |
| title_sort | exposure to salinity induces oxidative damage and changes in the expression of genes related to appetite regulation in nile tilapia oreochromis niloticus |
| topic | CART PYY NPY CCK gene expression food intake |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.948228/full |
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